LOCUS       sequence-35650-e        2082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2305 (=HL 716 + pHL 765)
FEATURES             Location/Qualifiers
     source          1..2082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      36..122
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(286..874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(366..385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(962..1056)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1086..1877,1878..1946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1709..1728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1947..2051)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgggggat cccatggtac gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga
       61 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      121 atccgccgcc ctagacctag gcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      481 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      721 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga ctagtgcttg
      961 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     1021 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgta aacttggtct
     1081 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1141 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1201 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1261 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1321 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1381 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1441 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1501 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1561 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1621 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1681 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1741 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1801 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1861 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1921 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1981 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2041 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
