LOCUS       sequence-356606-       13207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by 
            zebrafish gfap promoter for Tol2 mediated recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Lee SH, Nam TS, Kim KH, Kim JH, Yoon W, Heo SH, Kim MJ, Shin BA, 
            Perng MD, Choy HE, Jo J, Kim MK, Choi SY
  TITLE     Aggregation-prone GFAP mutation in Alexander disease validated using
            a zebrafish model.
  JOURNAL   BMC Neurol. 2017 Sep 7;17(1):175. doi: 10.1186/s12883-017-0938-7.
  PUBMED    28882119
REFERENCE   2  (bases 1 to 13207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Neurol."; date: "2017-09-7"; volume: "17(1):175. doi"; pages: " 
            10.1186/s12883-017-0938-7"
COMMENT     Sequence Label: pSYC-204
FEATURES             Location/Qualifiers
     source          1..13207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(679..690,691..828)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             9586..9609
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9613..9636
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9640..9663
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(9712..9714,9715..9717,9718..10431)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9712..9714,9715..9717,9718..10428)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9757..9778)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10018..10037)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10365..10386
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    10554..10675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10591..10610)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10645..10664
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10682..10698)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(10737..10924,10925..10936)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10970..10986)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11023..11043)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11023..11041)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11062..11078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11062..11078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11075..11097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11086..11102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11110..11116,11117..11134,11135..11140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11155..11176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11293..11310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11464..12052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11544..11563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12223..13014,13015..13083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12846..12865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13084..13188)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc nggsgggtgt ggkggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattag atctcgaggt
      841 aaggactgag gtgatgcaca gtgttgggta tgttactttc aataagttat tctttgctaa
      901 ttattagtca tttgaattgt attgaaatta catttttagt tactttgcct aaaaaaaaac
      961 ttgataagta atcgaattac atacttgaag tccacataaa tctcattcat tcattccttc
     1021 tcttttcgac ttagtccctt tattaatcag gggtttctac agcggaacga acctcatatg
     1081 tttcaattta gtgactattt tttataaatt attgacaatg tttttgttgc ttgttcaaac
     1141 tacttattta aaatgagctg aaactgcaca attattgata tttcattgga acagcttaat
     1201 tttttttatg tttaatccac ttaaataaaa ttccaattat ataatattcc aattataaag
     1261 ttacttaatc aatttgtatt aggacaatat gaatgaattg tgtggaaccc tgcatttttt
     1321 acagtgaacc tttccttgtt tcttaaacat gggtgatttt cggagaaata aaatttgaca
     1381 atatctgaaa tgtaaaaaga aaatattcag ttactctgcc tttctaaatg catagtttat
     1441 attatcatca gagttgtaaa gtaactagtt acaaatactc aaattactgt aattgagtag
     1501 tttttttcag gaattgtaat ttactaagta gctaaaaaat gtgtactttt actttccctt
     1561 gagtacatta ttaatgctgt aacggtactt ttactccact actttccttc agcctgcact
     1621 cactactttg ttttatcttg tctatgagga ttagaaaaat caatcctgtg attccaatca
     1681 aatcgcacat ccaaagtctt tacaaaagca ttttgtttgg atgtgaaaat cgggttgatg
     1741 tcagaactca atgtcagccc aacgtcaatg tccaacctaa aatcaaccta atatcaacgt
     1801 ctaatgatgt tacagcttga cgttgtgtgg gcgttaccat tatttgtcag acattggatt
     1861 ttagttgcca tacctgataa ataaatgtca gtatttgatg tccgtatgat cttggattta
     1921 gatgttggct cgacattgga ttttggtcac ctgacataac aacctaaatc tatcctaata
