LOCUS       sequence-356652-       18124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18124)
  TITLE     Daigle Lab TIGRE3.0 plasmids 2022
REFERENCE   2  (bases 1 to 18124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1205-Ai227 (TICF-nls-EGFP-ICF-tdTomato-WPRE)
FEATURES             Location/Qualifiers
     source          1..18124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        354..401
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             420..1223
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(483..502)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1093..1112
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1252..1274
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1266..1283
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1267..1283
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1427..1476
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     enhancer        4699..5002
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5004..5281
                     /label=chicken beta-actin promoter
     intron          5282..6298
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     polyA_signal    6471..6519
                     /note="synthetic polyadenylation signal"
     polyA_signal    6528..7004
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    7083..7131
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             7347..7367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7368..7370,7371..8084)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7368..7370,7371..8081)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     8018..8039
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8098..8305
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        10829..11132
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11134..11411
                     /label=chicken beta-actin promoter
     protein_bind    12517..12550
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    12596..12644
                     /note="synthetic polyadenylation signal"
     polyA_signal    12653..13129
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    13208..13256
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    13416..13449
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      13474..13483
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(13613..13631)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     13881..13900
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     14075..14098
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    14943..15531
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14996..15016)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15414..15425)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15556..15763
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    15777..15810
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        15837..16336
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(15872..15895)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     16269..16288
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    17028..17077
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(17390..17978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17470..17489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
       61 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      121 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
      181 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      241 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      301 ttcgccagtt aatagtttgc gcaacgtttt ggcggtttaa acattaagca cgtgttgaca
      361 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
      421 tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
      481 ggctattcgg ctatgactgg gcacaacaga cgatcggctg ctctgatgcc gccgtgttcc
      541 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
      601 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
      661 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
      721 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
      781 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
      841 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
      901 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
      961 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     1021 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     1081 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     1141 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     1201 gccttcttga cgagttcttc tgaagtgtta actgggtaac gccagggttt tcccagtcac
     1261 gacgttgtaa aacgacggcc agtgaattcg agctcggtac cgaaaaaccg ctcagcggcg
     1321 cccgcaccca aacgaaaaca cccccggccc aagcggaccg ggggtgtcgc gtgaaatgga
     1381 tcagtgagcg tggccgtggt ggtggccgtg gccgtgctcg tcctcgtcgg ccggcttgtc
     1441 gacgacggcg gtctccgtcg tcaggatcat ccgggccacc gaggcggcgt tgagaacagc
     1501 cgagcgggtc accttggccg gatcgacgac gccggcggag accagatcac cgaactccag
     1561 cgtggcggcg ttgaagccct gccccttggg aagttcggag atttcggccg gccaatcgat
     1621 tttaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1681 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1741 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1801 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1861 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1921 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1981 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2041 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2101 cgccacaaca tcgaggacgg ttaattaaga attcgagctc gctagagccc catcctcact
     2161 gactccgtcc tggagttgga tgagagataa tggccttacg ttgtgccagg ggagggtcgg
     2221 gctggattta gcaagattta ccttctccaa agagcggtgc tgcagtggca cagctgccca
     2281 cggaggtggg ggggtcaccg tccctggagg tgatgaagaa ctgtggggat gtggcactga
     2341 gggacatggc cagtgggcac ggtgggtggg ttggggttgg tcttggggat cttggagggc
     2401 ttttccagcc ttcatgattt gacgattgta tgaacatcta catggcaatt ctccagctgc
     2461 ctgtcccagt cctactgacc cagctgtatc tctccaggca agctcttcca ccccttctgc
     2521 ttgcatccag acaccatcaa acatgcaggc tcagacacag ggaccagcag tgtctgtggc
     2581 ctttttgtgc tcctctccat gctgggtttt aacttgctct ttgtccttct atcctatctt
     2641 cttatcctta aggctgttct gaacgctgtg acttggagag tgtcccagag ccctcaacac
     2701 ctgcatgtcc cacgtccatg ctgtcctgca cttccttatc cccaagatct gcctctccgt
     2761 gatgcactga attggcaaac atgtgtcacc ccagaccaac aatgtcacag caaactcccc
     2821 cttgatagga caagggggaa tggctttaca ctgagacagg ggaggtttgg gttggatatg
     2881 aggaggcagt ttttccccca gagggtggtg acgcactgaa caggttgccc aaggaggctg
     2941 tggatgcccc atccctgcag gcattcaagg ccaggctgga tgtggctctg ggcagcctgg
     3001 gctgctggtt gatgaccctg cacatagcag ggggttggat ctggatgagc actgtgctcc
     3061 tttgcaaccc aggccgttct atgattctgt cattctaaat ctctctttca gcctaaagct
     3121 ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg
     3181 aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg
