LOCUS       sequence-356458-         315 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  VP16 activation domain coupled to catalytically inactive dCas9 for 
            localization; labeled with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K
  TITLE     Transcription activation is enhanced by multivalent interactions 
            independent of phase separation.
  JOURNAL   Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017.
  PUBMED    35537448
REFERENCE   2  (bases 1 to 315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017."
COMMENT     Sequence Label: NLS-dCas9-HA-NLS-GFP-VP16
FEATURES             Location/Qualifiers
     source          1..315
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..45
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agggggcgtc gactgggtac ccctcctaag aagaagagga aggttgcccc caccatggat
       61 aaaaagaaga gaaaggcccc cccgaccgat gtcagcctgg gggacgagct ccacttagac
      121 ggcgaggacg tggcgatggc gcatgccgac gcgctagacg atttcgatct ggacatgttg
      181 ggggacgggg attccccggg tccgggattt accccccacg actccgcccc ctacggcgct
      241 ctggatatgg ccgacttcga gtttgagcag atgtttaccg atgcccctct agacgggggg
      301 atccaccgga tctag
//