     1981 ataacgtctt atgacgttgt gtgccaacca tttttgttta ttaaaaattt atataaacat
     2041 ttacaataat atattatcca tgtgatttaa tagaaatgtt atttgttttt tttctccccc
     2101 atatttctga atgacttaaa gcttttatta tcaggataag gttgtggttt ttattttttg
     2161 cttgctaact tcaattgcta ttgcatttac atttatgcaa atggggagac gcggtgggtc
     2221 agttggcgtt tctgtgtgga gtttgcatgt tctccccgtg ttcgcgtggg ttttctctgg
     2281 gtgctctggt ttcccctaaa gacatgtagt ataggtgaat tgggtaagct aaattgtccg
     2341 tagtgtgtat gtgtgaatgt atggatgttt tcctgtgatg ggttgcagct gaaagggcat
     2401 ccgctgtgta aaacatatgc tggataagtt ggcaattcat ttcgctgtgg taaccccaga
     2461 ttaatatcgg aactacgcca aaaagaaaat gaatgaatga gtgaattaat gcaaatggct
     2521 catccttcca cctaattcca gtgccttgca ttgcattcaa gctctacaat taatcagttc
     2581 ctttattttc actttccaaa ctgaatccaa caactcatga ggaccccagg gtgccttgat
     2641 ttaccagtgt catgatgaac acaatctacc ccactttaac ccactcgtca acataaatac
     2701 tcccttaaag ggtgtacttg tgtctgagta gaagtgtctg ctgggttgga ggtgtcgcac
     2761 attccaggac acgtcaagaa gaacgctaag agcaacagga gaacaatgtc acaaaaaagg
     2821 tcaaagaaac gccatccatt agccagcttt ataaacagct gtctcgtggc taaagaagtt
     2881 ctcaagaata accaaccttg catttttcca cattagtttt acaccgcagc aaagtcacca
     2941 tgtggattta tagcatccaa accccatttt caatgagagg cttcacattt ctcgctctca
     3001 ttcaatccca cactgcctct cttttcagac taaatcctga taggatgagt ggtatggggg
     3061 cggggagggg gcacaatacc tcagtccagc ttccacaaag actcaacaca cccatgcaga
     3121 tgtcctgttg ttggggaagt gcatttgtaa ccaaatttca aactttcaac tttcatttga
     3181 agcagtggag actgctccat tcctattcaa gatcaattta ttgaaatatg acaacattta
     3241 gaataacttt gttatatgtt attatgtttt ataatgcaat tttgtttctg tgacatacat
     3301 ttttttattt agtgcagtct tcagagccac aggaaccttg agaatcattc taattatgat
     3361 ttgctactcg aaaaacattt ttcatcatca ataataagaa attaaaacgg tatacatata
     3421 gatgtttagt ataacagttc ttagtagttt aaaagaacat ttgtaacaac attccaacct
     3481 tttaataact ttagccttgc ttacatttct aaaggagagc cttccagctg tttttctgaa
     3541 cccagtgatg aatgtaaagt tcttaaaacc aagttctaag aatgttttaa tgattacatt
     3601 taacccaatt tcaagccaaa tttcctatac cacgagtaaa atatagttat aattaggata
     3661 gatctttggt aaaataagat acaattttaa gatttgaatt taaaagtagc taaattgttt
     3721 ttttatgaca gaacttttaa gtttatagaa tattaataca ggacaaaact tgtaaatgta
     3781 tttaatttcc tatgaatatt tgataaaata taaatgacat ctatatgtaa attcatgaac
     3841 catgactctt atgaattatc agtgcacaaa ttatgtttga tgcacactta aggtcaaaag
     3901 tttagggtca gtttattttt tttttatttt taattttatt taataattta aataaaatta
     3961 ttctgttcat caaggcacat ttattaaatg taacaataaa ttgttaaata cttattaaat
     4021 atttattttt tacttattgt atgcagtttc aattgaagtc attattgctt ttattactat
     4081 taccattaat aaaaataaca ttaataataa taataataat aataataatt gatattacag
     4141 tgatttctga aggatcacgt gactgaagac tggagtaatg aagctgaaaa ctcatcattt
     4201 aaatcactgg agtaaatgat tcaattaaat gatcatttaa tgatcattct tttgaacagt
     4261 tattttatag tgcaataaca tttcacagtt ttaccatttg ttttgtattt ttgattcaat
     4321 aaatgcagcc ttggtgagca ggagaagctt aatttaaaac atttaaaact cctactgacc
     4381 ccaaactttt gaccggtagt gtacatgtat tactgtatgt tcaaactata tgcttttatt
     4441 cataagtgtt tctattgctg tacatttgac tgtctgcatc aggtgaagtg tatgaaagag
     4501 tgtgtgttta ctccaccccg tgggaaagag cagtagctgc aatgtgtgtg tgtgagtagt
     4561 tagtgatgca tgagtgatcc actgaaccct gtccagcccc ctgctgcagc tctcaacaca
     4621 tcatgatagg tcacctctac tgggaggctt tcagcacagc tctcacaact gatttatcag
     4681 agagtactct gaaatggaga aattactttc atattaaaga gcacacacac acacacacac
     4741 acacacacac acacacacac acacacacac acacacacac acacacctag ttcaccttat
     4801 taacgtggtt aagcctttaa atgtcacttt aagctgtata gaagtgtctt gaaaaatatc
     4861 tattcaaata ttatttactg tcatcaagga aaagataaaa taaatcagtt attagaaata
     4921 agtttttaaa actattatac ttagaaatgt gctgaaacaa tcttcctaga acagaacaga
     4981 aattggggaa aaaataaaca aaaataaata aatctatcta tctatagtgt gtataatgtg
     5041 tgtagtgagt atgtttaaaa taggtcaact aatatgttca taataaaaat acagcatcaa
     5101 tggtttgggt gtttttagat ccaaaggtct taaacgtggt tattgctggt tgttcagttt
     5161 tttctaggct ctaagaaaaa tcattttaga aactgtaact gtttccttat ccatctgaat
     5221 tccacctttg ggatgtagtg gaacgggaga tcaactgcat gatgctatca agtcaatatg
     5281 gatcaaaatc tcaggaatat ttccaggacc ttattgaatc tatgccatga aggattaagg
     5341 cagttctgaa aggggaaatg cggtttaacc cggtaatagc aaggtgtacc taataaagtg
     5401 gccggtgagt gtatatattg ttattgtttc accttcagaa atgacatatc tagacaatag
     5461 tttccttttt cttttttaat tattgtactt ctgtaagatt cttcaatgtt caatggtttt
