LOCUS       sequence-356608-       13206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap 
            promoter for Tol2 mediated recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Lee SH, Nam TS, Kim KH, Kim JH, Yoon W, Heo SH, Kim MJ, Shin BA, 
            Perng MD, Choy HE, Jo J, Kim MK, Choi SY
  TITLE     Aggregation-prone GFAP mutation in Alexander disease validated using
            a zebrafish model.
  JOURNAL   BMC Neurol. 2017 Sep 7;17(1):175. doi: 10.1186/s12883-017-0938-7.
  PUBMED    28882119
REFERENCE   2  (bases 1 to 13206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Neurol."; date: "2017-09-7"; volume: "17(1):175. doi"; pages: " 
            10.1186/s12883-017-0938-7"
COMMENT     Sequence Label: pSYC-201
FEATURES             Location/Qualifiers
     source          1..13206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(678..689,690..827)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             9585..9608
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9612..9635
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9639..9662
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(9711..9713,9714..9716,9717..10430)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9711..9713,9714..9716,9717..10427)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9756..9777)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10017..10036)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10364..10385
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    10553..10674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10590..10609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10644..10663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10681..10697)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(10736..10923,10924..10935)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10969..10985)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11022..11042)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11022..11040)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11061..11077)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11061..11077)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11074..11096)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11085..11101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11109..11115,11116..11133,11134..11139))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11154..11175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11292..11309)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11463..12051)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11543..11562)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12222..13013,13014..13082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12845..12864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13083..13187)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc ggcgggtgtg gtggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
      661 cggcatatgg ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      721 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      781 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaga tctcgaggta
      841 aggactgagg tgatgcacag tgttgggtat gttactttca ataagttatt ctttgctaat
      901 tattagtcat ttgaattgta ttgaaattac atttttagtt actttgccta aaaaaaaact
      961 tgataagtaa tcgaattaca tacttgaagt ccacataaat ctcattcatt cattccttct
     1021 cttttcgact tagtcccttt attaatcagg ggtttctaca gcggaacgaa cctcatatgt
     1081 ttcaatttag tgactatttt ttataaatta ttgacaatgt ttttgttgct tgttcaaact
     1141 acttatttaa aatgagctga aactgcacaa ttattgatat ttcattggaa cagcttaatt
     1201 ttttttatgt ttaatccact taaataaaat tccaattata taatattcca attataaagt
     1261 tacttaatca atttgtatta ggacaatatg aatgaattgt gtggaaccct gcatttttta
     1321 cagtgaacct ttccttgttt cttaaacatg ggtgattttc ggagaaataa aatttgacaa
     1381 tatctgaaat gtaaaaagaa aatattcagt tactctgcct ttctaaatgc atagtttata
     1441 ttatcatcag agttgtaaag taactagtta caaatactca aattactgta attgagtagt
     1501 ttttttcagg aattgtaatt tactaagtag ctaaaaaatg tgtactttta ctttcccttg
     1561 agtacattat taatgctgta acggtacttt tactccacta ctttccttca gcctgcactc
     1621 actactttgt tttatcttgt ctatgaggat tagaaaaatc aatcctgtga ttccaatcaa
     1681 atcgcacatc caaagtcttt acaaaagcat tttgtttgga tgtgaaaatc gggttgatgt
     1741 cagaactcaa tgtcagccca acgtcaatgt ccaacctaaa atcaacctaa tatcaacgtc
     1801 taatgatgtt acagcttgac gttgtgtggg cgttaccatt atttgtcaga cattggattt
     1861 tagttgccat acctgataaa taaatgtcag tatttgatgt ccgtatgatc ttggatttag
