LOCUS       sequence-356515-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic PHR domain coupled to GFP and Rta activation domain; 
            binds to CIBN upon blue light exposure.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Trojanowski J, Frank L, Rademacher A, Mucke N, Grigaitis P, Rippe K
  TITLE     Transcription activation is enhanced by multivalent interactions 
            independent of phase separation.
  JOURNAL   Mol Cell. 2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017.
  PUBMED    35537448
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2022 May 4. pii: S1097-2765(22)00328-8. doi: 
            10.1016/j.molcel.2022.04.017."
COMMENT     Sequence Label: PHR-GFP-Rta
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..570
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 cgggattcca gggaagggat gtttttgccg aagcctgagg ccggctccgc tattagtgac
       61 gtgtttgagg gccgcgaggt gtgccagcca aaacgaatcc ggccatttca tcctccagga
      121 agtccatggg ccaaccgccc actccccgcc agcctcgcac caacaccaac cggtccagta
      181 catgagccag tcgggtcact gaccccggca ccagtccctc agccactgga tccagcgccc
      241 gcagtgactc ccgaggccag tcacctgttg gaggatcccg atgaagagac gagccaggct
      301 gtcaaagccc ttcgggagat ggccgatact gtgattcccc agaaggaaga ggctgcaatc
      361 tgtggccaaa tggacctttc ccatccgccc ccaaggggcc atctggatga gctgacaacc
      421 acacttgagt ccatgaccga ggatctgaac ctggactcac ccctgacccc ggaattgaac
      481 gagattctgg ataccttcct gaacgacgag tgcctcttgc atgccatgca tatcagcaca
      541 ggactgtcca tcttcgacac atctctgttt
//
