LOCUS       sequence-356614-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Williams LM, Sridhar S, Samaroo J, Peart J, Adindu EK, Addanki A, 
            DiRusso CJ, Aguirre Carrion PJ, Rodriguez-Sastre N, Siggers T, 
            Gilmore TD
  TITLE     Comparison of NF-kappaB from the protists Capsaspora owczarzaki and 
            Acanthoeca spectabilis reveals extensive evolutionary 
            diversification of this transcription factor.
  JOURNAL   Commun Biol. 2021 Dec 16;4(1):1404. doi: 10.1038/s42003-021-02924-2.
  PUBMED    34916615
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-16"; volume: "4(1):1404. doi"; pages: " 
            10.1038/s42003-021-02924-2"
COMMENT     Sequence Label: pcDNA-FLAG-Co-NF-kB
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3667..3685)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3668..3685)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3705..3722)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3711..3935
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3981..4409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4068..4087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4278..4299
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4418..4438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4423..4752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4603..4738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4665..4684
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4819..5613
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4873..4892)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5483..5502
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5787..5908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5824..5843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5878..5897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5957..5973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5957..5973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5970..5992)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5981..5997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6005..6011,6012..6029,6030..6035))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6050..6071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6188..6205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6359..6947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6439..6458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7118..7909,7910..7978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7741..7760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7979..8083)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8158..8177)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8349..8728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8729..8932
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8883..8903
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8977..8996
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8977..8995
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9018..9041
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aatttgtcct tgcaggagca cactgcaaat ctgctcgcta tggatgattc aacaatggca
       61 gctatgatac tgcattctga tggactgtca ttgttcagag atatgggtct gtacaattcc
      121 gtctctacga gcctggatat tagcggtatt ccaaaatttc cgccaccgcc acagcagccg
      181 caacttgcac aagcgccgcc tcggagaggt cacaaccaat ccagcagtag cgatagccat
      241 agcacaccat cacccggttc cgtgctcttc tccccgagcc cggcttctca agatatgagc
      301 ctccagagtc cggagctggg cctcgttggg cttactggca acagtaggct ggcgtcaacg
      361 tcagaggccg atgatattct gcttgcgaat atactggggc acccagtacg gtcagttagt
      421 gcaaatacat caatggttgg tcttccggat gacctgggat tctcccccac gaccggaatg
      481 gatctcacaa tcacggcgac gagtccagcg acggcagact catccgcgtc agctacagcc
      541 ttccctgcgg cgtccccgat agcttcacct agcgtctcca ccagctccgg gcctgtaact
      601 gttggcggaa ccagcgcggc cttggccgca ttgactccgg acagaattaa taggcttctt
      661 agcgcagccg aggcggcagc cgaaggggcg gccaccctcg ttgaggacct tcttatggtc
      721 acggaggagc ccgcgcagtt cgcccgattc cgctacatga gcgagcagcg agagagatct
      781 ttggccggcg agaactcctt ccccacgctt atggtaaacc ctaagtatgc cagagtggtc
      841 cccgagatgg ccctcgtaac tgctgtcctg gtcacaaaaa tgccggatcc ccacactggg
      901 aggcagcaga agcattggca tcaccttggg ggcatacctg cggctcctct ggaagggcct
      961 caacgcatag caaggttcga caacattgct gtaataatgg acaaggcgaa caataaggat
     1021 aaggacaaat caaaggcacc cgtaagatct aaagacgatc agcgatgtgt caggataatg
     1081 tttgaacttg tctttgtgtc aggtaataca cagttctatg gcagggcaat cagccaacca
     1141 atctacaacg ccaaattggc catcacaaag attagtcata gcagcgggcc cgtgaccggt
     1201 ggtaacgagg ttatcatgtt gtgcagtaag atcagaaaag gcgtgaccgg ggtacggatg
     1261 accgatccaa cccaatggtc agtccaggca cctagcggct ctgcgtggga gttgaatcca
     1321 caaacgctga aggccgactg caatgtaccg ggcgctaatc tttttttcca tcatcaatat
     1381 gcagtcgtcc tgacgctgcc gccctaccac acgcagacca taacagcccc tgtcaccgtc
     1441 aggatctcaa tcttggacac tgacgatgag acagagagtc aatacgttga gtatacctat
     1501 ttgccggccg aagcggcggt gcgcaacgca gagctggcgg ctcggaaacg gagacgggat
     1561 gattcaatga gagattttat ggaccgcttt gatgggtccg acggtggaaa tggctctggg
     1621 agtggccgag gtaacaacgg aggacacgat ggctctgacg ctaacaataa cggccgaggc
     1681 ggtggcggtg ggagttcttc aagtaaaggc ggggacgaac cgtttaattt taattcactc
     1741 attccaatgc accaacacaa gcttcatcaa ctggcgctct ccacggtaag ggctgtgcaa
     1801 gggttcgcgg ccagtgggga tgcacggtat ctgttggcct tgcatagaca gttgcttgca
     1861 gctccgaacg aaaacggtga ctccccgctg cacacagcag tagcacaggg taatctcaga
     1921 tctactatgg cattgctccc attgcttgcg gcagaagatc tccaaagtgt gaacgacatg
     1981 ggggaaactg tcttgcatag cgctgtaatt gagaaaaggg cagccatagc tcggctgctt
     2041 ctggtcgctg gcgcagatct tggccagagt aatgcacgga actttaatcg aaacagcctt
     2101 cactaccttg cccggcatgg agatagggcg actgcaatgg ctgtgttcgg cgtttttggc
     2161 agtgcgcaag ccccaccagc taacacgaac acgcccgcac aggcaccggc aggcgaaacg
     2221 aagccgaaac cagctgactt gagactgttg gcaaggattc aggcgcaggc tataaaagcg
     2281 ctcctggcct gtgaactcga aactggggcc actcccgccc atttggcgat acggggtggc
     2341 cactggcacg tctttgaagc gtgtgctaag ttggcggcat cagcacccat acccaaagcc
     2401 gctggttctc ttctttctat ggtcgctgaa aagagtagtg gacatagtct gttgcactcc
     2461 tgcgtcctgg ccaataatga gcaggctgtt cgacttctta taaatctggg ggcctctggc
     2521 aacgctaggg acttcgggaa gaacacgcca cttcacctgg ccgcacgaca aggacacatc
     2581 ggcatagccg cgctgttggt agaggcaggg gctaccctga gtcttaacgc cgttagccag
     2641 actcccctcg atgttttgac ttcagagggt tccggattga gcagagatca gctccgggca
     2701 ttggtagcag tcctccgagg cgagctcaaa tatgccgaca tgcgcggccg gccaaccctc
