LOCUS       sequence-356185-        4455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produce cRNA that expresses a human MCU-EMRE fusion protein in 
            Xenopus oocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Rodriguez MX, Van Keuren AM, Tsai MF
  TITLE     Quantitative assays to measure the transport activity of the 
            mitochondrial calcium uniporter in cell lines or Xenopus oocytes.
  JOURNAL   STAR Protoc. 2021 Nov 23;2(4):100979. doi: 
            10.1016/j.xpro.2021.100979. eCollection 2021 Dec 17.
  PUBMED    34877549
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2021-11-23"; volume: "2(4):100979. doi"; pages: " 
            10.1016/j.xpro.2021.100979. eCollection 2021 Dec 17"
COMMENT     Sequence Label: hME-pOX
FEATURES             Location/Qualifiers
     source          1..4455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(49..67)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(77..94)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(77..93)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(86..108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      235..690
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(322..341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     532..553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        716..820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(821..889,890..1681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1039..1058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1852..2440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2341..2360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2594..2611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2728..2749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2764..2769,2770..2787,2788..2794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2802..2818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2807..2829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2826..2842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2826..2842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2861..2881
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2863..2881
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           2903..2945
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           4297..4422
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 cccccccccc ccccccgcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt
       61 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      121 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      181 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
      241 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      301 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      361 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      421 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
      481 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      541 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      601 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
      661 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
      721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      781 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      961 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1021 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1201 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     1321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     1381 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     1441 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     1501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     1561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1681 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1741 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1801 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1861 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1921 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1981 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2041 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2101 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2161 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2221 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2281 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2341 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2401 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2461 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2521 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2581 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     2641 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2701 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2761 ggctttacac tttatgctcc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     2821 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     2881 ggaacaaaag ctgggtaccc ttcttgttct ttttgcagaa gctcagaata aacgctcaac
     2941 tttggcagag ctagcgttta aacttaagct tggtaccgag ctcggatcca ctagtccagt
     3001 gtggtggaat tcgaaatggc gcagaacctg gagggggaat ggcagaatct gggagccgtg
     3061 tactgctcca cagtggtccc ttctgacgat gtcactgtgg tctatcagaa cggactgccc
     3121 gtgatcagtg tccgcctgcc ttcacggaga gagcgatgtc agtttaccct gaagcctatt
     3181 agcgactccg tgggagtctt cctgcggcag ctgcaggagg aagacagagg catcgatagg
     3241 gtggccatct actctccaga cggcgtgaga gtcgccgcta gtactgggat cgatctgctg
     3301 ctgctggacg acttcaagct ggtcatcaac gacctgacct accacgtccg cccccctaaa
     3361 cgagatctgc tgagccatga gaacgcagcc actctgaatg acgtgaagac cctggtccag
     3421 cagctgtata ccacactgtg catcgagcag caccagctga acaaggagcg ggaactgatt
     3481 gagagactgg aagatctgaa ggagcagctg gcacccctgg agaaagtgag gatcgaaatt
     3541 tcccgcaagg ccgaaaaacg aactaccctg gtgctgtggg gaggactggc atacatggct
     3601 acccagtttg gcatcctggc taggctgaca tggtgggagt attcctggga catcatggaa
     3661 cccgtgacct actttattac atatgggtct gcaatggcca tgtacgccta tttcgtgatg
     3721 acaaggcagg agtacgtcta tcctgaagct agggatcgcc agtacctgct gttctttcac
     3781 aagggagcaa agaaatctcg cttcgacctg gagaagtata accagctgaa agatgctatc
     3841 gcacaggccg aaatggacct gaagcgactg cgggatcctc tgcaggtgca tctgccactg
     3901 cggcagatcg gcgagaaaga cgtgagcgcc gcatggtctg gaagtggccg gagcctggtg
     3961 ccatcaagaa gcgtgatcgt cacaaggagc ggggccattc tgccaaagcc cgtgaaaatg
     4021 agcttcggac tgctgagagt gttttccatc gtcattccct tcctgtacgt gggcactctg
     4081 atcagtaaaa attttgccgc tctgctggag gaacatgata ttttcgtgcc agaggacgat
     4141 gacgatgacg atacagaaac tagccaggtg gctcccgcat gagatatcga attcctgcag
     4201 cccgggggat ccactagtct cgagtctaga agtttctgct acgagaacga ggttgctctt
     4261 acgtctaagt gataatcgag gatctggtta ccactaaacc agcctcaaga acacccgaat
     4321 ggagtctcta agctacataa taccaactta cactttacaa aatgttgtcc cccaaaatgt
     4381 agccattcgt atctgctcct aataaaaaga aagtttcttc acattctaaa aaaaaaaaaa
     4441 aaaaaaaaaa aaaaa
//
