LOCUS       sequence-356120-        2312 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encode human IgG1 heavy chain constant region. For cloning variable 
            region and mAb expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2312)
  TITLE     human antibody expression plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AbVec_IGHC
FEATURES             Location/Qualifiers
     source          1..2312
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     270..289
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             join(400..402,403..405,406..1119)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(400..402,403..405,406..1116)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(445..466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(706..725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1053..1074
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      2172..2181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2185..2306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2222..2241)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2276..2295
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgc
      121 ggattccccg tgccaagagt gacgtaagta ccgcctatag agtctatagg cccaccccct
      181 tggcttcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
      241 ttaggtgaca ctatagaata acatccactt tgcctttctc tccacaggtg tccactccca
      301 ggtccaactg cacctcggtt ctatcgattg aattccacca tgggatggtc atgtatcatc
      361 ctttttctag tagcaactgc aaccggtgta cacagctgca tggtgagcaa gggcgaggag
      421 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      481 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      541 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      601 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      661 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      721 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      781 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      841 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      901 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      961 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1021 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1081 gccgggatca ctctcggcat ggacgagctg tacaagtgag cgtcgaccaa gggcccatcg
     1141 gtcttccccc tggcaccctc ctccaagagc acctctgggg gcacagcggc cctgggctgc
     1201 ctggtcaagg actacttccc cgaacctgtg acggtctcgt ggaactcagg cgccctgacc
     1261 agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagcagc
     1321 gtggtgaccg tgccctccag cagcttgggc acccagacct acatctgcaa cgtgaatcac
     1381 aagcccagca acaccaaggt ggacaagaga gttgagccca aatcttgtga caaaactcac
     1441 acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc
     1501 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg
     1561 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg
     1621 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc
     1681 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc
     1741 aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga
     1801 gaaccacagg tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaggtcagc
     1861 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat
     1921 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
     1981 ttcctctata gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca
     2041 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtcc
     2101 ccgggtaaat gagtgcgacg gccggcaagc ccccgctccc cgggctctcg cggtcgtacg
     2161 aggaaagctt ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa
     2221 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2281 ttgtccaaac tcatcaatgt atcttatcat gt
//
