LOCUS       sequence-356121-        1819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encode human Ig kappa chain constant region. For cloning variable 
            region and mAb expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1819)
  TITLE     human antibody expression plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AbVec_IGKC
FEATURES             Location/Qualifiers
     source          1..1819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..27
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        237..255
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     237..254
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     268..287
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             join(398..400,401..403,404..1117)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(398..400,401..403,404..1114)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(443..464)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(704..723)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1051..1072
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1449..1458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1462..1596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1499..1518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1553..1572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
       61 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
      121 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg
      181 gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
      241 aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc cactcccagg
      301 tccaactgca cctcggttct atcgattgaa ttccaccatg ggatggtcat gtatcatcct
      361 ttttctagta gcaactgcaa ccggtgtaca cagctgcatg gtgagcaagg gcgaggagct
      421 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      481 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      541 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      601 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      661 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      721 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      781 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
      841 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
      901 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      961 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1021 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1081 cgggatcact ctcggcatgg acgagctgta caagtgacgt acggtggctg caccatctgt
     1141 cttcatcttc ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct
     1201 gctgaataac ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca
     1261 atcgggtaac tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct
     1321 cagcagcacc ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga
     1381 agtcacccat cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgtta
     1441 gaagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc
     1501 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1561 tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg cgcagcacca
     1621 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     1681 catctgtgga atgtgtgtca gttaggtgtg aaagtcccca ggctcccagc aggcagaagt
     1741 atgcaagcat gcatctcaat tagtcagcac caggtgtgga aagtcccagc tccccagcag
     1801 cagaagtatt gcaaagcat
//
