LOCUS       sequence-356285-       10244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encoding stblHIF under inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Kudo T, Zhao ML, Kovary K, LaGory L, Covert MW, Teruel MN
  TITLE     A dynamic HIF1-alpha-PPAR-gamma circuit controls a paradoxical 
            adipocyte regulatory landscape
  JOURNAL   bioRxiv (2022)
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2022)"
COMMENT     Sequence Label: tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro
FEATURES             Location/Qualifiers
     source          1..10244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..69
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2557..2568)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(2663..2687)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2705..2722
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2732..3242
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3161..3179
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3252..3848
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3252..3271)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3748..3768
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3849..3902
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3903..4520,4521..4526,4527..4562,4563..4565,4566..4601,
                     4602..4604,4605..4640,4641..4649)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4689..4709)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5107..5118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5323..5556
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5628..5749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5665..5684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5719..5738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5789..5924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5924..5995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5996..6064,6065..6856)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6214..6233)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7027..7615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7516..7535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7769..7786
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7903..7924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7939..7944,7945..7962,7963..7969)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7977..7993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7982..8004
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8001..8017
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8001..8017
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8036..8056
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8038..8056
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8084..8310
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8311..8491
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8538..8663
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9156..9389
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9574..9618
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9831..9948
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10002..10244,1..72)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    10010..10028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10046..10064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10081..10099
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10117..10135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10153..10171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10188..10206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10224..10242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
       61 cgtcagatcg cctggagaat tggctagcat caacaagttt gtacaaaaaa gcaggctcca
      121 ccatggaggg cgccggcggc gagaacgaga agaaaaagat gagttctgaa cgtcgaaaag
      181 aaaagtctag agatgcagca agatctcggc gaagcaaaga gtctgaagtt ttttatgagc
      241 ttgctcatca gttgccactt ccccacaatg tgagctcaca tcttgataaa gcttctgtta
      301 tgaggctcac catcagttat ttacgtgtga gaaaacttct ggatgccggt ggtctagaca
      361 gtgaagatga gatgaaggca cagatggact gtttttatct gaaagcccta gatggctttg
      421 tgatggtgct aacagatgac ggcgacatgg tttacatttc tgataacgtg aacaaataca
      481 tggggttaac tcagtttgaa ctaactggac acagtgtgtt tgattttact cattcatgtg
      541 accatgagga aatgagagaa atgcttacac acagaaatgg cccagtgaga aaagggaaag
      601 aactaaacac acagcggagc ttttttctca gaatgaagtg caccctaaca agccggggga
      661 ggacgatgaa catcaagtca gcaacgtgga aggtgcttca ctgcacgggc catattcatg
      721 tctatgatac caacagtaac caacctcagt gtgggtacaa gaaaccaccc atgacgtgct
      781 tggtgctgat ttgtgaaccc attcctcatc cgtcaaatat tgaaattcct ttagacagca
      841 agacatttct cagtcgacac agcctcgata tgaaattttc ttactgtgat gaaagaatta
      901 ctgagttgat gggttatgag ccggaagaac ttttgggccg ctcaatttat gaatattatc
      961 atgctttgga ttctgatcat ctgaccaaaa ctcaccatga tatgtttact aaaggacaag
     1021 tcaccacagg acagtacagg atgcttgcca aaagaggtgg atatgtctgg gttgaaactc
     1081 aagcaactgt catatataat acgaagaact cccagccaca gtgcattgtg tgtgtgaatt
     1141 atgttgtaag tggtattatt cagcacgact tgattttctc ccttcaacaa acagaatctg
     1201 tgctcaaacc agttgaatct tcagataaga agatgactca gctgttcacc aaagttgaat
     1261 cagaggctac aagctgcctt tttgataagc ttaagaagga gcctgatgct ctcactctgc
     1321 tggctgcagc tgccggcgac accatcatct ctctggattt tggcagcgat gacacagaaa
     1381 ctgaagatca acaacttgaa gatgttccat tatataatga tgtaatgttt ccctcttcta
     1441 atgaaaaatt aaatataaac ctggcaatgt ctcctttacc ttcatcggaa actccaaagc
     1501 cacttcgaag tagtgctgat cctgcactga atcaagaggt tgcattaaaa ttagaatcaa
     1561 gtccagagtc actgggactt tcttttacca tgccccagat tcaagatcag ccagcaagtc
     1621 cttctgatgg aagcactaga caaagttcac ctgagagact tcttcaggaa aacgtaaaca
     1681 ctcctaactt ttcccagcct aacagtccca gtgaatattg ctttgatgtg gatagcgata
     1741 tggtcaatgt attcaagttg gaactggtgg aaaaactgtt tgctgaagac acagaggcaa
     1801 agaatccatt ttcaactcag gacactgatt tagacttgga gatgctggct ggctatatcc
     1861 caatggatga tgatttccag ttacgttcct ttgatcagtt gtcaccatta gagagcaatt
     1921 ctccaagccc tccaagtatg agcacagtta ctgggttcca gcagacccag ttacagaaac
     1981 ctaccatcac tgccactgcc accacaactg ccaccactga tgaatcaaaa acagagacga
     2041 aggacaataa agaagatatt aaaatactga ttgcatctcc atcttctacc caagtacctc
     2101 aagaaacgac cactgctaag gcatcagcat acagtggcac tcacagtcgg acagcctcac
     2161 cagacagagc aggaaagaga gtcatagaac agacagacaa agctcatcca aggagcctta
     2221 acctgtctgc cactttgaat caaagaaata ctgttcctga ggaagaatta aacccaaaga
     2281 caatagcttc gcagaatgct cagaggaagc gaaaaatgga acatgatggc tccctttttc
     2341 aagcagcagg aattggaaca ttattgcagc aaccgggtga ctgtgcacct actatgtcac
     2401 tttcctggaa acgagtgaaa ggattcatat ctagtgaaca gaatggaacg gagcaaaaga
     2461 ctattatttt aataccctcc gatttagcat gcagactgct ggggcagtca atggatgaga
     2521 gtggattacc acagctgacc agttacgatt gtgaagttgc agctcccata caaggcagca
     2581 gaaacctact gcagggtgaa gaattactca gagctttgga tcaagttaac taagcctaag
     2641 cggccgcact cgagatatct agacccagct ttcttgtaca aagtggttta gtaatgaacc
     2701 ggtccaccac caccaccacc actaaggatc cggggttggg gttgcgcctt ttccaaggca
