LOCUS       sequence-356152-        4207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p88 as a potential beta-cell (pancreatic tissue) targeting peptide 
            fused to EV membrane binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Komuro H, Kawai-Harada Y, Aminova S, Pascual N, Malik A, Contag CH, 
            Harada M
  TITLE     Engineering Extracellular Vesicles to Target Pancreatic Tissue In 
            Vivo.
  JOURNAL   Nanotheranostics. 2021 Apr 15;5(4):378-390. doi: 10.7150/ntno.54879.
            eCollection 2021.
  PUBMED    33912378
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.7150/ntno.54879"; journalName: "Nanotheranostics"; date: 
            "2021-04-15- 15"; volume: "5"; issue: "4"; pages: "378-390"
COMMENT     Sequence Label: pcS-p88-C1C2
FEATURES             Location/Qualifiers
     source          1..4207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(94..113)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        285..664
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        665..868
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     819..839
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     913..932
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        913..931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1041..1067
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2151..2168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2191..2208)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2197..2421
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2502..3090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2582..2601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3261..4052,4053..4121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3884..3903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(4122..4207,1..19))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
       61 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      121 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
      181 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
      241 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      301 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      361 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      421 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      481 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      541 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      601 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      661 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      721 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      781 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      841 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      901 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gtgtacaaaa
      961 aagcaggcac catgccgcgc ccccgcctgc tggccgcgct gtgcggcgcg ctgctctgcg
     1021 cccccagcct cctcgtcgcc tatccatatg atgttccaga ttatgctctg ccgctgagcc
     1081 gccattatgg cggcggcagc gtgccgtttt atagccatag caacacccat cataccagca
     1141 tgggtggagg cggttcaggc ggaggtggct ctggcggtgg cggatcggaa tgtgtcgagc
     1201 cactgggcat ggagaatggg aacattgcca actcacagat cgccgcctca tctgtgcgtg
     1261 tgaccttctt gggtttgcag cattgggtcc cggagctggc ccgcctgaac cgcgcaggca
     1321 tggtcaatgc ctggacaccc agcagcaatg acgataaccc ctggatccag gtgaacctgc
     1381 tgcggaggat gtgggtaaca ggtgtggtga cgcagggtgc cagccgcttg gccagtcatg
     1441 agtacctgaa ggccttcaag gtggcctaca gccttaatgg acacgaattc gatttcatcc
     1501 atgatgttaa taaaaaacac aaggagtttg tgggtaactg gaacaaaaac gcggtgcatg
     1561 tcaacctgtt tgagacccct gtggaggctc agtacgtgag attgtacccc acgagctgcc
     1621 acacggcctg cactctgcgc tttgagctac tgggctgtga gctgaacgga tgcgccaatc
     1681 ccctgggcct gaagaataac agcatccctg acaagcagat cacggcctcc agcagctaca
     1741 agacctgggg cttgcatctc ttcagctgga acccctccta tgcacggctg gacaagcagg
     1801 gcaacttcaa cgcctgggtt gcggggagct acggtaacga tcagtggctg caggtggacc
     1861 tgggctcctc gaaggaggtg acaggcatca tcacccaggg ggcccgtaac tttggctctg
     1921 tccagtttgt ggcatcctac aaggttgcct acagtaatga cagtgcgaac tggactgagt
     1981 accaggaccc caggactggc agcagtaaga tcttccctgg caactgggac aaccactccc
     2041 acaagaagaa cttgtttgag acgcccatcc tggctcgcta tgtgcgcatc ctgcctgtag
     2101 cctggcacaa ccgcatcgcc ctgcgcctgg agctgctggg ctgtaccggt catcatcacc
     2161 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     2221 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2281 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     2341 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     2401 gggatgcggt gggctctatg gggggataac gcaggaaaga acatgtgagc aaaaggccag
     2461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2941 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3541 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3601 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3661 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3721 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3781 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3841 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3901 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3961 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4021 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4081 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4201 aaaaata
//
