LOCUS       sequence-356313-        2754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and
            6HIS tags from pET24 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Sudan R, Fernandes S, Srivastava N, Pedicone C, Meyer ST, Chisholm 
            JD, Engelman RW, Kerr WG
  TITLE     LRBA Deficiency Can Lead to Lethal Colitis That Is Diminished by 
            SHIP1 Agonism.
  JOURNAL   Front Immunol. 2022 May 4;13:830961. doi: 10.3389/fimmu.2022.830961.
            eCollection 2022.
  PUBMED    35603158
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2022-05-4"; volume: "13:830961. doi"; pages: " 
            10.3389/fimmu.2022.830961. eCollection 2022"
COMMENT     Sequence Label: pET24TEV-tSHIP1
FEATURES             Location/Qualifiers
     source          1..2754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..33
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             1639..1647
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2704..2724
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2734..2751
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctagca tgactggtgg acagcaaatg ggtcgggatc ctatggtccc ctgctggaac
       61 catggcaaca tcacccgctc caaggcggag gagctgcttt ccaggacagg caaggacggg
      121 agcttcctcg tgcgtgccag cgagtccatc tcccgggcat acgcgctctg cgtgctgtat
      181 cggaattgcg tttacactta cagaattctg cccaatgaag atgataaatt cactgttcag
      241 gcatccgaag gcgtctccat gaggttcttc accaagctgg accagctcat cgagttttac
      301 aagaaggaaa acatggggct ggtgacccat ctgcaatacc ctgtgccgct ggaggaagag
      361 gacacaggcg acgaccctga ggaggacaca gtagaaagtg tcgtgtctcc acccgagctg
      421 cccccaagaa acatcccgct gactgccagc tcctgtgagg ccaaggaggt tcctttttca
      481 aacgagaatc cccgagcgac cgagaccagc cggccgagcc tctccgagac attgttccag
      541 cgactgcaaa gcatggacac cagtgggctt ccagaagagc atcttaaggc catccaagat
      601 tatttaagca ctcagctcgc ccaggactct gaatttgtga agacagggtc cagcagtctt
      661 cctcacctga agaaactgac cacactgctc tgcaaggagc tctatggaga agtcatccgg
      721 accctcccat ccctggagtc tctgcagagg ttatttgacc agcagctctc cccgggcctc
      781 cgtccacgtc ctcaggttcc tggtgaggcc aatcccatca acatggtgtc caagctcagc
      841 caactgacaa gcctgttgtc atccattgaa gacaaggtca aggccttgct gcacgagggt
      901 cctgagtctc cgcaccggcc ctcccttatc cctccagtca cctttgaggt gaaggcagag
      961 tctctgggga ttcctcagaa aatgcagctc aaagtcgacg ttgagtctgg gaaactgatc
     1021 attaagaagt ccaaggatgg ttctgaggac aagttctaca gccacaagaa aatcctgcag
     1081 ctcattaagt cacagaaatt tctgaataag ttggtgatct tggtggaaac agagaaggag
     1141 aagatcctgc ggaaggaata tgtttttgct gactccaaaa agagagaagg cttctgccag
     1201 ctcctgcagc agatgaagaa caagcactca gagcagccgg agcccgacat gatcaccatc
     1261 ttcatcggca cctggaacat gggtaacgcc ccccctccca agaagatcac gtcctggttt
     1321 ctctccaagg ggcagggaaa gacgcgggac gactctgcgg actacatccc ccatgacatt
     1381 tacgtgatcg gcacccaaga ggaccccctg agtgagaagg agtggctgga gatcctcaaa
     1441 cactccctgc aagaaatcac cagtgtgact tttaaaacag tcgccatcca cacgctctgg
     1501 aacatccgca tcgtggtgct ggccaagcct gagcacgaga accggatcag ccacatctgt
     1561 actgacaacg tgaagacagg cattgcaaac acactgggga acaagggagc cgtgggggtg
     1621 tcgttcatgt tcaatggaac ctccttaggg ttcgtcaaca gccacttgac ttcaggaagt
     1681 gaaaagaaac tcaggcgaaa ccaaaactat atgaacattc tccggttcct ggccctgggc
     1741 gacaagaagc tgagtccctt taacatcact caccgcttca cgcacctctt ctggtttggg
     1801 gatcttaact accgtgtgga tctgcctacc tgggaggcag aaaccatcat ccagaaaatc
     1861 aagcagcagc agtacgcaga cctcctgtcc cacgaccagc tgctcacaga gaggagggag
     1921 cagaaggtct tcctacactt cgaggaggaa gaaatcacgt ttgccccaac ctaccgtttt
     1981 gagagactga ctcgggacaa atacgcctac accaagcaga aagcgacagg gatgaagtac
     2041 aacttgcctt cctggtgtga ccgagtcctc tggaagtctt atcccctggt gcacgtggtg
     2101 tgtcagtctt atggcagtac cagcgacatc atgacgagtg accacagccc tgtctttgcc
     2161 acatttgagg caggagtcac ttcccagttt gtctccaaga acggtcccgg gactgttgac
     2221 agccaaggac agattgagtt tctcaggtgc tatgccacat tgaagaccaa gtcccagacc
     2281 aaattctacc tggagttcca ctcgagctgc ttggagagtt ttgtcaagag tcaggaagga
     2341 gaaaatgaag aaggaagtga gggggagctg gtggtgaagt ttggtgagac tcttccaaag
     2401 ctgaagccca ttatctctga ccctgagtac ctgctagacc agcacatcct catcagcatc
     2461 aagtcctctg acagcgacga atcctatggc gagggctgca ttgcccttcg gttagaggcc
     2521 acagaaacgc agctgcccat ctacacgcct ctcacccacc atggggagtt gacaggccac
     2581 ttccaggggg agatcaagct gcagacctct cagggcaaga cgagggagaa gctctatgac
     2641 tttgtgaaga cggagcgtga tgaatccagt gggccaaaga ccctgaagag caagcttcta
     2701 gaggaaaacc tgtattttca gggctccctc gagcaccacc accaccacca ctga
//
