LOCUS       sequence-356527-        9328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha 
            protein, both are contolled by tetracycline mediated TDH promter, 
            test protein expression and stability..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Dong C, Chen SJ, Melnykov A, Weirich S, Sun K, Jeltsch A, Varshavsky
            A, Min J
  TITLE     Recognition of nonproline N-terminal residues by the Pro/N-degron 
            pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):14158-14167. doi: 
            10.1073/pnas.2007085117. Epub 2020 Jun 8.
  PUBMED    32513738
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2007085117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-06-23- 23"; volume: "117"; issue: "25"; pages: 
            "14158-14167"
COMMENT     Sequence Label: pCSJ982
FEATURES             Location/Qualifiers
     source          1..9328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        317..504
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             505..1167
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1427..1882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1564..1585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1776..1795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2008..2030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2022..2039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2046..2065
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2046..2064
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2095..2761
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             3036..3263
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             4314..4379
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4430..4619
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        4648..5314
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             5592..5615
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5625..6185
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             6192..6218
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      6228..6475
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6510..6526)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6511..6527)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(6555..6573)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6594..6610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6594..6610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6607..6629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6618..6634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6642..6648,6649..6666,6667..6672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6687..6708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6825..6842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6996..7584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7076..7095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7755..8546,8547..8615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8378..8397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8616..8720)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8750..9253
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9301..9319
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat
      421 aatgaattat acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag
      481 caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca
      541 aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac
      601 tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg
      661 attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat
      721 tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc
      781 actgaagact gcgggattgc tctcggtcaa gcttttaaag aggccctagg ggccgtgcgt
      841 ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc cagagcggtg
      901 gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta
      961 ggagatctct cttgcgagat gatcccgcat tttcttgaaa gctttgcaga ggctagcaga
     1021 attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg
     1081 ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac caacgatgtt
     1141 ccctccacca aaggtgttct tatgtagtga caccgattat ttaaagctgc agcatacgat
     1201 atatatacat gtgtatatat gtatacctat gaatgtcagt aagtatgtat acgaacagta
     1261 tgatactgaa gatgacaagg taatgcatca ttctatacgt gtcattctga acgaggcgcg
     1321 ctttcctttt ttctttttgc tttttctttt tttttctctt gaactcgacg gatctatgcg
     1381 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     1441 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     1501 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     1561 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     1621 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1681 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1741 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1801 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1861 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggcgac tttatcatta
     2101 tcaatactgc catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta
     2161 tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg
     2221 cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc
     2281 cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc
     2341 accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact
     2401 acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac
     2461 ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc
     2521 ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga
     2581 aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat
     2641 tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc
     2701 ttttttttag ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa
     2761 aatcgtacgg gcctaaaaca taccagatcg ccacccgcgc tttaatctgg agaggtgaag
     2821 aatacgacca cctaggcctt aagaaccggt aaaacatacc agatcgccac ccgcgcttta
     2881 atctggagag gtgaagaata cgaccaccta ccggtcaaca acaacaacaa caacaacaac
     2941 aactcgaggc ctaaaacata ccagatcgcc acccgcgctt taatctggag aggtgaagaa
     3001 tacgaccacc taggcctcga gaacataggc gcgccatgca gatctttgtg aagaccctca
     3061 ctggcaaaac catcaccctt gaggtcgagc ccagtgacac cattgagaat gtcaaagcca
