LOCUS       sequence-356181-       10349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DEAA UPF1 inserted with GFP tagged on the N-terminus used to stably 
            integrate into cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: pci-EGFP-UPF1-DEAA
FEATURES             Location/Qualifiers
     source          1..10349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        113..492
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     647..667
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          863..995
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     978..997
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1040..1058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1074..1083
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1080..1082,1083..1085,1086..1796)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1080..1082,1083..1085,1086..1796)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1125..1146)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1386..1405)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1733..1754
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5235..5356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5246..5265)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5300..5319
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5542..5997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6114..6471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6114..6305
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6137..6157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6322..6457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6522..7316
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6576..6595)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7186..7205
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7380..7428
                     /note="synthetic polyadenylation signal"
     promoter        complement(7504..7522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7536..7552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7536..7552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7549..7571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7560..7576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7584..7590,7591..7608,7609..7614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7629..7650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7767..7784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7938..8526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8018..8037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8697..9488,9489..9557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9320..9339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9558..9662)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9689..10144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     10270..10292
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10284..10301
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10285..10301
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
       61 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
      121 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      181 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      241 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      301 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      361 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      421 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
      481 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
      541 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      601 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
      661 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaattga
      721 tctaccatga gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct
      781 tctgacacaa cagtctcgaa cttaagctgc agtgactctc ttaaggtagc cttgcagaag
      841 ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca
      901 atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg
      961 tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag
     1021 ctcttaaggc tagagtactt aatacgactc actataggct agcgctaccg gtcgccacca
     1081 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1141 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1201 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1261 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1321 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1381 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1441 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1501 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1561 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1621 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1681 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1741 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagctta
     1801 gcgtggaggc gtacgggccc agctcgcaga ctctcacttt cctggacacg gaggaggccg
     1861 agctgcttgg cgccgacaca cagggctccg agttcgagtt caccgacttt actcttccta
     1921 gccagacgca gacgcccccc ggcggccccg gcggcccggg cggtggcggc gcgggaggcc
     1981 cgggcggcgc gggcgcgggc gctgcggcgg gacagctcga cgcgcaggtt gggcccgaag
     2041 gcatcctgca gaacggggct gtggacgaca gtgtagccaa gaccagccag ttgttggctg
     2101 agttgaactt cgaggaagat gaagaagaca cctattacac gaaggacctc cccatacacg
     2161 cctgcagtta ctgtggaata cacgatcctg cctgcgtggt ttactgtaat accagcaaga
     2221 agtggttctg caacggacgt ggaaatactt ctggcagcca cattgtaaat caccttgtga
     2281 gggcaaaatg caaagaggtg accctgcaca aggacgggcc cctgggggag acagtcctgg
     2341 agtgctacaa ctgcggctgt cgcaacgtct tcctcctcgg cttcatcccg gccaaagctg
     2401 actcagtggt ggtgctgctg tgcaggcagc cctgtgccag ccagagcagc ctcaaggaca
     2461 tcaactggga cagctcgcag tggcagccgc tgatccagga ccgctgcttc ctgtcctggc
     2521 tggtcaagat cccctccgag caggagcagc tgcgggcacg ccagatcacg gcacagcaga
     2581 tcaacaagct ggaggagctg tggaaggaaa acccttctgc cacgctggag gacctggaga
     2641 agccgggggt ggacgaggag ccgcagcatg tcctcctgcg gtacgaggac gcctaccagt
     2701 accagaacat attcgggccc ctggtcaagc tggaggccga ctacgacaag aagctgaagg
     2761 agtcccagac tcaagataac atcactgtca ggtgggacct gggccttaac aagaagagaa
     2821 tcgcctactt cactttgccc aagactgact ctgacatgcg gctcatgcag ggggatgaga
     2881 tatgcctgcg gtacaaaggg gaccttgcgc ccctgtggaa agggatcggc cacgtcatca
     2941 aggtccctga taattatggc gatgagatcg ccattgagct gcggagcagc gtgggtgcac
     3001 ctgtggaggt gactcacaac ttccaggtgg attttgtgtg gaagtcgacc tcctttgaca
     3061 ggatgcagag cgcattgaaa acgtttgccg tggatgagac ctcggtgtct ggctacatct
