LOCUS       sequence-356576-        9026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses zsGreen fused to a miR-211-5p sponge and mCherry in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   McNeal AS, Belote RL, Zeng H, Urquijo M, Barker K, Torres R, Curtin 
            M, Shain AH, Andtbacka RH, Holmen S, Lum DH, McCalmont TH, 
            VanBrocklin MW, Grossman D, Wei ML, Lang UE, Judson-Torres RL
  TITLE     BRAF(V600E) induces reversible mitotic arrest in human melanocytes 
            via microrna-mediated suppression of AURKB.
  JOURNAL   Elife. 2021 Nov 23;10. pii: 70385. doi: 10.7554/eLife.70385.
  PUBMED    34812139
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-11-23"; volume: "10. pii: 70385. doi"; pages: " 
            10.7554/eLife.70385"
COMMENT     Sequence Label: pLVX-anti-MIR211-5p
FEATURES             Location/Qualifiers
     source          1..9026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             55..688
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    735..860
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1357..1590
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1775..1819
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2082..2197
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2255..2558
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2559..2762
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2713..2733
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2759..2783
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2897..2906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2903..3598
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     promoter        3800..4299
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3835..3858)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4232..4251
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4318..4327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4324..5034
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4469..4487)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4737..4756
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5048..5636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5101..5121)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5519..5530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5843..6476
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6605..6621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6605..6621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6618..6640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6629..6645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6653..6659,6660..6677,6678..6683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6698..6719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6836..6853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7007..7595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7087..7106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7766..8557,8558..8626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8389..8408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8627..8731)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8779..8913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8816..8835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8870..8889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
       61 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
      121 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
      181 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
      241 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
      301 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
      361 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
      421 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
      481 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
      541 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      601 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      661 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
      721 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
      781 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
      841 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
      901 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
      961 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     1021 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     1081 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     1141 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     1201 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1261 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1321 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1381 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1441 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1501 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1561 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1621 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1681 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1741 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1801 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1861 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1921 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1981 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     2041 cagatccatt cgattagtga acggatctcg acggtatcgc ctttaaaaga aaagggggga
     2101 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2161 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2221 gagatccagt ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc
     2281 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     2341 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     2401 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2461 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2521 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2581 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2641 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2701 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2761 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2821 gaagacaccg actctactag aggatcgcta gcgctgaagc ttagatctcg agaattcgcg
     2881 gccgctccgg acgcgtgcca ccatggccca gtccaagcac ggcctgacca aggagatgac
     2941 catgaagtac cgcatggagg gctgcgtgga cggccacaag ttcgtgatca ccggcgaggg
     3001 catcggctac cccttcaagg gcaagcaggc catcaacctg tgcgtggtgg agggcggccc
     3061 cttgcccttc gccgaggaca tcttgtccgc cgccttcatg tacggcaacc gcgtgttcac
     3121 cgagtacccc caggacatcg tcgactactt caagaactcc tgccccgccg gctacacctg
     3181 ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc tgcaacgccg acatcaccgt
     3241 gagcgtggag gagaactgca tgtaccacga gtccaagttc tacggcgtga acttccccgc
     3301 cgacggcccc gtgatgaaga agatgaccga caactgggag ccctcctgcg agaagatcat
     3361 ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc atgtacctgc tgctgaagga
     3421 cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag gccaagtccg tgccccgcaa
     3481 gatgcccgac tggcacttca tccagcacaa gctgacccgc gaggaccgca gcgacgccaa
     3541 gaaccagaag tggcacctga ccgagcacgc catcgcctcc ggctccgcct tgccctaagg
     3601 atccggcgcg ccaggcgaag gtcacaaagg gaattaaagg cgaaggtcac aaagggaatt
     3661 aaaggcgaag gtcacaaagg gaattaaagg cgaaggtcac aaagggaatt aaaggcgaag
     3721 gtcacaaagg gaattaaagg cgaaggtcac aaagggaatt aaaggcgaag gtcacaaagg
     3781 gaatctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     3841 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     3901 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     3961 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     4021 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     4081 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     4141 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     4201 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     4261 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcctcga ggcccgggcc
     4321 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     4381 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     4441 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     4501 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     4561 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     4621 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     4681 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     4741 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     4801 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     4861 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     4921 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     4981 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaccgggt
     5041 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5101 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5161 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5221 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5281 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5341 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5401 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5461 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5521 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5581 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     5641 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     5701 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     5761 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5821 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc
     5881 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     5941 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     6001 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     6061 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     6121 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     6181 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     6241 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     6301 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6361 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6421 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6481 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6541 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat
     6601 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6661 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6721 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7141 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7201 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7261 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7321 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7381 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7441 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7501 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7561 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7621 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7681 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8761 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8881 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     8941 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     9001 ctctaaacct gtgattcctc tgaatt
//
