LOCUS       sequence-356322-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sfGFP reporter under the control of Mutant E. coli thiB TPP 
            Riboswitch under the control of a Constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Berman KE, Steans R, Hertz LM, Lucks JB
  TITLE     A transient intermediate RNA structure underlies the regulatory 
            function of the E. coli thiB TPP translational riboswitch.
  JOURNAL   RNA. 2023 Nov;29(11):1658-1672. doi: 10.1261/rna.079427.122. Epub 
            2023 Jul 7.
  PUBMED    37419663
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.079427.122"; journalName: "RNA"; date: "2023-11"; 
            volume: "29"; issue: "11"; pages: "1658-1672"
COMMENT     Sequence Label: JBL6402
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        115..149
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(322..350)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     943..963
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 accccggatt taggtttgta attctcatgt tttgacagct tatcatcgaa taagcttccg
       61 atggcgcgcc gagaggcttt acactttatg cttccggctg aattctaaag atctttgaca
      121 gctagctcag tcctaggtat aatactagtc cgttctcaac ggggtcccac gcgtacgcgt
      181 gcgctgagaa aatacccgtc gaacctgatc cggataacgc cggcgaaggg atttgaggct
      241 ccttctcaag tcctttgcca ctcttttttg aggtgcaaag tgttaaaaaa aatgagcaaa
      301 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      361 gggcacaaat tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc
      421 cttaaattta tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact
      481 ctgacctatg gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt
      541 ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac
      601 gggacctaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc
      661 gagttaaagg gtattgattt taaagaagat ggaaacattc ttggacacaa actcgagtac
      721 aactttaact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct
      781 aacttcaaaa ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa
      841 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
      901 caatctgtcc tttcaaagat cccaacgaaa gcgtgaccac atggtccttc ttgagtttgt
      961 aactgctgct gggattacac atggcatgga tgagcttcta
//