     3121 accacaagct gttgggccac gaggtggagg acgtaatcat caagtgccag ctgcccaagc
     3181 gcttcacggc gcagggcctc cccgacctca accactccca ggtttatgcc gtgaagactg
     3241 tgctgcaaag accactgagc ctgatccagg gcccgccagg cacggggaag acggtgacgt
     3301 cggccaccat cgtctaccac ctggcccggc aaggcaacgg gccggtgctg gtgtgtgctc
     3361 cgagcaacat cgccgtggac cagctaacgg agaagatcca ccagacgggg ctaaaggtcg
     3421 tgcgcctctg cgccaagagc cgtgaggcca tcgactcccc ggtgtctttt ctggccctgc
     3481 acaaccagat caggaacatg gacagcatgc ctgagctgca gaagctgcag cagctgaaag
     3541 acgagactgg ggagctgtcg tctgccgacg agaagcggta ccgggccttg aagcgcaccg
     3601 cagagagaga gctgctgatg aacgcagatg tcatctgctg cacatgtgtg ggcgccggtg
     3661 acccgaggct ggccaagatg cagttccgct ccattttaat cgccgcaagc acccaggcca
     3721 ccgagccgga gtgcatggtt cccgtggtcc tcggggccaa gcagctgatc cttgtaggcg
     3781 accactgcca gctgggccca gtggtgatgt gcaagaaggc ggccaaggcc gggctgtcac
     3841 agtcgctctt cgagcgcctg gtggtgctgg gcatccggcc catccgcctg caggtccagt
     3901 accggatgca ccctgcactc agcgccttcc catccaacat cttctacgag ggctccctcc
     3961 agaatggtgt cactgcagcg gatcgtgtga agaagggatt tgacttccag tggccccaac
     4021 ccgataaacc gatgttcttc tacgtgaccc agggccaaga ggagattgcc agctcgggca
     4081 cctcctacct gaacaggacc gaggctgcga acgtggagaa gatcaccacg aagttgctga
     4141 aggcaggcgc caagccggac cagattggca tcatcacgcc ctacgagggc cagcgctcct
     4201 acctggtgca gtacatgcag ttcagcggct ccctgcacac caagctctac caggaggtgg
     4261 agatcgccag tgtggacgcc tttcagggac gcgagaagga cttcatcatc ctgtcctgtg
     4321 tgcgggccaa cgagcaccaa ggcattggct ttttaaatga ccccaggcgt ctgaacgtgg
     4381 ccctgaccag agcaaggtat ggcgtcatca ttgtgggcaa cccgaaggca ctatcaaagc
     4441 agccgctctg gaaccacctg ctgaactact ataaggagca gaaggtgctg gtggaggggc
     4501 cgctcaacaa cctgcgtgag agcctcatgc agttcagcaa gccacggaag ctggtcaaca
     4561 ctatcaaccc gggagcccgc ttcatgacca cagccatgta tgatgcccgg gaggccatca
     4621 tcccaggctc cgtctatgat cggagcagcc agggccggcc ttccagcatg tacttccaga
     4681 cccatgacca gattggcatg atcagtgccg gccctagcca cgtggctgcc atgaacattc
     4741 ccatcccctt caacctggtc atgccaccca tgccaccgcc tggctatttt ggacaagcca
     4801 acgggcctgc tgcagggcga ggcaccccga aaggcaagac tggtcgtggg ggacgccaga
     4861 agaaccgctt tgggcttcct ggacccagcc agactaacct ccccaacagc caagccagcc
     4921 aggatgtggc gtcacagccc ttctctcagg gcgccctgac gcagggctac atctccatga
     4981 gccagccttc ccagatgagc cagcccggcc tctcccagcc ggagctgtcc caggacagtt
     5041 accttggtga cgagtttaaa tcacaaatcg acgtggcgct ctcacaggac tccacgtacc
     5101 agggagagcg ggcttaccag catggcgggg tgacggggct gtcccagtat taaaaggtgg
     5161 cggcggaaga gctaaagagt cgacccgggc ggccgcttcc ctttagtgag ggttaatgct
     5221 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     5281 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     5341 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     5401 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg
     5461 atcgatccgg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5521 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5581 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5641 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5701 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5761 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5821 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5881 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5941 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct
     6001 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc
     6061 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
     6121 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgat
     6481 tcttctgaca caacagtctc gaacttaagg ctagagccac catgattgaa caagatggat
     6541 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6601 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6661 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6721 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6781 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6841 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6901 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6961 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7021 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     7081 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     7141 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     7201 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     7261 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     7321 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gatggccgca
     7381 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga
     7441 taaggatccc ctaggctttt gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa
     7501 ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     7561 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7621 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7681 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8581 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8641 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8701 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8761 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8821 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8881 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8941 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9001 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9061 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9121 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9181 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9241 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9301 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9361 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9421 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9481 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9541 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9601 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9661 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     9721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9961 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10021 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10081 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    10141 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10201 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10261 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg atctatacat
    10321 tgaatcaata ttggcaatta gccatatta
//