     5521 cttatgttaa aggcgcagag ctgtagtttt agtgaagtaa cagcattccg ttgaccaaag
     5581 ctgacagatg aaggagagtc agttctgacc atgcagttgt cagtcacaat taagcctggt
     5641 ttggttacaa gttaaacgta gacctgcttg tggtcaggat catcctttaa aagaactaga
     5701 tttgttttta acaatttgta accggtgtgc tctgaagtgt cttaaataaa gcatatagga
     5761 agcaaagcag tgaaaaatgt tccttataat ctacgagcat tagcttaaat gtataaacaa
     5821 aataatatta attgtgtgtt cagatcaaat ataggtattg ttacacctaa aataaaccca
     5881 ttgctttaaa aaataattaa acacagtgta caatattagc atacttcctc tgacatctct
     5941 aagtaaaaca agagctttca cgatttactc actgacccat cttacagcac ttctctgtga
     6001 atttaggcca tcacaaatgc tgtgcccagt tctctgactg ggtttgacag tcgcccatct
     6061 gtgtccagca gtctgatgag ctcatcctct gtaaatctgg ctggctgccg tgttgttttg
     6121 tgtctgtccg tgagtctctc tggccttcat acactcctct gctacttcat agactgctga
     6181 tgagtgcaga ggccaaacca ttagtgcttt ccttttttta gacctcagta atgcacaaca
     6241 atgcaaaaca agtagattga gccttttgtt ctttggactg agcatttggt tagaatagct
     6301 tgaagggata gttagaagat attttgaagg gaaactaaaa acctgttacc atttgacatc
     6361 caaagaagga aaactaatat gattgagtgg ttacagattt ccaacacttt tcaacatata
     6421 ttattttgtg ttccactaaa gaaacagtca gatattatac tgtcaaccaa agtactgcac
     6481 ttgactcaaa aaagctctgc agcaagttgc ctcttattct aggtcgagtc aacctttttt
     6541 tctctctctc tgtcggttca ttcaaggttc agtaaatgat gacagaattt tcagctttta
     6601 ttttgggtga actaaccctt tatatatttg ttgtaagttt atttttctgt atattataaa
     6661 tatattagca acataattct aagctacact tgtacccaat caagggtcac actttatttt
     6721 gatggtatgt ttgtttgaat ttaagttaca ttgcatctac atgtcaacta attctcatta
     6781 gattataaga agactgttag gttggggcta gtgtaagttg acatgtactt gcaaagtttc
     6841 tcagagtcag ttaaatgtct gttttgtagc agtattaaca gatattaagc acactgtata
     6901 ttaatactca aatgaaccgt caaaataaag tgttaccgta aaaattattt ccacatctca
     6961 aggtagcttt atgaggaaag ttttctttaa gattagtttc ttcgaacaaa cttgcatgtt
     7021 aaatatgtgc tcagatatgg ggcacacaga caaaatgttt attggacgaa taaccccttt
     7081 aacagttaac cagcattttt taaagggata gtcagaagat attttgaagg aaactaaaaa
     7141 cctgtaacca ttcgacaatc gaagaaggta aaagaaatat gatagagtag ttacagtttt
     7201 tcaacactct ttataatata ttgtttagta ttcaactgaa gaaatagtca aacacactgt
     7261 aaaaaaagtt ggtttaaatt gactcaactt tcaactcaag tatagtactt gacttgattt
     7321 aagttgagtt aactagacta aactaaaagc actaaaagca ctgcggcaag tcgcctttca
     7381 attttaggtt gagtaaatta ttattatttt tttttacagt tcaggttcag taaatgataa
     7441 cataattttc agttttgggt gaactatccc tttatatatt tgttgtaagt taatttttct
     7501 gtaaattata aattaattag caaaaaagct gcacttcttc accattaatt aattaataca
     7561 tctcaaagta gctttataag gaaagttttc tttaattatg tttctttgca tgttaaaatt
     7621 gtgctcagat atggagcaca caagcaaaat gtttattgga tgaataaccc ctttaacatt
     7681 taaccagcat gagttcaagt aaacaaatca attcctatga tttttctgct aaaccgatat
     7741 aaatcagtgc tgctgaagag catgtgtgtg atgttctcag atcagcacaa aagagggagc
     7801 gccagtttct tttaataatg aagaattacg taatcacctc ctgtgttccc tgaaaccatt
     7861 aaactcccag acagaacaaa actgtgactg agaaagcaga gcaactccgg agtaactcag
     7921 aagatctgca aaccaactct ttagcatgag gtaagagaga attaaagtcc aatatcagtg
     7981 ctgatgccat ctcagtctct ctatttctgt gttccagcag ggtatgaata ggattttctc
     8041 tggctcactg tgaaaagagg cagtcaggag actggctgga caagcacaaa caattgcggg
     8101 ctcacaaaca ccccctcggc ctttgccctc cccatcagac cccccctctc tctttcaccc
     8161 cccacatcaa ctctataaaa acccagagtt cacccgcact cgatctcatt ctcctccacc
     8221 atggagtccc agcgttcctc gtcgatcgac ggatccatgg agaggagacg catcacctcc
     8281 gctgctcgcc gctcctacgt ctcctcaggg gagatgatgg tggggggcct ggctcctggc
     8341 cgccgtctgg gtcctggcac ccgcctctcc ctggctcgaa tgccccctcc actcccgacc
     8401 cgggtggatt tctccctggc tggggcactc aatgctggct tcaaggagac ccgggccagt
     8461 gagcgggcag agatgatgga gctcaatgac tgctttgcca gctacatcga gaaggttcgc
     8521 ttcctggaac agcaaaacaa ggcgctggct gctgagctga accagctgcg ggccaaggag
     8581 cccaccaagc tggcagacgt ctaccaggct gagctgcgag agctgcggct gcggctcgat
     8641 caactcaccg ccaacagcgc ccggctggag gttgagaggg acaatctggc acaggacctg
     8701 gccactgtga ggcagaagct ccaggatgaa accaacctga ggctggaagc cgagaacaac
     8761 ctggctgcct atagacagga agcagatgaa gccaccctgg cccgtctgga tctggagagg
     8821 aagattgagt cgctggagga ggagatccgg ttcttgagga agatccacga ggaggaggtt
     8881 cgggaactcc aggagcagct ggcccgacag caggtccatg tggagcttga cgtggccaag
     8941 ccagacctca ccgcagccct gaaagagatc cgcacgcagt atgaggcaat ggcgtccagc
     9001 aacatgcatg aagccgaaga gtggtaccgc tccaagtttg cagacctgac agacgctgct
