LOCUS       sequence-356089-       15176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 module containing mCHERRY-nYFP HSP21-cYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, 
            Crete P, Field B
  TITLE     MoBiFC: development of a modular bimolecular fluorescence 
            complementation toolkit for the analysis of chloroplast 
            protein-protein interactions.
  JOURNAL   Plant Methods. 2022 May 26;18(1):69. doi: 
            10.1186/s13007-022-00902-1.
  PUBMED    35619173
REFERENCE   2  (bases 1 to 15176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2022-05-26"; volume: "18(1):69. doi"; pages: " 
            10.1186/s13007-022-00902-1"
COMMENT     Sequence Label: pLBM0050
FEATURES             Location/Qualifiers
     source          1..15176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1010..1355
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1977..2229
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3245..3590
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4030..4737
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4175..4193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             4801..4824
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    5381..5555
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        6568..6913
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8106..8195
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     polyA_signal    8236..8410
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        9423..9768
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             9873..9893
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9903..9905,9906..9908,9909..10622)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9948..9969)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      10635..10887
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10953..10977
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      11065..11775
                     /label=oriV
                     /note="incP origin of replication"
     promoter        11796..11900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11901..12695
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12866..13454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13355..13374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13790..14938
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    15084..15108
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgccgaattc ggatccggag gaattccaat cccacaaaaa tctgagctta acagcacagt
       61 tgctcctctc agagcagaat cgggtattca acaccctcat atcaactact acgttgtgta
      121 taacggtcca catgccggta tatacgatga ctggggttgt acaaaggcgg caacaaacgg
      181 cgttcccgga gttgcacaca agaaatttgc cactattaca gaggcaagag cagcagctga
      241 cgcgtacaca acaagtcagc aaacagacag gttgaacttc atccccaaag gagaagctca
      301 actcaagccc aagagctttg ctaaggccct aacaagccca ccaaagcaaa aagcccactg
      361 gctcacgcta ggaaccaaaa ggcccagcag tgatccagcc ccaaaagaga tctcctttgc
      421 cccggagatt acaatggacg atttcctcta tctttacgat ctaggaagga agttcgaagg
      481 tgaaggtgac gacactatgt tcaccactga taatgagaag gttagcctct tcaatttcag
      541 aaagaatgct gacccacaga tggttagaga ggcctacgca gcaagtctca tcaagacgat
      601 ctacccgagt aacaatctcc aggagatcaa ataccttccc aagaaggtta aagatgcagt
      661 caaaagattc aggactaatt gcatcaagaa cacagagaaa gacatatttc tcaagatcag
      721 aagtactatt ccagtatgga cgattcaagg cttgcttcat aaaccaaggc aagtaataga
      781 gattggagtc tctaaaaagg tagttcctac tgaatctaag gccatgcatg gagtctaaga
      841 ttcaaatcga ggatctaaca gaactcgccg tcaagactgg cgaacagttc atacagagtc
      901 ttttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctgg
      961 tctactccaa aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc
     1021 aacaaaggat aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     1081 tcgaaaggac agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
     1141 aggctatcat tcaagatctc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     1201 ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac aaagcaagtg gattgatgtg
     1261 acatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
     1321 ctatataagg aagttcattt catttggaga ggacacgctc gagtataaga gctcattttt
     1381 acaacaatta ccaacaacaa caaacaacaa acaacattac aattacattt acaattatcg
     1441 atacaatgga acgagctata caaggaaacg atgctaggga acaagcttat ggtgaacgtt
     1501 ggaatggagg atcaggaagt tccacttctc ccttcaaact tcctgacgaa agtccgagtt
     1561 ggactgagtg gcggctacat aacgatgaga ctatttcgaa tcaagataat ccccttggtt
     1621 tcaaggaaag ctggggtttc gggaaagttg tatttaagag atatctcaga tacgacggga
     1681 cggaaacttc actgcacaga gtccttggat cttggacggg agattcggtt aactatgcag
     1741 catctcgatt tctcggtttc gaccagatcg gatgtaccta tagtattcgg tttcgaggag
     1801 ttagtgtcac catttctgga gggtcgcgaa ctcttcagca tctcagtgaa atggcaattc
     1861 ggtctaagca agaactgcta cagcttaccc cagtcaaagt ggaaagtgat gtatcaagag
     1921 gatgccctga aggtgttgaa accttcgaag aagaaagcga gtaagcttgt caagcagatc
