LOCUS       sequence-356599-        7824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1711-2505-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R
FEATURES             Location/Qualifiers
     source          1..7824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5130..5171
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5181..5198
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5221..5238)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5271..5405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5295..5314)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5349..5368
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6006..6594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6086..6105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6765..7556,7557..7625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7388..7407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7626..7730)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7798..7816
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttaattaagc agccaaatgt gtttgctaat gatgatagca tggtatacgg
     2761 ggaactggct agcaaaatgg aggccttcat gaacacggca atcggagtgc cgacactcca
     2821 gatccaggct agcaagatgc acatgctact caacgccctg atagcgacgc gtcgactgcg
     2881 tgaccaagag tctgccttta atctgctgac ccgcgcggtc gagggtttga ccgaaggatt
     2941 ggtcaatatg catgaaaaca tggagcaaat gaagatgtac caaaacatcc atctccgtat
     3001 ccttggcttg ctgaacaaca gtttcggtgc tccaaacaca gagcgagcgg taacaaagtg
     3061 tttcttcgat atccgagagg aggtgcgata taacgtggag gcagctcgcg ctttgattac
     3121 gtcgcacttt gtcaatctga atcagtttga cggaatgcta cgcgactgca tggacaacgg
     3181 aaacaattat gtggctatat cgttcggtat cgcactgctg gagcgcctca tcatggacga
     3241 tcgagtgatt aatattgttt cggacaacga attcatggca actgttgagc tgctaggtag
     3301 gctgacccaa caccgtcacc gctaccccga gtgtatcgtc aacgccatcg acaccctgtg
     3361 gtccggcaat ttcaatagct cctccgatta ttcccccttt aatggaaacg accgctatct
     3421 gagcggcgct agccactata ttcatagcgg aatgcaccat gtccgctcgt gtgacaccga
     3481 cgaccccccc ggactccagg aaaaaaccga attcttgttg aaagactggg tggccttgta
     3541 tacccagcaa aatcaacaat cgacccgtga cgcccgtaat tttggcgcct tcgtgcaaaa
     3601 aatgaatacc tacggcattt tgaagaccga tgatctgatt acgcgcttct ttcgtcaagc
     3661 tacccatatc tgtaccgacg tcgtctatcg tatgtttgct gaacccagcc ttcccattaa
     3721 tcaagctaag aataaaattt ttcaatggat tgacgccttt gtgcacttga tcgccatgct
     3781 ggtccgccat tcgggagagg ccggcaaccc cacgacgaaa attaatttgc tcaataaagt
     3841 cttgggaatc gtcttgggca ccttgatcaa agatcacgaa atgcgcggag tgtcctttca
     3901 acaagtggga tatcaccgtt tttttatgat gttgtttatg gaattgtgta ccgctgacgt
     3961 catcctggaa agcttgatgc atagcatcgt cagcgctttt gcttacacat atcacttgtt
     4021 gaatcccagc gtggctccgg gattctgctt tgcttggttg gagctcattt cgcatcgcgt
     4081 cttcctgggc cgcatcctgg tccaaattcc cgggcaaaag ggctggccac tttacgctca
     4141 attgctgcag gaccttttta aatatcttgc gcccttctta cgcaacactg agctcggcaa
     4201 accagtccag cttttgtaca aggggacgct acgtgttctg ctcgttctgc ttcacgactt
     4261 cccggagttc ttgtgcgact atcattttgg gttctgtgac accatcccgc ccaattgcgt
     4321 ccagatgcgt aacatcatcc tgtcggcgtt tccgcgcaac atgcgcttgc ccgatccctt
     4381 cacgcccaac ctaaaagtgg acatgctgtc ggacagtagt aatgcgccta aggtgctcag
     4441 tagctacatt atgaacatcc agcccgcgaa ctttaagaag gatttggact cgtacctcaa
     4501 ggcccgggcg ccagttactt ttctatcgga attacgtggt cacttgcagg tgacaagcga
     4561 gccaggaact cgttacaaca tggcgctgat gaatgctctg gtcatgtatg tgggaactca
     4621 ggcgattgct ttgatcagaa acaaaaactt tgtgcccaac acctcgaaca tagcacacag
     4681 cgcccacatg gatatcttcc agaatcttgc tgttgacttg gatacggagg gtcgctatct
     4741 gttcttgaat gcgattgcga accagttgcg ctatcccaac agccacacgc actacttcag
     4801 ctgtgcggtg ctgcatctgt ttgccgaggc caactcggag gcaattcagg agcagatcac
     4861 tcgcgtactt ctggaacgat tgatcgtgaa ccgcccgcat ccttggggac tgttgatcac
     4921 ttttattgag ctgatcaaaa atcccatcta caagttctgg gaccatgact ttgtgcactg
     4981 tgcgccagag atcaccaagc tctttgaatc ggtggcccgt tcctgtctgg ccaagtcgaa
     5041 cgtcacccag cagttgaaca tgcccgtcgt cgatggcgag ggccaggagg tagccaccat
     5101 caactgatct agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc
     5161 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
     5221 cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca
     5281 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5341 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5401 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     5461 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg
     5521 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     5581 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     5641 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     5701 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
     5761 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
     5821 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
     5881 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
     5941 catcagggac agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6181 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6421 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6541 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6601 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6661 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6721 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6781 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6841 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6901 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6961 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7021 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7081 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7141 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7201 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7261 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7321 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7381 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7441 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7501 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7561 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7621 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7681 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7741 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7801 aggcgtatca cgaggccctt tcgt
//
