LOCUS       sequence-356283-        8156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encoding a dominant negative form of CEBP under inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Kudo T, Zhao ML, Kovary K, LaGory L, Covert MW, Teruel MN
  TITLE     A dynamic HIF1-alpha-PPAR-gamma circuit controls a paradoxical 
            adipocyte regulatory landscape
  JOURNAL   bioRxiv (2022)
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2022)"
COMMENT     Sequence Label: dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro
FEATURES             Location/Qualifiers
     source          1..8156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..27
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    51..75
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             84..107
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             111..143
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     protein_bind    complement(419..443)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             461..478
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        488..998
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     917..935
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1008..1718
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             1719..1772
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1773..2390,2391..2396,2397..2432,2433..2435,2436..2471,
                     2472..2474,2475..2510,2511..2519)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(2559..2579)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2977..2988)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3193..3426
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3498..3619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3535..3554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3589..3608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3659..3794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3794..3865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3866..3934,3935..4726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4084..4103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4897..5485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5386..5405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5639..5656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5773..5794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5809..5814,5815..5832,5833..5839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5847..5863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5852..5874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5871..5887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5871..5887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5906..5926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5908..5926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5954..6180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6181..6361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6408..6533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7026..7259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7444..7488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7701..7818
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(7872..8156,1..30)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    7880..7898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7916..7934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7951..7969
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7987..8005
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8023..8041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8058..8076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8094..8112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcgcc tggagaattg gctagcatca acaagtttgt
       61 acaaaaaagc aggctccacc atggactaca aggacgacga tgacaagcat atggctagca
      121 tgactggtgg acagcaaatg ggtcgggatc ctgacctgga acaacgtgct gaggaactgg
      181 cccgtgaaaa cgaagagctg gaaaaagagg ccgaagagct ggagcaggaa aacgctgaac
      241 tcgagcagaa ggtgttggag ttgaccagtg acaatgaccg cctgcgcaag cgggtggaac
      301 agctgagccg tgaactggac acgctgcggg gtatcttccg ccagctgcct gagagctcct
      361 tggtcaaggc catgggcaac tgcgcgtgag cctaagcggc cgcactcgag atatctagac
      421 ccagctttct tgtacaaagt ggtttagtaa tgaaccggtc caccaccacc accaccacta
      481 aggatccggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
      541 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
      601 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
      661 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
      721 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
      781 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
      841 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
      901 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
      961 gctccctcgt tgaccgaatc accgacctct ctccccagca attcaccatg gtgtctaagg
     1021 gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt catggaaggt tcggtcaacg
     1081 gccaccaatt caaatgcaca ggtgaaggag aaggcaatcc gtacatggga actcaaacca
     1141 tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt cttgccacgt
     1201 cgttcatgta tggcagccgt acttttatca agtacccgaa aggcattcct gatttcttta
     1261 aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa gatggtggag
     1321 tcgtcaccgt catgcaggac accagccttg aggatggctg tctcgtttac cacgtccaag
     1381 tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaagacc aagggttggg
     1441 agcctaatac agagatgatg tatccagcag atggtggtct gaggggatac actcatatgg
     1501 cactgaaagt tgatggtggt ggccatctgt cttgctcttt cgtaacaact tacaggtcaa
     1561 aaaagaccgt cgggaacatc aagatgcccg gtatccatgc cgttgatcac cgcctggaaa
     1621 ggttagagga aagtgacaat gaaatgttcg tagtacaacg cgaacacgca gttgccaagt
     1681 tcgccgggct tggtggtggg atggacgagc tgtacaagga aggtagaggt tctctcctca
     1741 cttgtggtga tgttgaagaa aaccctggtc caatgtctag actggacaag agcaaagtca
     1801 taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac
     1861 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     1921 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     1981 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
     2041 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     2101 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     2161 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     2221 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     2281 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     2341 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
     2401 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     2461 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac
     2521 taagtaagga tcgatccaag atatcgtatt cttaactatg ttgctccttt tacgctatgt
     2581 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2641 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2701 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2761 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2821 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2881 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     2941 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3001 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3061 acgagtcgga tctccctttg ggccgcctcc ccgcctgttt cgcctcggcg tccggactag
     3121 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3181 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     3241 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3421 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3481 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3541 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3601 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3661 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3721 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3781 cttttttgga ggcctttcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     5761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     5821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     5881 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     5941 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     6001 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     6061 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     6121 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     6181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6361 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     6421 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     6481 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     6541 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6601 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     6661 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     6721 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     6781 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     6841 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     6901 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     6961 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     7021 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     7081 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     7141 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     7201 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     7261 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     7321 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     7381 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     7441 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     7501 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     7561 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     7621 ccattatcgt ttcagaccca cctcccaacc ccgaggggac aattctcgac ctcgagacaa
     7681 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     7741 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     7801 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcga
     7861 atcgatatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     7921 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     7981 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     8041 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     8101 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagc
//