     3121 aaattcaaga caaggagggt atcccacctg accagcagcg tctgatattt gccggcaaac
     3181 agctggagga tggccgcact ctctcagact acaacatcca gaaagagtcc accctgcacc
     3241 tggtgttgcg cctccgcggt ggtatgccag gtttgtggaa tggaccaaga agagactcta
     3301 ccgaagggtt tgataccgat atcatcactc ttcctagatt cataatcgag caccagaagc
     3361 aatttaagaa cgctactggt gatttcacat tagtactgaa tgccttgcaa ttcgcgttca
     3421 aatttgtatc tcacaccatc agacgtgctg aattggttaa cttggttggg ttagcaggcg
     3481 cttccaactt cactggtgac cagcaaaaga agttggacgt tctaggtgat gaaatattta
     3541 tcaatgccat gagggctagt gggatcatca aggtccttgt atctgaagaa caggaagact
     3601 tgatcgtttt tcccacaaac acgggctcat acgcagtgtg ttgtgatcct attgatggct
     3661 cctcaaattt ggacgccggt gtctccgttg gaactatcgc gtctatattc agactgctac
     3721 cagactcatc aggtactata aacgacgtac tgagatgtgg taaagaaatg gtagccgctt
     3781 gctatgccat gtacggatcc tctacgcatc tagtattgac attgggtgat ggagttgatg
     3841 ggtttacctt agacacaaac ttgggcgaat tcatcttgac tcatcctaac ttaagaattc
     3901 cgcctcaaaa ggccatctac tcaattaatg aaggtaacac cctctactgg aacgagacta
     3961 taagaacatt tattgagaaa gtcaaacaac cccaagcaga caacaacaac aagcctttct
     4021 cggctaggta tgttggatcc atggttgctg atgttcacag gacgtttctt tacggtggcc
     4081 ttttcgcata cccttgcgac aagaagagcc ccaacggaaa actgaggttg ctttatgagg
     4141 ccttcccaat ggctttctta atggaacaag cagggggaaa agcggtcaac gatcgcggag
     4201 agagaatctt ggatttggtg ccaagtcata tccatgacaa atcttctatt tggttgggtt
     4261 cttcaggtga aattgacaaa tttttagacc atattggcaa gtcacagaga tccgactata
     4321 aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac gatgacaagt
     4381 aagcggccgc attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa
     4441 ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta
     4501 tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg
     4561 tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg
     4621 ctttaatttg cggccggccc gactttatca ttatcaatac tgccatttca aagaatacgt
     4681 aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag ccttttaatt
     4741 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcgt
     4801 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt
     4861 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcaccaacc
     4921 atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac aaacaggcaa
     4981 aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga tgacacaagg
     5041 caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc ttctgctctc
     5101 tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat tattccccta
     5161 cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct
     5221 taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa acaccaagaa
     5281 cttagtttcg aataaacaca cataaacaaa caaaatcgta cgggcctaaa acataccaga
     5341 tcgccacccg cgctttaatc tggagaggtg aagaatacga ccacctaggc cttaagaacc
     5401 ggtaaaacat accagatcgc cacccgcgct ttaatctgga gaggtgaaga atacgaccac
     5461 ctaccggtca acaacaacaa caacaacaac aacaactcga ggcctaaaac ataccagatc
     5521 gccacccgcg ctttaatctg gagaggtgaa gaatacgacc acctaggcct cgagaacata
     5581 tcgaattcat ggactacaag gacgacgatg acaagggtgg gtccatggtt cgaccattga
     5641 actgcatcgt cgccgtgtcc caaaatatgg ggattggcaa gaacggagac ctaccctggc
     5701 ctccgctcag gaacgagttc aagtacttcc aaagaatgac cacaacctct tcagtggaag
     5761 gtaaacagaa tctggtgatt atgggtagga aaacctggtt ctccattcct gagaagaatc
     5821 gacctttaaa ggacagaatt aatatagttc tcagtagaga actcaaagaa ccaccacgag
     5881 gcgctcattt tcttgccaaa agtttggatg atgccttaag acttattgaa caaccggaat
     5941 tggcaagtaa agtagacatg gtttggatag tcggaggcag ttctgtttac caggaagcca
     6001 tgaatcaacc aggccacctc agactctttg tgacaaggat catgcaggaa tttgaaagtg
     6061 acacgttttt cccagaaatt gatttgggga aatataaact tctcccagaa tacccaggcg
     6121 tcctctctga ggtccaggag gaaaaaggca tcaagtataa gtttgaagtc tacgagaaga
     6181 aagacggtac ctacccatac gatgtcccag attacgccta actcgagtca tgtaattagt
     6241 tatgtcacgc ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt
     6301 tagacaacct gaagtctagg tccctattta tttttttata gttatgttag tattaagaac
     6361 gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat
     6421 tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccc
     6481 gggctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt
     6541 accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     6601 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6661 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6721 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7741 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7801 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7861 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7921 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7981 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8041 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8101 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8161 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8221 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8281 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8341 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8401 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8461 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8521 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8581 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8641 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8701 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgg atcgcttgcc
     8761 tgtaacttac acgcgcctcg tatcttttaa tgatggaata atttgggaat ttactctgtg
     8821 tttatttatt tttatgtttt gtatttggat tttagaaagt aaataaagaa ggtagaagag
     8881 ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa atttcaacaa aaagcgtact
     8941 ttacatatat atttattaga caagaaaagc agattaaata gatatacatt cgattaacga
     9001 taagtaaaat gtaaaatcac aggattttcg tgtgtggtct tctacacaga caagatgaaa
     9061 caattcggca ttaatacctg agagcaggaa gagcaagata aaaggtagta tttgttggcg
     9121 atccccctag agtcttttac atcttcggaa aacaaaaact attttttctt taatttcttt
     9181 ttttactttc tatttttaat ttatatattt atattaaaaa atttaaatta taattatttt
     9241 tatagcacgt gatgaaaagg accctaagaa accattatta tcatgacatt aacctataaa
     9301 aataggcgta tcacgaggcc ctttcgtc
//
