LOCUS       sequence-35612-e         485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pKEB31
FEATURES             Location/Qualifiers
     source          1..485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(112..117,118..135,136..142)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(262..281)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
       61 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      121 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      181 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      241 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      301 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      361 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      421 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      481 catga
//
