LOCUS       sequence-35611-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pKEB31
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..71
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        463..565
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt
       61 cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc accggtgcag
      121 ccttttttct cctgccacat gaagcacttc actgacaccc tcatcagtgc caacatagta
      181 agccagtata cactccgcta gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc
      241 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
      301 aaacaccatc atacactaaa tcagtaagtt ggcagcatca cccgacgcac tttgcgccga
      361 ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga
      421 taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg
      481 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
      541 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc ac
//
