LOCUS       sequence-356122-        1858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encode human Ig lambda chain constant region. For cloning variable 
            region and mAb expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1858)
  TITLE     human antibody expression plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AbVec_IGLC
FEATURES             Location/Qualifiers
     source          1..1858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        242..260
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     242..259
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     273..292
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             join(400..402,403..405,406..1119)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(400..402,403..405,406..1116)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(445..466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(706..725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1053..1074
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1449..1458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1462..1596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1499..1518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1553..1572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1687..1707)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tatagcagag ctcgtttagt gaccgtcaga tcgcctggag acgccatcca cgctgttttg
       61 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
      121 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc
      181 ccttggcttc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
      241 gatttaggtg acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc
      301 ccaggtccaa ctgcacctcg gttctatcga ttgaattcca ccatgggatg gtcatgtatc
      361 atcctttttc tagtagcaac tgcaaccggt tcttggccaa tggtgagcaa gggcgaggag
      421 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      481 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      541 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      601 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      661 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      721 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      781 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      841 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      901 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      961 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1021 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1081 gccgggatca ctctcggcat ggacgagctg tacaagtgat ggccagccca aggctgcccc
     1141 cactgtcact ctgttcccac cctcgagtga ggagcttcaa gccaacaagg ccacactggt
     1201 gtgtctcata agtgacttct acccgggagc cgtgacagtg gcctggaagg cagatagcag
     1261 ccccgtcaag gcgggagtgg agaccaccac accctccaaa caaagcaaca acaagtacgc
     1321 ggccagcagc tacctgagcc tgacgcctga gcagtggaag tcccacagaa gctacagctg
     1381 ccaggtcacg catgaaggga gcaccgtgga gaagacagtg gcccctacag aatgttcata
     1441 gaagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc
     1501 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1561 tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg cgcagcacca
     1621 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaagaacc
     1681 atctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     1741 gtatgcaagc atgcatctca ttagtcagca ccaggtgtga aagtcccagg ctccccagca
     1801 gcagagtttg caagcatgca tctcattagt cagcacatag tcccgcccct aactcgca
//