     1981 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     2041 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     2101 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     2161 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     2221 tactagatcg acgctgcaag aattcaagct tggaggaatt ccaatcccac aaaaatctga
     2281 gcttaacagc acagttgctc ctctcagagc agaatcgggt attcaacacc ctcatatcaa
     2341 ctactacgtt gtgtataacg gtccacatgc cggtatatac gatgactggg gttgtacaaa
     2401 ggcggcaaca aacggcgttc ccggagttgc acacaagaaa tttgccacta ttacagaggc
     2461 aagagcagca gctgacgcgt acacaacaag tcagcaaaca gacaggttga acttcatccc
     2521 caaaggagaa gctcaactca agcccaagag ctttgctaag gccctaacaa gcccaccaaa
     2581 gcaaaaagcc cactggctca cgctaggaac caaaaggccc agcagtgatc cagccccaaa
     2641 agagatctcc tttgccccgg agattacaat ggacgatttc ctctatcttt acgatctagg
     2701 aaggaagttc gaaggtgaag gtgacgacac tatgttcacc actgataatg agaaggttag
     2761 cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct acgcagcaag
     2821 tctcatcaag acgatctacc cgagtaacaa tctccaggag atcaaatacc ttcccaagaa
     2881 ggttaaagat gcagtcaaaa gattcaggac taattgcatc aagaacacag agaaagacat
     2941 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcataaacc
     3001 aaggcaagta atagagattg gagtctctaa aaaggtagtt cctactgaat ctaaggccat
     3061 gcatggagtc taagattcaa atcgaggatc taacagaact cgccgtcaag actggcgaac
     3121 agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg
     3181 agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg
     3241 ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag
     3301 ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
     3361 attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg
     3421 gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc
     3481 aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc cactatcctt
     3541 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctcgagta
     3601 taagagctca tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta
     3661 catttacaat tatcgataca atggcttcct ctatgctctc ttccgctact atggttgcct
     3721 ctccggctca ggccactatg gtcgctcctt tcaacggact taagtcctcc gctgccttcc
     3781 cagccacccg caaggctaac aacgacatta cttccatcac aagcaacggc ggaagagtta
     3841 actgcatgca ggtcatttat atttcttctt tcactttttt attattccat atgatttttt
     3901 tcggttcttt cttcgaatct acataaacta atatcattgg aaaaatcgaa aaaataggtg
     3961 tggcctccga ttggaaagaa gaagtttgag actctctctt accttcctga ccttaccgat
     4021 tccgaaggta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     4081 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     4141 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     4201 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     4261 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     4321 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     4381 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     4441 ggccccgtaa tgcagaagaa aaccatgggc tgggaggcct cctccgagcg gatgtacccc
     4501 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     4561 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     4621 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     4681 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagggt
     4741 tcggattaca aggaccacga cggggactac aaggatcatg atttggatgc tgctgccgcc
     4801 gactataagg atgatgacga taaggtgagc aagggcgaag agttgttcac tggtgtggtt
     4861 cctatcctcg ttgagcttga tggtgatgtg aacgggcaca agttctccgt ttctggtgaa
     4921 ggtgagggtg atgctactta cggaaagctc accctcaagt tcatctgtac cactggaaag
     4981 ctccctgtgc cttggcctac tctcgttact actttcggat acggactcca gtgcttcgct
     5041 agataccctg atcatatgaa gcagcacgac ttcttcaaga gcgctatgcc tgagggatac
     5101 gtgcaagaga gaaccatttt cttcaaggac gacggcaact acaagaccag agctgaggtt
     5161 aagttcgaag gtgacaccct cgtgaacagg atcgagctta agggcatcga cttcaaagag
     5221 gacggaaaca tcctcggaca caagctcgag tacaactaca acagccacaa cgtgtacatc
     5281 atggccgaca agcagaagaa cggcatcaag gtcaacttca agatcaggca caacatcgag
     5341 gactaagctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     5401 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     5461 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     5521 tcctaaaacc aaaatccagt actaaaatcc agatcgctac tagaattcga gctcggagga
     5581 attccaatcc cacaaaaatc tgagcttaac agcacagttg ctcctctcag agcagaatcg
     5641 ggtattcaac accctcatat caactactac gttgtgtata acggtccaca tgccggtata
     5701 tacgatgact ggggttgtac aaaggcggca acaaacggcg ttcccggagt tgcacacaag
     5761 aaatttgcca ctattacaga ggcaagagca gcagctgacg cgtacacaac aagtcagcaa
     5821 acagacaggt tgaacttcat ccccaaagga gaagctcaac tcaagcccaa gagctttgct
     5881 aaggccctaa caagcccacc aaagcaaaaa gcccactggc tcacgctagg aaccaaaagg
     5941 cccagcagtg atccagcccc aaaagagatc tcctttgccc cggagattac aatggacgat
     6001 ttcctctatc tttacgatct aggaaggaag ttcgaaggtg aaggtgacga cactatgttc
     6061 accactgata atgagaaggt tagcctcttc aatttcagaa agaatgctga cccacagatg
     6121 gttagagagg cctacgcagc aagtctcatc aagacgatct acccgagtaa caatctccag
     6181 gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaattgc
     6241 atcaagaaca cagagaaaga catatttctc aagatcagaa gtactattcc agtatggacg
