LOCUS       sequence-356623-        6339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1674-1962-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Kuzuoglu-Ozturk D, Bhandari D, Huntzinger E, Fauser M, Helms S, 
            Izaurralde E
  TITLE     miRISC and the CCR4-NOT complex silence mRNA targets independently 
            of 43S ribosomal scanning.
  JOURNAL   EMBO J. 2016 Jun 1;35(11):1186-203. doi: 10.15252/embj.201592901. 
            Epub 2016 Mar 23.
  PUBMED    27009120
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201592901"; journalName: "EMBO J"; date: "2016-06-1- 
            1"; volume: "35"; issue: "11"; pages: "1186-203"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC
FEATURES             Location/Qualifiers
     source          1..6339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1462..1527
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1552..1578
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2522..2563
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2573..2590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2613..2630)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2663..2797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2687..2706)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2741..2760
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3398..3986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3478..3497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4157..4948,4949..5017))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4780..4799
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5018..5122)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5190..5208
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(5272..6339,1..1442)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 accgcatgtt gccgtttcct tttatgcgtc attttggctc gaaataggca attatttaaa
       61 caaagattag tcaacgaaaa cgctaaaata aataagtcta caatatggtt acttattgcc
      121 atgtgtgtgc agccaacgat agcaacaaaa gcaacaacac aggtggcttt ccctctttca
      181 ctttttgttt gcaagccgcg tgcgagcaag acggcacgac cggcaaacgc aattacgctg
      241 acaaagagca gacgaagttt tggcgaaaaa catcaaggcg cctgatacga atgcatttgc
      301 aataacaatt gcgatattta atattgttta tgaagctgtt tgacttcaaa acacacaaaa
      361 aaaaaaataa aacaaattat ttgaaagaga attaggaatc ggacgcttat cgttagggta
      421 acaacaagaa atgcttactg agtcacagcc tctggaaaac tgccgcaagc cagagagaga
      481 gagaaaaaga gggagagcag cttagaccgc atgtgcttgt gtgtgaggcg tctctctctt
      541 cgtctctgtt gcgcaaacgc atagactgca ctgaaaaaat cgattaccta ttttttatga
      601 atgaatattt gcactattac tattcaaaac tattaagata gcaatcacat tcaatagcca
      661 aatactatac cacctgagcg atgcaacgaa atgatcaatt tgagcaaaaa tgctgcatat
      721 ttaggacggc atcattatag aaatgcttct tgctgtgtac ttttctctcg tctggcagct
      781 gtttcgccgt tattgttaaa accggcttaa gttaggtgtg ttttctacga ctagtgaatg
      841 ccctactaga agatgtgtgt tgcacaaaat gtccctggaa taaccaattt gaagtgcaga
      901 tagcagtaaa cgtaagctaa tatgaatatt atttaactgt aatgttttaa tatcgctgga
      961 cattactaat aaacccacta taaacacatg tacatatgta tgttttggca tacaatgagt
     1021 agttggggaa aaaatgtgta aaagcaccgt gaccatcaca gcataaagat aaccagctga
     1081 agtatcgaat atgagtaacc cccaaattga atcacatgcc gcaactgata ggacccatgg
     1141 aagtacactc ttcatggcga tatacaagac acacacaagc acgaacaccc agttgcggag
     1201 gaaattctcc gtaaatgaaa acccaatcgg cgaacaattc atacccatat atggtaaaag
     1261 ttttgaacgc gacttgagag cggagagcat tgcggctgat aaggttttag cgctaagcgg
     1321 gctttataaa acgggctgcg ggaccagttt tcatatcact accgtttgag ttcttgtgct
     1381 gtgtggatac tcctcccgac acaaagccgc tccatcagcc agcagtcgtc taatccagag
     1441 accccggatc ggggtaccaa catggacgca caaacacgac gacgtgagcg tcgcgctgag
     1501 aaacaagctc aatggaaagc tgcaaacggt ggatctcctc cacatatggc ttacccatac
     1561 gatgttccag attacgctcc tccaagcttg aattctgcag atatccagca cagtggcggc
     1621 cgctcgagac cagctacact ttatgctgtg tactcggagt tcgctagaag catacctggc
     1681 ttccagcaga tgagcgatcg cgatattgcc ctgtttgtgc ccaagccgac ggatttgtct
     1741 cagccaaatg tgtttgctaa tgatgatagc atggtatacg gggaactggc tagcaaaatg
     1801 gaggccttca tgaacacggc aatcggagtg ccgacactcc agatccaggc tagcaagatg
     1861 cacatgctac tcaacgccct gatagcgacg cgtcgactgc gtgaccaaga gtctgccttt
     1921 aatctgctga cccgcgcggt cgagggtttg accgaaggat tggtcaatat gcatgaaaac