     2761 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     2821 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     2881 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     2941 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     3001 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     3061 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     3121 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     3181 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     3241 agcaattcac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     3301 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     3361 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     3421 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     3481 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     3541 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     3601 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     3661 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     3721 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     3781 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     3841 ccggtgccga aggtagaggt tctctcctca cttgtggtga tgttgaagaa aaccctggtc
     3901 caatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     3961 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     4021 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     4081 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     4141 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     4201 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     4261 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     4321 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     4381 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     4441 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     4501 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     4561 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     4621 attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt
     4681 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4741 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4801 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4861 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4921 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4981 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     5041 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5101 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5161 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5221 ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg
     5281 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5341 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5401 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5461 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5521 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     5581 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5641 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5701 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     5761 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5821 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5881 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctttcaa atatgtatcc
     5941 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6001 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6061 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6121 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6181 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6241 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6301 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6361 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6421 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6481 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6541 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6601 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6661 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6721 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6781 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6841 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6901 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6961 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7621 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7681 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7741 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7801 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     7861 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7921 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7981 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     8041 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     8101 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     8161 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     8221 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     8281 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     8341 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8401 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8461 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     8521 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     8581 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8641 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8701 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8761 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8821 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8881 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8941 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     9001 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     9061 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     9121 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     9181 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     9241 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     9301 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     9361 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     9421 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     9481 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     9541 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9601 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9661 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9721 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9781 ccgaggggac aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
     9841 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     9901 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     9961 agggacagca gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt
    10021 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac
    10081 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
    10141 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga
    10201 tagagaacgt atgtcgagtt tactccctat cagtgataga gaac
//