     6301 attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc taaaaaggta
     6361 gttcctactg aatctaaggc catgcatgga gtctaagatt caaatcgagg atctaacaga
     6421 actcgccgtc aagactggcg aacagttcat acagagtctt ttacgactca atgacaagaa
     6481 gaaaatcttc gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga
     6541 tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa
     6601 cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga
     6661 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc
     6721 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     6781 ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga
     6841 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     6901 tttggagagg acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca
     6961 aacaacaaac aacattacaa ttacatttac aattatcgat acaatggctt cctctatgct
     7021 ctcttccgct actatggttg cctctccggc tcaggccact atggtcgctc ctttcaacgg
     7081 acttaagtcc tccgctgcct tcccagccac ccgcaaggct aacaacgaca ttacttccat
     7141 cacaagcaac ggcggaagag ttaactgcat gcaggtcatt tatatttctt ctttcacttt
     7201 tttattattc catatgattt ttttcggttc tttcttcgaa tctacataaa ctaatatcat
     7261 tggaaaaatc gaaaaaatag gtgtggcctc cgattggaaa gaagaagttt gagactctct
     7321 cttaccttcc tgaccttacc gattccgaag gtgctcaaga ccagagagaa aactccattg
     7381 atgttgttca gcaaggacaa caaaaaggga accaaggatc aagtgtcgaa aaaagacctc
     7441 aacaacgctt aaccatggat gtctctcctt tcggattgtt ggatcctttg tcaccaatga
     7501 ggacgatgcg acaaatgtta gatactatgg acaggatgtt cgaggacact atgcctgtct
     7561 caggaagaaa cagaggagga agtggagtgt cagagattcg tgcaccgtgg gacatcaaag
     7621 aggaagaaca cgagatcaag atgcgtttcg acatgcctgg actctctaaa gaggacgtca
     7681 aaatctctgt agaagataac gtacttgtga tcaaaggaga gcagaagaag gaggacagtg
     7741 atgattcttg gtctggaaga agcgttagct catatggaac acgacttcag ctcccagaca
     7801 actgtgagaa agacaagatc aaagctgagc tcaagaacgg agtcctcttt atcactatcc
     7861 ctaagaccaa agtcgaacgc aaagtcatcg atgtccagat tcaatcttcg ggcagcgtgc
     7921 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     7981 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     8041 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     8101 acaagtaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg
     8161 caggatccta tccatatgac gttccagatt acgctgctta agcttctcta gctagagtcg
     8221 atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag
     8281 ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     8341 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa
     8401 aatccagatc gctttacgaa ttcccatggg gaggaattcc aatcccacaa aaatctgagc
     8461 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
     8521 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
     8581 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
     8641 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
     8701 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
     8761 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
     8821 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
     8881 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
     8941 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc
     9001 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
     9061 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
     9121 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
     9181 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
     9241 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag
     9301 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     9361 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     9421 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     9481 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     9541 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     9601 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     9661 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     9721 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctactgtata
     9781 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     9841 tttacaatta tcgatacatg gcttctagcc caccgaagaa gaagcggaag gtcagctgga
     9901 aaatggtgag caagggcgag gacctgttca ccggggtggt gcccatcctg gtcgagctgg
     9961 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
    10021 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
    10081 ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc gaccacatga
    10141 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
    10201 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
    10261 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
    10321 acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga
    10381 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
    10441 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
    10501 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
    10561 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
    10621 aagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    10681 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
    10741 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