     9061 gcccgcaacg cggagctgct ccgccaggcc aagcacgaag ccaacgacta ccggcgccag
     9121 ttgcagtcct tgacctgcga cctggagtct ctgcgcggca cgaacgagtc cctggagagg
     9181 cagatgcgcg agcaggagga gcggcacgtg cgggaggcgg ccagttatca ggaggcgctg
     9241 gcgcggctgg aggaagaggg gcagagcctc aaggacgaga tggcccgcca cttgcaggag
     9301 taccaggacc tgctcaatgt caagctggcc ctggacatcg agatcgccac ctacaggaag
     9361 ctgctagagg gcgaggagaa ccggatcacc attcccgtgc agaccttctc caacctgcag
     9421 attcgagaaa ccagcctgga caccaagtct gtgtcagaag gccacctcaa gaggaacatc
     9481 gtggtgaaga ccgtggagat gcgggatgga gaggtcatta aggagtccaa gcaggagcac
     9541 aaggatgtga tgatgatatc tggcgcgcct accatggggc ccggcgacta caaggatgac
     9601 gatgacaagg gcgactacaa ggatgacgat gacaagggcg actacaagga tgacgatgac
     9661 aagggccggc ctaagatcac cggtcccggg cttaaggagc tcgcatgcac catggtgagc
     9721 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     9781 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     9841 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     9901 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     9961 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
    10021 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
    10081 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
    10141 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
    10201 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
    10261 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
    10321 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
    10381 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc
    10441 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    10501 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    10561 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10621 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
    10681 agatccacta gttctagagc ggccgccacc gcggtggagc tcctcgagcc gggcccaagt
    10741 gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact
    10801 tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca
    10861 agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa
    10921 gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag atccactagt
    10981 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
    11041 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
    11101 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    11161 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    11221 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    11281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    11341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    11401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    11461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    11521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    11581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    11641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    11701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    11761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    11821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    11941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    12001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    12061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    12121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    12181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    12241 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    12301 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    12361 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    12421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    12481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    12541 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    12601 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    12661 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    12721 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    12781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    12841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    12901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    12961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    13021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    13081 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    13141 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    13201 aaaagtg
//
