LOCUS       sequence-356153-        4153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pep1 as cell penetrating peptide fused to EV membrane binging domain
            (C1C2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Komuro H, Kawai-Harada Y, Aminova S, Pascual N, Malik A, Contag CH, 
            Harada M
  TITLE     Engineering Extracellular Vesicles to Target Pancreatic Tissue In 
            Vivo.
  JOURNAL   Nanotheranostics. 2021 Apr 15;5(4):378-390. doi: 10.7150/ntno.54879.
            eCollection 2021.
  PUBMED    33912378
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.7150/ntno.54879"; journalName: "Nanotheranostics"; date: 
            "2021-04-15- 15"; volume: "5"; issue: "4"; pages: "378-390"
COMMENT     Sequence Label: pcS-pep1-C1C2
FEATURES             Location/Qualifiers
     source          1..4153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..283
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     234..254
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     328..347
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        328..346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             456..482
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1512..1529
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1552..1569)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1558..1782
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1863..2451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1943..1962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2622..3413,3414..3482))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3245..3264
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3483..3587)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3662..3681)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(3853..4153,1..79)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
       61 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      121 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      181 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      241 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      301 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
      361 ctagcgtgta caaaaaagca ggcaccatgc cgcgcccccg cctgctggcc gcgctgtgcg
      421 gcgcgctgct ctgcgccccc agcctcctcg tcgcctatcc atatgatgtt ccagattatg
      481 ctagccatag cctgctgcat catggtggag gcggttcagg cggaggtggc tctggcggtg
      541 gcggatcgga atgtgtcgag ccactgggca tggagaatgg gaacattgcc aactcacaga
      601 tcgccgcctc atctgtgcgt gtgaccttct tgggtttgca gcattgggtc ccggagctgg
      661 cccgcctgaa ccgcgcaggc atggtcaatg cctggacacc cagcagcaat gacgataacc
      721 cctggatcca ggtgaacctg ctgcggagga tgtgggtaac aggtgtggtg acgcagggtg
      781 ccagccgctt ggccagtcat gagtacctga aggccttcaa ggtggcctac agccttaatg
      841 gacacgaatt cgatttcatc catgatgtta ataaaaaaca caaggagttt gtgggtaact
      901 ggaacaaaaa cgcggtgcat gtcaacctgt ttgagacccc tgtggaggct cagtacgtga
      961 gattgtaccc cacgagctgc cacacggcct gcactctgcg ctttgagcta ctgggctgtg
     1021 agctgaacgg atgcgccaat cccctgggcc tgaagaataa cagcatccct gacaagcaga
     1081 tcacggcctc cagcagctac aagacctggg gcttgcatct cttcagctgg aacccctcct
     1141 atgcacggct ggacaagcag ggcaacttca acgcctgggt tgcggggagc tacggtaacg
     1201 atcagtggct gcaggtggac ctgggctcct cgaaggaggt gacaggcatc atcacccagg
     1261 gggcccgtaa ctttggctct gtccagtttg tggcatccta caaggttgcc tacagtaatg
     1321 acagtgcgaa ctggactgag taccaggacc ccaggactgg cagcagtaag atcttccctg
     1381 gcaactggga caaccactcc cacaagaaga acttgtttga gacgcccatc ctggctcgct
     1441 atgtgcgcat cctgcctgta gcctggcaca accgcatcgc cctgcgcctg gagctgctgg
     1501 gctgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
     1561 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     1621 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     1681 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     1741 aagacaatag caggcatgct ggggatgcgg tgggctctat ggggggataa cgcaggaaag
     1801 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1861 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1921 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1981 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2041 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2101 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2161 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2221 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2281 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2341 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2401 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2461 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2521 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2581 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2641 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2701 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2761 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2821 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2881 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2941 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3001 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3061 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3121 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3181 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3241 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3301 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3361 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3421 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3481 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3541 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3601 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     3661 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     3721 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     3781 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     3841 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     3901 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     3961 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     4021 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     4081 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     4141 aatggcccgc ctg
//
