LOCUS       sequence-356149-        4132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non peptide (NP) as negative control fused to EV membrane binging 
            domain (C1C2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Komuro H, Kawai-Harada Y, Aminova S, Pascual N, Malik A, Contag CH, 
            Harada M
  TITLE     Engineering Extracellular Vesicles to Target Pancreatic Tissue In 
            Vivo.
  JOURNAL   Nanotheranostics. 2021 Apr 15;5(4):378-390. doi: 10.7150/ntno.54879.
            eCollection 2021.
  PUBMED    33912378
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.7150/ntno.54879"; journalName: "Nanotheranostics"; date: 
            "2021-04-15- 15"; volume: "5"; issue: "4"; pages: "378-390"
COMMENT     Sequence Label: pcS-NP-C1C2
FEATURES             Location/Qualifiers
     source          1..4132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(102..121)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        293..672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        673..876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     827..847
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     921..940
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        921..939
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1049..1075
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2084..2101
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2124..2141)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2130..2354
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2435..3023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2515..2534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3194..3985,3986..4054))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3817..3836
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(4055..4132,1..27))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
       61 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
      121 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      181 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      241 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      301 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      361 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      421 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      481 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      541 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      601 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      661 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      721 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      781 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      841 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
      901 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt
      961 gtacaaaaaa gcaggcacca tgccgcgccc ccgcctgctg gccgcgctgt gcggcgcgct
     1021 gctctgcgcc cccagcctcc tcgtcgccta tccatatgat gttccagatt atgctggtgg
     1081 aggcggttca ggcggaggtg gctctggcgg tggcggatcg gaatgtgtcg agccactggg
     1141 catggagaat gggaacattg ccaactcaca gatcgccgcc tcatctgtgc gtgtgacctt
     1201 cttgggtttg cagcattggg tcccggagct ggcccgcctg aaccgcgcag gcatggtcaa
     1261 tgcctggaca cccagcagca atgacgataa cccctggatc caggtgaacc tgctgcggag
     1321 gatgtgggta acaggtgtgg tgacgcaggg tgccagccgc ttggccagtc atgagtacct
     1381 gaaggccttc aaggtggcct acagccttaa tggacacgaa ttcgatttca tccatgatgt
     1441 taataaaaaa cacaaggagt ttgtgggtaa ctggaacaaa aacgcggtgc atgtcaacct
     1501 gtttgagacc cctgtggagg ctcagtacgt gagattgtac cccacgagct gccacacggc
     1561 ctgcactctg cgctttgagc tactgggctg tgagctgaac ggatgcgcca atcccctggg
     1621 cctgaagaat aacagcatcc ctgacaagca gatcacggcc tccagcagct acaagacctg
     1681 gggcttgcat ctcttcagct ggaacccctc ctatgcacgg ctggacaagc agggcaactt
     1741 caacgcctgg gttgcgggga gctacggtaa cgatcagtgg ctgcaggtgg acctgggctc
     1801 ctcgaaggag gtgacaggca tcatcaccca gggggcccgt aactttggct ctgtccagtt
     1861 tgtggcatcc tacaaggttg cctacagtaa tgacagtgcg aactggactg agtaccagga
     1921 ccccaggact ggcagcagta agatcttccc tggcaactgg gacaaccact cccacaagaa
     1981 gaacttgttt gagacgccca tcctggctcg ctatgtgcgc atcctgcctg tagcctggca
     2041 caaccgcatc gccctgcgcc tggagctgct gggctgtacc ggtcatcatc accatcacca
     2101 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     2161 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     2221 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     2281 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     2341 ggtgggctct atggggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3061 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3301 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt ta
//