     2761 cgaatgccga ctcacgcgga gctgcatagc acggcggccg cactcacttc cgcctcccca
     2821 ggggcagtct cactggctga tttctacgcg ggcaaaaagg cctcaaggag ccccgcccct
     2881 ctcggtgctt cctcaagtct tcttagtagt actggagctt ctgcggctgg cgcctctgcg
     2941 cccacgatag cggcggtgca tgccgcgagc gcaacaccgg tagagcgcac cagcatgaac
     3001 aatgacgatg attacgttct ccttgagaag gacgcgcctt accctgttga gcagcagcct
     3061 cacgggaagc gcaacaagca ttctcaccac agattcaccc ggtccagtca cggctcacag
     3121 gataaagatg agcttaaaaa agataaggat gacccgaaaa aagaaaaaga gccgaaggaa
     3181 ctgtcaaaat tcacactgaa agaggcgttt gtggacggta cgaatttttg ggaacttact
     3241 cggaaattcg ctgggaagaa gaaaatggct tcagcaagta ctggcgagat ggagccgttg
     3301 agtccggaac gaccactcag tccgaccaat gcgggaagtg gggctgcctc accttttaac
     3361 caagctaagg aacaagttag ccccggtgct gtacctccaa caggtcttga gaagcttgtc
     3421 aacaagctca tggacgcttc agaggctacc ctctcaagtc aaccagctga ggccgtgacg
     3481 cccgaacaga aactggctga aaaacttgaa aaattgggac tggcacctgc gtctaccact
     3541 agtgcgccgc caccgcaccc aaaagttgct gctctgaatg cgcagtctgt cgaagatgcg
     3601 cggaagactt ctacccatgc cctttactcc gttgattaac tcgagcatgc atctagaggg
     3661 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc
     3721 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3781 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3841 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3901 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     3961 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4021 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4081 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4141 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4201 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4261 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4321 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4381 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     4441 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4501 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4561 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4621 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4681 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4741 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     4801 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4861 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4921 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4981 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5041 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5101 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5161 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5221 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5281 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5341 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     5401 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5461 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     5521 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5581 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5641 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5701 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5761 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     5821 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5881 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5941 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6001 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6061 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6121 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6181 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6241 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6301 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6361 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6421 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6481 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6541 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6601 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6661 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6721 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6781 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6841 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6901 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6961 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7021 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7081 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7141 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7201 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7261 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7321 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7381 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7441 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7501 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7561 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7621 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7681 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7741 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7801 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7861 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7921 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7981 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8041 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8101 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     8161 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     8221 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     8281 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     8341 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     8401 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     8461 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     8521 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     8581 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     8641 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     8701 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     8761 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     8821 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     8881 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
     8941 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     9001 ccaagcttgg taccatggac tacaaggacg acgatgacaa gttaacacgt gctgcagatg
     9061 cgaattcaga cctttctgaa ctttccggat gggacccg
//