     1921 atgttggctc gacattggat tttggtcacc tgacataaca acctaaatct atcctaataa
     1981 taacgtctta tgacgttgtg tgccaaccat ttttgtttat taaaaattta tataaacatt
     2041 tacaataata tattatccat gtgatttaat agaaatgtta tttgtttttt ttctccccca
     2101 tatttctgaa tgacttaaag cttttattat caggataagg ttgtggtttt tattttttgc
     2161 ttgctaactt caattgctat tgcatttaca tttatgcaaa tggggagacg cggtgggtca
     2221 gttggcgttt ctgtgtggag tttgcatgtt ctccccgtgt tcgcgtgggt tttctctggg
     2281 tgctctggtt tcccctaaag acatgtagta taggtgaatt gggtaagcta aattgtccgt
     2341 agtgtgtatg tgtgaatgta tggatgtttt cctgtgatgg gttgcagctg aaagggcatc
     2401 cgctgtgtaa aacatatgct ggataagttg gcaattcatt tcgctgtggt aaccccagat
     2461 taatatcgga actacgccaa aaagaaaatg aatgaatgag tgaattaatg caaatggctc
     2521 atccttccac ctaattccag tgccttgcat tgcattcaag ctctacaatt aatcagttcc
     2581 tttattttca ctttccaaac tgaatccaac aactcatgag gaccccaggg tgccttgatt
     2641 taccagtgtc atgatgaaca caatctaccc cactttaacc cactcgtcaa cataaatact
     2701 cccttaaagg gtgtacttgt gtctgagtag aagtgtctgc tgggttggag gtgtcgcaca
     2761 ttccaggaca cgtcaagaag aacgctaaga gcaacaggag aacaatgtca caaaaaaggt
     2821 caaagaaacg ccatccatta gccagcttta taaacagctg tctcgtggct aaagaagttc
     2881 tcaagaataa ccaaccttgc atttttccac attagtttta caccgcagca aagtcaccat
     2941 gtggatttat agcatccaaa ccccattttc aatgagaggc ttcacatttc tcgctctcat
     3001 tcaatcccac actgcctctc ttttcagact aaatcctgat aggatgagtg gtatgggggc
     3061 ggggaggggg cacaatacct cagtccagct tccacaaaga ctcaacacac ccatgcagat
     3121 gtcctgttgt tggggaagtg catttgtaac caaatttcaa actttcaact ttcatttgaa
     3181 gcagtggaga ctgctccatt cctattcaag atcaatttat tgaaatatga caacatttag
     3241 aataactttg ttatatgtta ttatgtttta taatgcaatt ttgtttctgt gacatacatt
     3301 tttttattta gtgcagtctt cagagccaca ggaaccttga gaatcattct aattatgatt
     3361 tgctactcga aaaacatttt tcatcatcaa taataagaaa ttaaaacggt atacatatag
     3421 atgtttagta taacagttct tagtagttta aaagaacatt tgtaacaaca ttccaacctt
     3481 ttaataactt tagccttgct tacatttcta aaggagagcc ttccagctgt ttttctgaac
     3541 ccagtgatga atgtaaagtt cttaaaacca agttctaaga atgttttaat gattacattt
     3601 aacccaattt caagccaaat ttcctatacc acgagtaaaa tatagttata attaggatag
     3661 atctttggta aaataagata caattttaag atttgaattt aaaagtagct aaattgtttt
     3721 tttatgacag aacttttaag tttatagaat attaatacag gacaaaactt gtaaatgtat
     3781 ttaatttcct atgaatattt gataaaatat aaatgacatc tatatgtaaa ttcatgaacc
     3841 atgactctta tgaattatca gtgcacaaat tatgtttgat gcacacttaa ggtcaaaagt
     3901 ttagggtcag tttatttttt ttttattttt aattttattt aataatttaa ataaaattat
     3961 tctgttcatc aaggcacatt tattaaatgt aacaataaat tgttaaatac ttattaaata
     4021 tttatttttt acttattgta tgcagtttca attgaagtca ttattgcttt tattactatt
     4081 accattaata aaaataacat taataataat aataataata ataataattg atattacagt
     4141 gatttctgaa ggatcacgtg actgaagact ggagtaatga agctgaaaac tcatcattta
     4201 aatcactgga gtaaatgatt caattaaatg atcatttaat gatcattctt ttgaacagtt
     4261 attttatagt gcaataacat ttcacagttt taccatttgt tttgtatttt tgattcaata
     4321 aatgcagcct tggtgagcag gagaagctta atttaaaaca tttaaaactc ctactgaccc
     4381 caaacttttg accggtagtg tacatgtatt actgtatgtt caaactatat gcttttattc
     4441 ataagtgttt ctattgctgt acatttgact gtctgcatca ggtgaagtgt atgaaagagt
     4501 gtgtgtttac tccaccccgt gggaaagagc agtagctgca atgtgtgtgt gtgagtagtt
     4561 agtgatgcat gagtgatcca ctgaaccctg tccagccccc tgctgcagct ctcaacacat
     4621 catgataggt cacctctact gggaggcttt cagcacagct ctcacaactg atttatcaga
     4681 gagtactctg aaatggagaa attactttca tattaaagag cacacacaca cacacacaca
     4741 cacacacaca cacacacaca cacacacaca cacacacaca cacacctagt tcaccttatt
     4801 aacgtggtta agcctttaaa tgtcacttta agctgtatag aagtgtcttg aaaaatatct
     4861 attcaaatat tatttactgt catcaaggaa aagataaaat aaatcagtta ttagaaataa
     4921 gtttttaaaa ctattatact tagaaatgtg ctgaaacaat cttcctagaa cagaacagaa
     4981 attggggaaa aaataaacaa aaataaataa atctatctat ctatagtgtg tataatgtgt
     5041 gtagtgagta tgtttaaaat aggtcaacta atatgttcat aataaaaata cagcatcaat
     5101 ggtttgggtg tttttagatc caaaggtctt aaacgtggtt attgctggtt gttcagtttt
     5161 ttctaggctc taagaaaaat cattttagaa actgtaactg tttccttatc catctgaatt
     5221 ccacctttgg gatgtagtgg aacgggagat caactgcatg atgctatcaa gtcaatatgg
     5281 atcaaaatct caggaatatt tccaggacct tattgaatct atgccatgaa ggattaaggc
     5341 agttctgaaa ggggaaatgc ggtttaaccc ggtaatagca aggtgtacct aataaagtgg
     5401 ccggtgagtg tatatattgt tattgtttca ccttcagaaa tgacatatct agacaatagt