     1981 atggagcaaa tgaagatgta ccaaaacatc catctccgta tccttggctt gctgaacaac
     2041 agtttcggtg ctccaaacac agagcgagcg gtaacaaagt gtttcttcga tatccgagag
     2101 gaggtgcgat ataacgtgga ggcagctcgc gctttgatta cgtcgcactt tgtcaatctg
     2161 aatcagtttg acggaatgct acgcgactgc atggacaacg gaaacaatta tgtggctata
     2221 tcgttcggta tcgcactgct ggagcgcctc atcatggacg atcgagtgat taatattgtt
     2281 tcggacaacg aattcatggc aactgttgag ctgctaggta ggctgaccca acaccgtcac
     2341 cgctaccccg agtgtatcgt caacgccatc gacaccctgt ggtccggcaa tttcaatagc
     2401 tcctccgatt attccccctt taatggaaac gaccgctatc tgagcggcgc tagccactat
     2461 attcatagcg gaatgcacca tgtccgctcg tgtgacttat ctagagggcc cgcggttcga
     2521 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
     2581 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc
     2641 tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca
     2701 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2761 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2821 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2881 atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca
     2941 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     3001 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     3061 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     3121 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     3181 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     3241 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     3301 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggccagcaaa
     3361 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3901 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3961 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4021 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4081 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4141 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4201 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4261 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4321 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4381 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4441 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     4501 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4561 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4621 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4681 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4741 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     4801 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4861 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4921 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4981 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5041 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5101 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     5161 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     5221 aagaattaat tctatattct aaaaacacaa atgatacttc taaaaaaaaa tcatgaatgg
     5281 catcaactct gaatcaaatc tttgcagatg cacctacttc tcatttccac tgtcacatca
     5341 tttttccaga tctcgctgcc tgttatgtgg cccacaaacc aagacacgtt ttatggccat
     5401 taaagctggc tgatcgtcgc caaacaccaa atacataatg aatatgtaca cattcgagaa
     5461 agaagcgatc aaagaagcgt cttcgggcgg agtaggagaa tgcggaggag aaggagaacg
     5521 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
     5581 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
     5641 cgttacccgg ggccattttc aaagactcgt cggcaagata agattgtgtc actcgctgtc
     5701 tctcttcatt tgtcgaagaa tgctgaggaa tttcgcgatg acgtcggcga gtattttgaa
     5761 gaatgagaat aatttgtatt tatacgaaaa tcagttagtg gaattttcta caaaaacatg
     5821 ttatctatag ataattttgt tgcaaaatat gttgactatg acaaagattg tatgtatata
     5881 cctttaatgt attctcattt tcttatgtat ttataatggc aatgatgata ctgatgatat
     5941 tttaagatga tgccagacca aaaggcttga atttctgcgt cttttgccga acgcagtgca
     6001 tgtgcaattg ttgttttttg gaatattcaa ttttcggact gtccgctttg atttcagttt
     6061 cttggcttat tcaaaaagca aagtaaagcc aaaaaagcga gatggcaata ccaaatgcgg
     6121 caaaacggta gtggaaggaa aggggtgcgg ggcagcggaa ggaagggtgg ggcggggcgt
     6181 ggcggggtct gtggctgggc gcgacgtcac cgacgttgga gccactcctt tgaccatgtg
     6241 tgcgtgtgtg tattattcgt gtctcgccac tcgccggttg tttttttctt tttatgctgc
     6301 gctctctcta gcgccatctc gcttacgcat gctcaacgc
//
