LOCUS       sequence-356539-        9489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1-1148.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot1_1-1148_I
FEATURES             Location/Qualifiers
     source          1..9489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6740..6781
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6791..6808
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6831..6848)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6881..7015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6905..6924)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6959..6978
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7616..8204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7696..7715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8375..9166,9167..9235))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8998..9017
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9236..9340)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9408..9426
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttgaattc attaattaag atgaacgtag agagccaact gaagacaccg
     3301 ccaacgcaca ttcgaaatct ggtcaagcac gtgaacaagc gaaattttaa tgagagcagc
     3361 gagcaaataa atcagttcgt taaagagcac ggcctggaag cggatcgcag cagcctgcgc
     3421 cacctgttct ccgtgatcaa cttcagcgat ctcgtgccat cagttacggt ccagctgcag
     3481 gcgaagctcc tgggcatcca gttggagcgt cagcttcaca gttcatcgtt tgtgtccaac
     3541 atctgctacg cttttgacca gttcttcgcc agcaaccaga agtccctgaa gcctgttgcg
     3601 gtagcggatc tcgttggcca ggtcgcccga ctgaccggca tcaacaaggt ttgtgagtgc
     3661 gtctttgcct tggccttgac ccactcctcg tacacagaac tcaaacattc tgccaggaac
     3721 aacctaaaag gtagcctcag cgaacttata gactcttact tgggtaataa aggcaccggc
     3781 ccagccgaca gcggtctccg ggagatctcc ttcgatctac tgcagtattt gctgtgctgc
     3841 cttagcgagt acgtacaacc gcaggtggaa gcacagtttt tgatcaagct tcgtgaggag
     3901 tttccgcgcc aggcggtacc gctggttcta gcgccgttcc tttacggctc gacgacggcg
     3961 acgattgcgg gagcaggtgc cagcgaaacg gatgcggagg ccgaagcgac gactaatagc
     4021 aacagctcca gtttcgaagc agatgctttg aacgaggtgg gaatcgagga tatttatgac
     4081 catttaagcg agataatatt caccaaccag ggcaaaaata atattatgga cacatcttgg
     4141 attaatctaa tccttgaaat cggttatgaa tttacatcga gcgttgaaga gtgtaagaat
     4201 catttgtgtt cccgcgagcg agaacgcgct gagctccagt ccaaagatgt cgccaagatc
     4261 gtgggtctta tgtgccgacg gcactcctcc ctgctcgatt gtaatgtgaa tctaccgacg
     4321 ccggcgaact tctggcctgg tcagggacag ggcggtggca gcaacagcag tggctcttcg
     4381 cagacacaaa tcactccgca gcaacagaac ccgggcagca gtaacaacaa cgatggcagc
     4441 gatggcaatt cgtccagtga taagaaggac aagaaggaga cgacggaggc cacgcaaact
     4501 tggaagccag atgtctttgt gcaggcgctc aaggaggtgg tgcctcagct caactggaag
     4561 gacgtgtgca tggaacttga tcatcccgag ttcgtgctga aggatcgcat cggtttggag
     4621 cttttgctca ccatccttcg gctggccact ggctctaaca tatttccaca tccagaatgc
     4681 atttaccgtc actgggcaaa cacggaaggt cagctgtcgc ttatcgcgac aatgctgaag
     4741 aacccggatc tcttttcctt cgccgacttt gtatttagcc agccagcgtt ggatgtgctt
     4801 aagacagctc cggatgcgga caataaggag atctcgtcct ggaagtcgct tcacctggtg
     4861 gaggtgctgc tctccattgc ggacaaggga tactatacgc aggtccatga gctattcaag
     4921 ttcccagcgc agaactgtcc cgatgtgcta tttttggcct tattgaacac aagtccgcct
     4981 atgacgccac tgcgtcagga tctgtttaac caactgatac caacgttcct tggcaaccat
     5041 cccaactcga acgtgatctt ggccagcgcc tggagctcga acagcttcca acttcgttcc
     5101 aatatcatga atgctatgtc cgagtggtat ctccgaggca atgagttcga tcaggtgaaa
     5161 ctgtcgcgca tcctggatct tgcccaggat ttaaaagcgc tatcagcgct gctcaacgcc
     5221 cgttcatttt tattcattat cgacctggct tgccttgcct ctcggcgcga gtacctgaag
     5281 ctggaaaagt ggcttaccga taaaattcgc gaacatggag agccttttat gcaggctata
     5341 atcaaggttc tgcaccgccg ctgcccgcag gttatcaacg ccaaagtgcc agaggatcag
     5401 ctgcctccca agcaggcgca gcttttgccc gagacagtta caaccatgat caactgtctg
     5461 caaacgtgca tcaataactg catgcagccg gagatggtcg aagtcatcat gcaaatgacc
     5521 gcgaacgtgg cgattatggc taacatggcg cgtgcccaac agcagcagca gccaggattg
     5581 gttccgccgc ctccaccaac aattctgcgc ggccatcgtg gtatggatct gcctggaggc
     5641 atagtccctc cgccaccgca gcaaccgttc tccggaaacc tcaacgcgca gatgtttgga
     5701 ccaggaatgg atccgctaac gaatatgtcc aacaacttag ccggcctcaa cctcagcggc
     5761 ccgaacggag catttaattt tggaaacatg ctaacttctc catcgcgtct gatgactccg
     5821 ggagccaatc cctatccgct gaacctcatg cagatgccgc aggctccgcc accacccaac
     5881 gtaggaaacc taggccgcat gctgccaggg ggtccacaac agcagactcc aacgccgact
     5941 ccaacggccc ccaatccaaa caaccctgtg atggccgatc tccagatacc tgtgtcaaag
     6001 gaagttgaag acgaggtcaa ctcatacttt cagcgcatct acaaccacca gccgaatcca
     6061 acgctgtcca tcgatgaagt gctggacatt ttgcaaagat ttaaagagtc aagtaaccgg
     6121 cgcgagcagg aagtcttttt gtgcatgctg cgcaatctgt tcgaggaata tcgctttttc
     6181 tgtcaatacc cagagaaaga gctacagatt actgcgcagc tatttggtgg cattattgat
     6241 cgtaacctgg tgcccacttt tgttgcactg ggcctttctc tgcgctgtgt cttggatgcc
     6301 ctccgcaagc ccgatggatc taagctttac tatttcggtg tgacggcact ggacagattc
     6361 agaactcgat tgcacaccta caacaaatac tgcgagcata tccgctccat tcctcacttc
     6421 tctgacttcc cgccgcacct tattcagtat gtggaatacg gaatgcacgg ccaggagccg
     6481 ccgccgcaga agttgattgg actcagcaat acgattcctt cggcgatatc tagcggacca
     6541 ggaacggaac caatttacag gaatagctcc atgttaggta atatgcccgc tgccaccccg
     6601 ggatcgggac cgaaatcaaa cgctgctgta tcgcatgcca cgagaatgaa gtccatcgcc
     6661 aatgccacca atatcgacac acttttggtg gccaaccagg aagagaaagt gacttgatct
     6721 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     6781 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     6841 gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata
     6901 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6961 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7021 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     7081 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga
     7141 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     7201 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     7261 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     7321 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     7381 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     7441 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     7501 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     7561 agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8041 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8341 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8401 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8461 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8521 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8581 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8641 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     8701 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8761 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8821 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8881 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8941 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     9001 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9061 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9121 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9181 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9241 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9301 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9361 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     9421 cgaggccctt tcgtcttcaa gaattaattc tatattctaa aaacacaaat gatacttcta
     9481 aaaaaaaat
//
