LOCUS       sequence-356526-       10096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral transduction of 3FLAG-TFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Park H, Hundley FV, Yu Q, Overmyer KA, Brademan DR, Serrano L, Paulo
            JA, Paoli JC, Swarup S, Coon JJ, Gygi SP, Wade Harper J
  TITLE     Spatial snapshots of amyloid precursor protein intramembrane 
            processing via early endosome proteomics.
  JOURNAL   Nat Commun. 2022 Oct 16;13(1):6112. doi: 10.1038/s41467-022-33881-x.
  PUBMED    36245040
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-16"; volume: "13(1):6112. doi"; pages: " 
            10.1038/s41467-022-33881-x"
COMMENT     Sequence Label: pHAGE-3FLAG-TFR1
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2835..2844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2844..2867
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2871..2894
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2898..2921
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2937..2961
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3906..3917)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5251..5275)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5288..5856
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5306..5858
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5450..5467)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5677..5696
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5870..6469
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5870..5889)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6366..6386
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6486..7074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6539..6559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6957..6968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7149..7382
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7501..7519)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7587..7691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7692..7760,7761..8552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7910..7929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8723..9311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9212..9231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9465..9482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9722..9743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9758..9763,9764..9781,9782..9788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9796..9812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9801..9823
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9820..9836
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9820..9836
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9886..9905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct
     2821 ctcgaggttg atctgccacc atggactaca aggatgacga tgacaagggc gattacaagg
     2881 atgacgacga taagggagat tacaaggatg acgacgataa ggctcgaggt tatcaaacaa
     2941 gtttgtacaa aaaagcaggc tccaccatga tggatcaagc tagatcagca ttctctaact
     3001 tgtttggtgg agaaccattg tcatataccc ggttcagcct ggctcggcaa gtagatggcg
     3061 ataacagtca tgtggagatg aaacttgctg tagatgaaga agaaaatgct gacaataaca
     3121 caaaggccaa tgtcacaaaa ccaaaaaggt gtagtggaag tatctgctat gggactattg
     3181 ctgtgatcgt ctttttcttg attggattta tgattggcta cttgggctat tgtaaagggg
     3241 tagaaccaaa aactgagtgt gagagactgg caggaaccga gtctccagtg agggaggagc
     3301 caggagagga cttccctgca gcacgtcgct tatattggga tgacctgaag agaaagttgt
     3361 cggagaaact ggacagcaca gacttcaccg gcaccatcaa gctgctgaat gaaaattcat
     3421 atgtccctcg tgaggctgga tctcaaaaag atgaaaatct tgcgttgtat gttgaaaatc
     3481 aatttcgtga atttaaactc agcaaagtct ggcgtgatca acattttgtt aagattcagg
     3541 tcaaagacag cgctcaaaac tcggtgatca tagttgataa gaacggtaga cttgtttacc
     3601 tggtggagaa tcctgggggt tatgtggcgt atagtaaggc tgcaacagtt actggtaaac
     3661 tggtccatgc taattttggt actaaaaaag attttgagga tttatacact cctgtgaatg
     3721 gatctatagt gattgtcaga gcagggaaaa tcacctttgc agaaaaggtt gcaaatgctg
     3781 aaagcttaaa tgcaattggt gtgttgatat acatggacca gactaaattt cccattgtta
     3841 acgcagaact ttcattcttt ggacatgctc atctggggac aggtgaccct tacacacctg
     3901 gattcccttc cttcaatcac actcagtttc caccatctcg gtcatcagga ttgcctaata
     3961 tacctgtcca gacaatctcc agagctgctg cagaaaagct gtttgggaat atggaaggag
     4021 actgtccctc tgactggaaa acagactcta catgtaggat ggtaacctca gaaagcaaga
     4081 atgtgaagct cactgtgagc aatgtgctga aagagataaa aattcttaac atctttggag
     4141 ttattaaagg ctttgtagaa ccagatcact atgttgtagt tggggcccag agagatgcat
     4201 ggggccctgg agctgcaaaa tccggtgtag gcacagctct cctattgaaa cttgcccaga
     4261 tgttctcaga tatggtctta aaagatgggt ttcagcccag cagaagcatt atctttgcca
     4321 gttggagtgc tggagacttt ggatcggttg gtgccactga atggctagag ggataccttt
     4381 cgtccctgca tttaaaggct ttcacttata ttaatctgga taaagcggtt cttggtacca
     4441 gcaacttcaa ggtttctgcc agcccactgt tgtatacgct tattgagaaa acaatgcaaa
     4501 atgtgaagca tccggttact gggcaatttc tatatcagga cagcaactgg gccagcaaag
     4561 ttgagaaact cactttagac aatgctgctt tccctttcct tgcatattct ggaatcccag
     4621 cagtttcttt ctgtttttgc gaggacacag attatcctta tttgggtacc accatggaca
     4681 cctataagga actgattgag aggattcctg agttgaacaa agtggcacga gcagctgcag
     4741 aggtcgctgg tcagttcgtg attaaactaa cccatgatgt tgaattgaac ctggactatg
     4801 agaggtacaa cagccaactg ctttcatttg tgagggatct gaaccaatac agagcagaca
     4861 taaaggaaat gggcctgagt ttacagtggc tgtattctgc tcgtggagac ttcttccgtg
     4921 ctacttccag actaacaaca gatttcggga atgctgagaa aacagacaga tttgtcatga
     4981 agaaactcaa tgatcgtgtc atgagagtgg agtatcactt cctctctccc tacgtatctc
     5041 caaaagagtc tcctttccga catgtcttct ggggctccgg ctctcacacg ctgccagctt
     5101 tactggagaa cttgaaactg cgtaaacaaa ataacggtgc ttttaatgaa acgctgttca
     5161 gaaaccagtt ggctctagct acttggacta ttcagggagc tgcaaatgcc ctctctggtg
     5221 acgtttggga cattgacaat gagttttaag acccagcttt cttgtacaaa gtggttcgat
     5281 aacgaattcc gccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5341 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5401 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5461 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5521 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5581 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5641 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5701 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5761 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5821 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tgaccgagta
     5881 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5941 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     6001 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6061 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6121 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6181 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6241 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6301 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6361 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6421 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat gcatcgatgg
     6481 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6541 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6601 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6661 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6721 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6781 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6841 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6901 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6961 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7021 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga
     7081 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7141 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7201 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7261 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7321 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7381 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7441 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg
     7501 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7561 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7621 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7921 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8221 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8401 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9361 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9421 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9481 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9541 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9661 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     9721 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     9781 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
     9841 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9901 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     9961 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    10021 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    10081 ctacaaactt agtagt
//
