LOCUS       sequence-356151-        9446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon optimized tardigrade damage suppressing 
            protein Dsup under a CMV promoter; to provide increased radio 
            tolerance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Westover C, Najjar D, Meydan C, Grigorev K, Veling MT, Chang ML, 
            Chin C, Butler D, Afshin EE, Silver PA, Mason CE
  TITLE     Multi-omics Analysis of Dsup Expressing Human Cells Reveals Open 
            Chromatin Architectural Dynamics Underlying Radioprotection
  JOURNAL   bioRxiv 2020.11.10.373571
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020.11.10.373571"
COMMENT     Sequence Label: Dsup pLVX-Puro
FEATURES             Location/Qualifiers
     source          1..9446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2200..2503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2504..2707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2658..2678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     RBS             3098..3106
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4143..4169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4143..4169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4195..4769,4770..4772,4773..4781)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4362..4379)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4589..4608
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4801..5400
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4801..4820)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5297..5317
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5414..6002
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5467..5487)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5885..5896)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6209..6842
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6970..6986)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6970..6986)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6983..7005)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6994..7010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7018..7024,7025..7042,7043..7048))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7063..7084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7201..7218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7372..7960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7452..7471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8131..8922,8923..8991))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8754..8773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8992..9096)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9144..9278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9181..9200)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9235..9254
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9446,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2221 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2281 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2341 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2401 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2461 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2521 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2581 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2641 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgactct actagaggat ctatttccgg tgaattcatg gcctccaccc
     2821 accagagcag caccgagcct tccagcaccg gcaaatccga ggaaacaaag aaggacgcca
     2881 gccaaggttc cggccaagat tccaagaacg tgacagtcac caagggcacc ggctccagcg
     2941 ccactagcgc cgccatcgtg aagaccggag gcagccaagg taaagacagc tccacaaccg
     3001 ccggatcctc cagcacccaa ggtcagaagt tctccaccac ccctacagac cctaagacct
     3061 tttcctccga ccagaaggaa aagtccaagt cccccgctaa ggaggtgcct agcggcggag
     3121 actccaagtc ccaaggtgac accaagagcc agagcgacgc caaaagcagc ggccagagcc
     3181 aaggtcagag caaggactcc ggcaagagct cctccgactc cagcaagagc cacagcgtga
     3241 tcggcgccgt gaaggacgtc gtggccggcg ctaaagatgt ggctggcaaa gccgtggagg
     3301 acgccccctc cattatgcat accgccgtgg acgctgtgaa gaacgccgcc accaccgtca
     3361 aagacgtggc ctccagcgct gccagcacag tggccgagaa agtggtcgac gcctaccaca
     3421 gcgtggtcgg cgataagaca gatgacaaga aggagggcga gcacagcggc gacaaaaagg
     3481 acgacagcaa ggctggaagc ggcagcggcc aaggtggcga taacaagaag agcgagggcg
     3541 agacaagcgg ccaagctgag agctccagcg gcaatgaggg cgccgctccc gctaagggca
     3601 gaggaagggg cagacctccc gctgccgcca aaggagtggc caaaggcgct gctaagggcg
     3661 ccgctgccag caaaggcgcc aaatccggcg ccgagagctc caaaggagga gaacagtcca
     3721 gcggcgacat cgagatggcc gacgccagca gcaagggagg aagcgaccag agagactccg
     3781 ctgctaccgt gggagaaggc ggagccagcg gaagcgaagg cggcgccaaa aagggcagag
     3841 gccgtggcgc cggcaaaaag gccgatgctg gcgatacctc cgccgagcct cccagaagga
     3901 gcagcagact gaccagctcc ggaactggtg ctggatccgc ccccgctgct gctaaaggag
     3961 gcgccaaaag ggccgccagc agcagcagca caccttccaa cgccaagaag caagctactg
     4021 gtggcgctgg caaagctgcc gctaccaagg ccacagccgc caaatccgcc gccagcaagg
     4081 ccccccaaaa tggagccggc gccaagaaga agggcggaaa ggccggcggt cgtaagagga
     4141 aataccccta cgacgtgccc gactacgcct aatctagagc ggccgcggat cccgcccctc
     4201 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4261 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4321 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     4381 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4441 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4501 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4561 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     4621 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4681 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4741 ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc
     4801 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     4861 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     4921 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4981 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5041 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5101 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5161 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5221 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5281 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5341 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctag
     5401 acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5461 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5521 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5581 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5641 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5701 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5761 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     5821 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5881 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5941 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6001 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     6061 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     6121 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6181 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     6241 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     6301 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     6361 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     6421 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     6481 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     6541 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     6601 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     6661 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6721 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6781 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6841 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6901 agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc
     6961 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7021 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7081 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7141 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9121 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
     9181 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9241 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     9301 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     9361 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     9421 aaatatgtac tacaaactta gtagtt
//