     5461 ttcctttttc ttttttaatt attgtacttc tgtaagattc ttcaatgttc aatggttttc
     5521 ttatgttaaa ggcgcagagc tgtagtttta gtgaagtaac agcattccgt tgaccaaagc
     5581 tgacagatga aggagagtca gttctgacca tgcagttgtc agtcacaatt aagcctggtt
     5641 tggttacaag ttaaacgtag acctgcttgt ggtcaggatc atcctttaaa agaactagat
     5701 ttgtttttaa caatttgtaa ccggtgtgct ctgaagtgtc ttaaataaag catataggaa
     5761 gcaaagcagt gaaaaatgtt ccttataatc tacgagcatt agcttaaatg tataaacaaa
     5821 ataatattaa ttgtgtgttc agatcaaata taggtattgt tacacctaaa ataaacccat
     5881 tgctttaaaa aataattaaa cacagtgtac aatattagca tacttcctct gacatctcta
     5941 agtaaaacaa gagctttcac gatttactca ctgacccatc ttacagcact tctctgtgaa
     6001 tttaggccat cacaaatgct gtgcccagtt ctctgactgg gtttgacagt cgcccatctg
     6061 tgtccagcag tctgatgagc tcatcctctg taaatctggc tggctgccgt gttgttttgt
     6121 gtctgtccgt gagtctctct ggccttcata cactcctctg ctacttcata gactgctgat
     6181 gagtgcagag gccaaaccat tagtgctttc ctttttttag acctcagtaa tgcacaacaa
     6241 tgcaaaacaa gtagattgag ccttttgttc tttggactga gcatttggtt agaatagctt
     6301 gaagggatag ttagaagata ttttgaaggg aaactaaaaa cctgttacca tttgacatcc
     6361 aaagaaggaa aactaatatg attgagtggt tacagatttc caacactttt caacatatat
     6421 tattttgtgt tccactaaag aaacagtcag atattatact gtcaaccaaa gtactgcact
     6481 tgactcaaaa aagctctgca gcaagttgcc tcttattcta ggtcgagtca accttttttt
     6541 ctctctctct gtcggttcat tcaaggttca gtaaatgatg acagaatttt cagcttttat
     6601 tttgggtgaa ctaacccttt atatatttgt tgtaagttta tttttctgta tattataaat
     6661 atattagcaa cataattcta agctacactt gtacccaatc aagggtcaca ctttattttg
     6721 atggtatgtt tgtttgaatt taagttacat tgcatctaca tgtcaactaa ttctcattag
     6781 attataagaa gactgttagg ttggggctag tgtaagttga catgtacttg caaagtttct
     6841 cagagtcagt taaatgtctg ttttgtagca gtattaacag atattaagca cactgtatat
     6901 taatactcaa atgaaccgtc aaaataaagt gttaccgtaa aaattatttc cacatctcaa
     6961 ggtagcttta tgaggaaagt tttctttaag attagtttct tcgaacaaac ttgcatgtta
     7021 aatatgtgct cagatatggg gcacacagac aaaatgttta ttggacgaat aaccccttta
     7081 acagttaacc agcatttttt aaagggatag tcagaagata ttttgaagga aactaaaaac
     7141 ctgtaaccat tcgacaatcg aagaaggtaa aagaaatatg atagagtagt tacagttttt
     7201 caacactctt tataatatat tgtttagtat tcaactgaag aaatagtcaa acacactgta
     7261 aaaaaagttg gtttaaattg actcaacttt caactcaagt atagtacttg acttgattta
     7321 agttgagtta actagactaa actaaaagca ctaaaagcac tgcggcaagt cgcctttcaa
     7381 ttttaggttg agtaaattat tattattttt ttttacagtt caggttcagt aaatgataac
     7441 ataattttca gttttgggtg aactatccct ttatatattt gttgtaagtt aatttttctg
     7501 taaattataa attaattagc aaaaaagctg cacttcttca ccattaatta attaatacat
     7561 ctcaaagtag ctttataagg aaagttttct ttaattatgt ttctttgcat gttaaaattg
     7621 tgctcagata tggagcacac aagcaaaatg tttattggat gaataacccc tttaacattt
     7681 aaccagcatg agttcaagta aacaaatcaa ttcctatgat ttttctgcta aaccgatata
     7741 aatcagtgct gctgaagagc atgtgtgtga tgttctcaga tcagcacaaa agagggagcg
     7801 ccagtttctt ttaataatga agaattacgt aatcacctcc tgtgttccct gaaaccatta
     7861 aactcccaga cagaacaaaa ctgtgactga gaaagcagag caactccgga gtaactcaga
     7921 agatctgcaa accaactctt tagcatgagg taagagagaa ttaaagtcca atatcagtgc
     7981 tgatgccatc tcagtctctc tatttctgtg ttccagcagg gtatgaatag gattttctct
     8041 ggctcactgt gaaaagaggc agtcaggaga ctggctggac aagcacaaac aattgcgggc
     8101 tcacaaacac cccctcggcc tttgccctcc ccatcagacc ccccctctct ctttcacccc
     8161 ccacatcaac tctataaaaa cccagagttc acccgcactc gatctcattc tcctccacca
     8221 tggagtccca gcgttcctcg tcgacggatc gatccatgga gaggagacgc atcacctccg
     8281 ctgctcgccg ctcctacgtc tcctcagggg agatgatggt ggggggcctg gctcctggcc
     8341 gccgtctggg tcctggcacc cgcctctccc tggctcgaat gccccctcca ctcccgaccc
     8401 gggtggattt ctccctggct ggggcactca atgctggctt caaggagacc cgggccagtg
     8461 agcgggcaga gatgatggag ctcaatgacc gctttgccag ctacatcgag aaggttcgct
     8521 tcctggaaca gcaaaacaag gcgctggctg ctgagctgaa ccagctgcgg gccaaggagc
     8581 ccaccaagct ggcagacgtc taccaggctg agctgcgaga gctgcggctg cggctcgatc
     8641 aactcaccgc caacagcgcc cggctggagg ttgagaggga caatctggca caggacctgg
     8701 ccactgtgag gcagaagctc caggatgaaa ccaacctgag gctggaagcc gagaacaacc
     8761 tggctgccta tagacaggaa gcagatgaag ccaccctggc ccgtctggat ctggagagga
     8821 agattgagtc gctggaggag gagatccggt tcttgaggaa gatccacgag gaggaggttc
     8881 gggaactcca ggagcagctg gcccgacagc aggtccatgt ggagcttgac gtggccaagc
     8941 cagacctcac cgcagccctg aaagagatcc gcacgcagta tgaggcaatg gcgtccagca
     9001 acatgcatga agccgaagag tggtaccgct ccaagtttgc agacctgaca gacgctgctg
