LOCUS       sequence-356316-        3012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV 
            and 6HIS tags from pET24 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Sudan R, Fernandes S, Srivastava N, Pedicone C, Meyer ST, Chisholm 
            JD, Engelman RW, Kerr WG
  TITLE     LRBA Deficiency Can Lead to Lethal Colitis That Is Diminished by 
            SHIP1 Agonism.
  JOURNAL   Front Immunol. 2022 May 4;13:830961. doi: 10.3389/fimmu.2022.830961.
            eCollection 2022.
  PUBMED    35603158
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2022-05-4"; volume: "13:830961. doi"; pages: " 
            10.3389/fimmu.2022.830961. eCollection 2022"
COMMENT     Sequence Label: pET24TEV-tSHIP2
FEATURES             Location/Qualifiers
     source          1..3012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..33
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(1931..1942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2962..2982
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2992..3009
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctagca tgactggtgg acagcaaatg ggtcgggatc ctatggcccc ctcctggtac
       61 caccgcgacc tgagccgggc ggccgcggag gagctgctgg cccgggcggg ccgcgatggc
      121 agcttcctgg tccgagacag cgagagcgtg gcgggggcct tcgcgctctg cgtcctgtat
      181 cagaagcatg tgcacacgta tcgcattctg cctgatggag aagatttctt ggctgtgcag
      241 acctcgcagg gtgtgcctgt gcgccgcttc cagaccctgg gtgagctcat cggcctgtac
      301 gcccagccca accagggcct tgtgtgcgcc ctgcttcttc ctgtagaggg tgagcgagag
      361 ccggacccac cggatgaccg ggatgcctca gatggggagg atgagaagcc cccgctgccc
      421 ccgcgctctg gctccaccag catttctgcc cccactgggc ccagcagtcc cctgccagct
      481 cctgagactc ccacagctcc agctgctgag agtgctccca atgggctgag caccgtctcg
      541 cacgactacc tgaaaggcag ctatgggctg gacctggaag ctgtgagggg tggagccagc
      601 cacctgcccc acctcacccg taccctcgct acctcatgcc ggaggctgca cagtgaggtg
      661 gacaaggtcc tgtcaggcct ggagatcctg tccaaggtgt ttgaccagca gagctcgccc
      721 atggtgaccc gccttttgca gcagcagaac ctgccacaga caggggagca ggaactagag
      781 agcctggtgc tgaagctgtc agtgctaaag gacttcctgt caggcatcca gaagaaggcc
      841 ctgaaggccc tacaggacat gagctccaca gcacccccag ctccgcagcc atccacacgt
      901 aaggccaaga ccatccccgt gcaggccttt gaggtgaagc tagatgtgac cctgggtgac
      961 ctgaccaaga ttgggaagtc acagaagttc acgctgagcg tggatgtgga gggtgggcgg
     1021 ctggtgctgc tgcggagaca gcgggactcc caggaggact ggaccacctt cacgcacgac
     1081 cgcatccgcc agctcattaa gtcccagcgt gtccagaaca agctgggtgt tgtgtttgag
     1141 aaggagaagg accggactca gcgcaaggac ttcatctttg tcagtgcccg gaagcgggag
     1201 gccttctgcc agctgttgca gctcatgaag aacaagcact ccaagcagga cgagcccgac
     1261 atgatctcag tcttcatagg cacctggaac atgggaagtg taccacctcc aaaaaacgtg
     1321 acatcctggt tcacatcgaa gggtctgggg aagaccctgg acgaggtcac agtgaccata
     1381 ccccatgaca tctatgtctt tgggacccag gagaactcag tgggcgaccg cgagtggctg
     1441 gacctactgc gcgggggcct caaggagctt acggatctgg attaccgccc gattgccatg
     1501 caatcactgt ggaatatcaa ggtggcagtg ctggtcaagc cagagcacga gaaccgtatc
     1561 agccatgtca gtacgtccag tgtgaagact ggcatcgcca acaccctggg gaacaagggg
     1621 gctgtgggcg tctccttcat gtttaatggc acctcatttg gctttgtgaa ttgtcacctc
     1681 acctcgggaa atgagaagac ggctcggagg aaccaaaact acttggacat cctgcggctg
     1741 ctctcgctgg gcgaccggca gctcaatgcc tttgacatct ctctgcgttt cacacacctc
     1801 ttctggtttg gggacctcaa ctaccgcctg gacatggata tccaggagat cctgaactac
     1861 atcagcagga aagagtttga gcccctcctc agggtggacc agctcaacct ggagcgggag
     1921 aagcacaagg tcttccttcg attcagtgag gaggagatct ccttcccacc cacctaccgc
     1981 tatgagcggg gttcccggga cacatatgcc tggcacaagc agaagccaac tggggtccgg
     2041 accaatgtgc cctcatggtg tgaccggatt ctgtggaaat cctaccctga aactcacatc
     2101 atctgcaatt cttatggttg cactgatgac atcgtcacca gcgaccattc ccccgtgttt
     2161 gggacatttg aggttggagt tacctcccag ttcatctcca agaaagggct ctcaaagact
     2221 tcagaccagg cctacattga gtttgagagc atcgaggcca ttgtgaagac agccagccgc
     2281 accaagttct tcatcgagtt ctactctacc tgcctggagg aatacaagaa gagctttgag
     2341 aatgatgccc agagcagtga caacatcaac ttcctcaaag tgcagtggtc ttcacgccag
     2401 ctgcccacgc tcaaaccaat tctggctgat atcgagtacc tgcaggacca gcacctcctg
     2461 ctcacagtca agtccatgga tggctatgaa tcctatgggg agtgtgtggt tgcactcaaa
     2521 tccatgatcg gcagcacggc ccaacagttc ctgaccttcc tatcccaccg tggcgaggag
     2581 acaggcaata tcagaggctc catgaaggtg cgggtgccca cggagcgcct gggcacccgt
     2641 gagcggctct acgagtggat cagcattgat aaggatgagg caggagcaaa gagcaaagcc
     2701 ccctctgtgt cccgagggag ccaggagccc aggtcaggga gccgcaagcc agccttcaca
     2761 gaggcctcct gcccgctctc caggttattt gaagaaccag agaaaccgcc accaacgggg
     2821 aggcccccag ccccaccccg agcagctccc cgggaggagc ccttgacccc caggttgaag
     2881 ccagagggag ctcctgaacc agaaggggtg gcggcccccc cacccaagaa cagcttcaat
     2941 aaccctgcca agcttctaga ggaaaacctg tattttcagg gctccctcga gcaccaccac
     3001 caccaccact ga
//
