LOCUS       sequence-356618-        7392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmMextli-M605D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Peter D, Weber R, Kone C, Chung MY, Ebertsch L, Truffault V, 
            Weichenrieder O, Igreja C, Izaurralde E
  TITLE     Mextli proteins use both canonical bipartite and novel tripartite 
            binding modes to form eIF4E complexes that display differential 
            sensitivity to 4E-BP regulation.
  JOURNAL   Genes Dev. 2015 Sep 1;29(17):1835-49. doi: 10.1101/gad.269068.115. 
            Epub 2015 Aug 20.
  PUBMED    26294658
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.269068.115"; journalName: "Genes Dev"; date: 
            "2015-09-1- 1"; volume: "29"; issue: "17"; pages: "1835-49"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmMextli-M605D_AB
FEATURES             Location/Qualifiers
     source          1..7392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2530..2595
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2620..2646
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4643..4684
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4694..4711
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4734..4751)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4784..4918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4808..4827)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4862..4881
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5519..6107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5599..5618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6278..7069,7070..7138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6901..6920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7139..7243)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7311..7329
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccaaca tggacgcaca aacacgacga cgtgagcgtc gcgctgagaa acaagctcaa
     2581 tggaaagctg caaacggtgg atctcctcca catatggctt acccatacga tgttccagat
     2641 tacgctcctc caagcttgaa ttctatggct cacacacacc tgggacgcgc cgtgaaaaac
     2701 attgaggcgc cacgcccgtt gaagacacaa tcgcgatcgt cgttgaagaa cagctacctg
     2761 gtgatcgagg agctaatcca gttgatagac aacgtgaccg tcggtcttca gtcgtgcaac
     2821 accacgccgg aatcgatcac gctgctcctg cacaacctcc gggtgcatgg accgcaactg
     2881 gaggcggtgt ccaaggatac actggaccgg gccttcgtcg ttttccgcaa cgcttcgcag
     2941 gacgaacggc tgaatataac gacgcgcctc aagctgctcg aactcatcga gctgcgcgcc
     3001 aagagctggg acaatgacga cacaatcgcg tactacaagt ccaagcagca gatctccaac
     3061 gtggagctgc cttcggaata ccagtacgat gccggcgtgc agcctggcgc cttcagcacc
     3121 tcccccacat tcggcgtcag cggcggtgtt ggtggcgtta acgatggagc cgcagccgcc
     3181 gctgccgccg tcttcaatgc agcatctgca gctgcagccg cccaagctgc ggccattgcg
     3241 gccgtgggca catccaacca gcagcacatg ctgctgccgc ccggtgaggt cattcgtaat
     3301 tcgggcaagt tccccaaacc gaccaagatt cctggcaaga cgtactgcaa ggacgaggtc
     3361 gtgattcgca atgctgactc cggcaaagta atgggcatca aggggcgtcg cgtgcacatg
     3421 atcgaggagc taagcgaaac cattatatcg tttcaaagag tcaacccggg tgccaaggag
     3481 cgtttggtgc agattactgg tccagctgag gataaaatta actatgccaa acaactgatg
     3541 gaggacacca ttcgtcggaa cgcctcgccg gtgcgcctgg aacccgctcc tgccgttgga
     3601 ggatcctgct cctcgcttaa ctcgtccaac tcggacgacg ccattgtgca accaagaacg
     3661 ccaaccggaa gcagtttggc caaccggcta agcttcaact cggcccaaaa cttcatgacc
     3721 gccaccgctg ctgcacagca gatctcgcag cagatgcacc accaaacgca tcacctgcag
     3781 caccaacagc agcagcaggt cgccgcagta gccgccgcag cagcagcggc ggcacatgct
     3841 caggcgacag ccgctgccgg taaagtcttg cgccccaatc aacagctgct aatgcactca
     3901 tattccacaa acgatgcttc cgtaggtgaa tacaagttca cggtcaacgt gggccagcac
     3961 ctgatcaaga tcaccggcga ctgctgcgaa ctagtgcgcg tggccaaact cgtgctcgat
     4021 gactatttta gcagctcgga gtttctggca tccattgagg ctggcgccgc cttcgacggc
     4081 acttcgctgg tgaccacgcc atcgactccg cttcccggag ccgggccgcc gcaattttgg
     4141 ctgcccggca cggacagtgg cattggactg aattgcgttg tttcctcgtc agcgaacaac
     4201 aatggtgaag gcgacgacga ggtgttcgct gagcccagca atggaggatc atcgactagc
     4261 aatcagaatg gactggccag atcgcgtcgc agccactttt cgcgcaagga gtccacgccg
     4321 gagaccaagg gcgctcggga aaagggtgac ttggacgatt tggcaggcac caattccttg
     4381 aaaagcaatg catctcgcgt ttcttacgat attgaacact tgctctacta ctccatgagt
     4441 ccgcactcgt ggaccctgcc caccgactgg caaaaggacc aggagacagc gccctcgatt
     4501 ctgcgcaaca aggatctgca ggatgagagc cagcgcttcg atggtgataa gtatctggcc
     4561 agcattaaga cggccgccaa gcgagacatt gtggctgccg atgaggtcga gactctggac
     4621 gaataagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     4681 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
     4741 tgtgccttct aaggcctgag ctcgctgatc agcctcgatc gaggatccag acatgataag
     4801 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     4861 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4921 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     4981 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagtcga ccgatgccct
     5041 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     5101 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
     5161 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
     5221 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
     5281 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
     5341 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     5401 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     5461 gacagcttca aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5941 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     6241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     6601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     6901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     7261 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     7321 tcacgaggcc ctttcgtctt caagaattaa ttctatattc taaaaacaca aatgatactt
     7381 ctaaaaaaaa at
//