    10801 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
    10861 gcgcggtgtc atctatgtta ctagatcgac gctcagagag gatgcacatg tgaccgaggg
    10921 acacgaagtg atccgtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
    10981 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
    11041 gttcgtccat ttgtatgtgc cagccgcctt tgcgacgctc accgggctgg ttgccctcgc
    11101 cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg
    11161 tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga
    11221 cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga
    11281 tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga
    11341 aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc
    11401 cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac
    11461 acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc
    11521 cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc
    11581 tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc
    11641 cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc
    11701 ccgctaacgc gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca
    11761 ccccaaaaat ggcagcgctg gccaattccc gagtgcgcgg aacccctatt tgtttatttt
    11821 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    11881 aatattgaaa aaggaagagt atggctaaaa tgagaatatc accggaattg aaaaaactga
    11941 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
    12001 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
    12061 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
    12121 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
    12181 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
    12241 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
    12301 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
    12361 tggattgcga aaactgggaa gaggacactc catttaaaga tccgcgcgag ctgtatgatt
    12421 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
    12481 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
    12541 gggcggacaa gtggtatgac attgccttct gcgtccggtc gctcagggag gatatcgggg
    12601 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
    12661 taaaatatta tattttactg gatgaattgt tttagctgtc agaccaagtt tactcatata
    12721 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    12781 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    12841 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    12901 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    12961 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    13021 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    13081 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    13141 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    13201 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    13261 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    13321 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    13381 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    13441 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggca gatcctagat
    13501 gtggcgcaac gatgccggcg acaagcagga gcgcaccgac ttcttccgca tcaagtgttt
    13561 tggctctcag gccgaggccc acggcaagta tttgggcaag gggtcgctgg tattcgtgca
    13621 gggcaagatt cggaatacca agtacgagaa ggacggccag acggtctacg ggaccgactt
    13681 cattgccgat aaggtggatt atctggacac caaggcacca ggcgggtcaa atcaggaata
    13741 agggcacatt gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac
    13801 gtttgaccgg aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc
    13861 cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg
    13921 ctcgatggtc cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc
    13981 tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt cgtcttgaac aggaggcggc
    14041 aggtttggcg aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa
    14101 aaccgccggc gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa
    14161 acacacgaag cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc
    14221 ggacacgatg cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa
    14281 caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt
    14341 gaagatcacc tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggcagca
    14401 ggtgttggag tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga
    14461 gctttgccag gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg
    14521 cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga
    14581 atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg
    14641 ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt
    14701 catatgggag aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag
    14761 ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga
    14821 ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg
    14881 cagcggcctg gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg
    14941 ccttgtgggg tcagttccgg ctgggggttc agcagcccct gctcggatct gttggaccgg
    15001 acagtagtca tggttgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    15061 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
    15121 agacaactta ataacacatt gcggacgttt ttaatgtact ggggttgaac actctg
//
