LOCUS       sequence-356594-        9026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses zsGreen fused to a miR-328-3p sponge and mCherry in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   McNeal AS, Belote RL, Zeng H, Urquijo M, Barker K, Torres R, Curtin 
            M, Shain AH, Andtbacka RH, Holmen S, Lum DH, McCalmont TH, 
            VanBrocklin MW, Grossman D, Wei ML, Lang UE, Judson-Torres RL
  TITLE     BRAF(V600E) induces reversible mitotic arrest in human melanocytes 
            via microrna-mediated suppression of AURKB.
  JOURNAL   Elife. 2021 Nov 23;10. pii: 70385. doi: 10.7554/eLife.70385.
  PUBMED    34812139
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-11-23"; volume: "10. pii: 70385. doi"; pages: " 
            10.7554/eLife.70385"
COMMENT     Sequence Label: pLVX-anti-MIR328-3p
FEATURES             Location/Qualifiers
     source          1..9026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    393..518
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1015..1248
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1433..1477
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1740..1855
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        1913..2216
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2217..2420
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2371..2391
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2417..2441
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2555..2564
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2561..3256
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     promoter        3458..3957
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3493..3516)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3890..3909
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3976..3985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3982..4692
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4127..4145)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4395..4414
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4706..5294
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4759..4779)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5177..5188)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5501..6134
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6263..6279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6263..6279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6276..6298)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6287..6303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6311..6317,6318..6335,6336..6341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6356..6377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6494..6511)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6665..7253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6745..6764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7424..8215,8216..8284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8047..8066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8285..8389)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8437..8571
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8474..8493)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8528..8547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(8739..9026,1..346)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
       61 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
      121 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
      181 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      241 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      301 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      361 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      421 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      481 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      541 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      601 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
      661 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
      721 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
      781 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
      841 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
      901 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
      961 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1021 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1081 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1141 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1201 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1261 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1321 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1381 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1441 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1501 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1561 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1621 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1681 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct
     1741 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1801 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     1861 tttattacag ggacagcaga gatccagttt atcgataagc ttgggagttc cgcgttacat
     1921 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     1981 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2041 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     2101 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2161 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     2221 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     2281 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     2341 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     2401 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     2461 gctgttttga cctccataga agacaccgac tctactagag gatcgctagc gctgaagctt
     2521 agatctcgag aattcgcggc cgctccggac gcgtgccacc atggcccagt ccaagcacgg
     2581 cctgaccaag gagatgacca tgaagtaccg catggagggc tgcgtggacg gccacaagtt
     2641 cgtgatcacc ggcgagggca tcggctaccc cttcaagggc aagcaggcca tcaacctgtg
     2701 cgtggtggag ggcggcccct tgcccttcgc cgaggacatc ttgtccgccg ccttcatgta
     2761 cggcaaccgc gtgttcaccg agtaccccca ggacatcgtc gactacttca agaactcctg
     2821 ccccgccggc tacacctggg accgctcctt cctgttcgag gacggcgccg tgtgcatctg
     2881 caacgccgac atcaccgtga gcgtggagga gaactgcatg taccacgagt ccaagttcta
     2941 cggcgtgaac ttccccgccg acggccccgt gatgaagaag atgaccgaca actgggagcc
     3001 ctcctgcgag aagatcatcc ccgtgcccaa gcagggcatc ttgaagggcg acgtgagcat
     3061 gtacctgctg ctgaaggacg gtggccgctt gcgctgccag ttcgacaccg tgtacaaggc
     3121 caagtccgtg ccccgcaaga tgcccgactg gcacttcatc cagcacaagc tgacccgcga
     3181 ggaccgcagc gacgccaaga accagaagtg gcacctgacc gagcacgcca tcgcctccgg
     3241 ctccgccttg ccctaaggat ccggcgcgcc acggaaggga cagagggcca gttaaacgga
     3301 agggacagag ggccagttaa acggaaggga cagagggcca gttaaacgga agggacagag
     3361 ggccagttaa acggaaggga cagagggcca gttaaacgga agggacagag ggccagttaa
     3421 acggaaggga cagagggcca gtctagataa ttctaccggg taggggaggc gcttttccca
     3481 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     3541 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     3601 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     3661 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     3721 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     3781 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     3841 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     3901 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     3961 tgcctcgagg cccgggccac catggtgagc aagggcgagg aggataacat ggccatcatc
     4021 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4081 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4141 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4201 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4261 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4321 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4381 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4441 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4501 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4561 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4621 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4681 ctgtacaagt aaccgggtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     4741 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4801 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4861 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4921 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4981 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     5041 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     5101 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     5161 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5221 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5281 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     5341 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     5401 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     5461 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
     5521 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     5581 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     5641 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     5701 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     5761 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     5821 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     5881 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     5941 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6001 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6061 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6121 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6181 gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta
     6241 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6301 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6361 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6421 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6481 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6541 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6601 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6661 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6721 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6781 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6841 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6901 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6961 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7021 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7081 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7141 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7201 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7261 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7321 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7381 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7561 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8281 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8401 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat
     8461 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8521 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     8581 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
     8641 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
     8701 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
     8761 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     8821 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     8881 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     8941 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     9001 gtttgacagc cgcctagcat ttcatc
//