     9061 cccgcaacgc ggagctgctc cgccaggcca agcacgaagc caacgactac cggcgccagt
     9121 tgcagtcctt gacctgcgac ctggagtctc tgcgcggcac gaacgagtcc ctggagaggc
     9181 agatgcgcga gcaggaggag cggcacgtgc gggaggcggc cagttatcag gaggcgctgg
     9241 cgcggctgga ggaagagggg cagagcctca aggacgagat ggcccgccac ttgcaggagt
     9301 accaggacct gctcaatgtc aagctggccc tggacatcga gatcgccacc tacaggaagc
     9361 tgctagaggg cgaggagaac cggatcacca ttcccgtgca gaccttctcc aacctgcaga
     9421 ttcgagaaac cagcctggac accaagtctg tgtcagaagg ccacctcaag aggaacatcg
     9481 tggtgaagac cgtggagatg cgggatggag aggtcattaa ggagtccaag caggagcaca
     9541 aggatgtgat gatgatatct ggcgcgccta ccatggggcc cggcgactac aaggatgacg
     9601 atgacaaggg cgactacaag gatgacgatg acaagggcga ctacaaggat gacgatgaca
     9661 agggccggcc taagatcacc ggtcccgggc ttaaggagct cgcatgcacc atggtgagca
     9721 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     9781 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     9841 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     9901 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     9961 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
    10021 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
    10081 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
    10141 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
    10201 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
    10261 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
    10321 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
    10381 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
    10441 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    10501 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    10561 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10621 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcga
    10681 gatccactag ttctagagcg gccgccaccg cggtggagct cctcgagccg ggcccaagtg
    10741 atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt
    10801 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
    10861 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
    10921 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
    10981 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
    11041 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    11101 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    11161 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    11221 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    11281 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    11341 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    11401 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    11461 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    11521 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    11581 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    11641 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11701 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11761 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11821 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11881 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    11941 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    12001 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    12061 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    12121 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    12181 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    12241 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    12301 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    12361 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    12421 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    12481 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    12541 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    12601 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    12661 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    12721 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    12781 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    12841 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    12901 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    12961 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    13021 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    13081 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    13141 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    13201 aaagtg
//