     3241 gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc
     3301 gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc tctagcgagc
     3361 tcggtaccca gagccccatc ctcactgact ccgtcctgga gttggatgag agataatggc
     3421 cttacgttgt gccaggggag ggtcgggctg gatttagcaa gatttacctt ctccaaagag
     3481 cggtgctgca gtggcacagc tgcccacgga ggtggggggg tcaccgtccc tggaggtgat
     3541 gaagaactgt ggggatgtgg cactgaggga catggccagt gggcacggtg ggtgggttgg
     3601 ggttggtctt ggggatcttg gagggctttt ccagccttca tgatttgacg attgtatgaa
     3661 catctacatg gcaattctcc agctgcctgt cccagtccta ctgacccagc tgtatctctc
     3721 caggcaagct cttccacccc ttctgcttgc atccagacac catcaaacat gcaggctcag
     3781 acacagggac cagcagtgtc tgtggccttt ttgtgctcct ctccatgctg ggttttaact
     3841 tgctctttgt ccttctatcc tatcttctta tccttaaggc tgttctgaac gctgtgactt
     3901 ggagagtgtc ccagagccct caacacctgc atgtcccacg tccatgctgt cctgcacttc
     3961 cttatcccca agatctgcct ctccgtgatg cactgaattg gcaaacatgt gtcaccccag
     4021 accaacaatg tcacagcaaa ctcccccttg ataggacaag ggggaatggc tttacactga
     4081 gacaggggag gtttgggttg gatatgagga ggcagttttt cccccagagg gtggtgacgc
     4141 actgaacagg ttgcccaagg aggctgtgga tgccccatcc ctgcaggcat tcaaggccag
     4201 gctggatgtg gctctgggca gcctgggctg ctggttgatg accctgcaca tagcaggggg
     4261 ttggatctgg atgagcactg tgctcctttg caacccaggc cgttctatga ttctgtcatt
     4321 ctaaatctct ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc
     4381 aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg
     4441 ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc
     4501 cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt
     4561 gggggggggc tgtccctcta ggggtacccg gggatccacc ggtccttaat taaccgttta
     4621 aacaattctg caggaatcta gttattaata gtaatcaatt acggggtcat tagttcatag
     4681 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4741 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4801 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4861 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4921 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     4981 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     5041 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     5101 gatgggggcg gggggggggg gggggcgcgc gccrggsggg gsggggsggg gsgrggggsg
     5161 gggsggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     5221 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     5281 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     5341 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     5401 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     5461 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     5521 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     5581 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     5641 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     5701 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     5761 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     5821 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     5881 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     5941 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     6001 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     6061 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     6121 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     6181 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     6241 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     6301 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgatttgata
     6361 ccatttaaat ggggatcaaa ttcaattaaa tgaagttcct attcttcaaa aggtatagga
     6421 acttccctca gcaccatggc tagcggcagc ctcggagttt gaatagatag aataaaatat
     6481 ctttattttc attccatctg tgtgttggtt ttttgtgtga gatctacggg tggcatccct
     6541 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     6601 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     6661 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     6721 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     6781 ccgcctcctg ggttcaagcg attctcctgc ctcaggctcc cgagttgttg ggattccagg
     6841 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     6901 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     6961 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgcct cagctaattg
     7021 agtagggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     7081 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     7141 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     7201 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     7261 tcgcagccaa cgtcggggcg gcaggccgtg gaagttccta ttcttcaaaa ggtataggaa
     7321 cttccacgcg tgccgccacc atgccaccca aaaagaaaag aaaggtggtg agcaagggcg
     7381 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     7441 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     7501 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     7561 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     7621 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     7681 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     7741 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     7801 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     7861 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     7921 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     7981 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     8041 ccgccgccgg gatcactctc ggcatggatg agctgtacaa gtaactcgag gcctcgactg
     8101 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8161 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8221 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8281 aagacaatag caggcatgct ggggacacgt gggatcctct agagccccat cctcactgac
     8341 tccgtcctgg agttggatga gagataatgg ccttacgttg tgccagggga gggtcgggct
     8401 ggatttagca agatttacct tctccaaaga gcggtgctgc agtggcacag ctgcccacgg
     8461 aggtgggggg gtcaccgtcc ctggaggtga tgaagaactg tggggatgtg gcactgaggg
     8521 acatggccag tgggcacggt gggtgggttg gggttggtct tggggatctt ggagggcttt
     8581 tccagccttc atgatttgac gattgtatga acatctacat ggcaattctc cagctgcctg
     8641 tcccagtcct actgacccag ctgtatctct ccaggcaagc tcttccaccc cttctgcttg
     8701 catccagaca ccatcaaaca tgcaggctca gacacaggga ccagcagtgt ctgtggcctt
     8761 tttgtgctcc tctccatgct gggttttaac ttgctctttg tccttctatc ctatcttctt
     8821 atccttaagg ctgttctgaa cgctgtgact tggagagtgt cccagagccc tcaacacctg
     8881 catgtcccac gtccatgctg tcctgcactt ccttatcccc aagatctgcc tctccgtgat
     8941 gcactgaatt ggcaaacatg tgtcacccca gaccaacaat gtcacagcaa actccccctt
     9001 gataggacaa gggggaatgg ctttacactg agacagggga ggtttgggtt ggatatgagg
     9061 aggcagtttt tcccccagag ggtggtgacg cactgaacag gttgcccaag gaggctgtgg
     9121 atgccccatc cctgcaggca ttcaaggcca ggctggatgt ggctctgggc agcctgggct
     9181 gctggttgat gaccctgcac atagcagggg gttggatctg gatgagcact gtgctccttt
     9241 gcaacccagg ccgttctatg attctgtcat tctaaatctc tctttcagcc taaagctttt
     9301 tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa
     9361 gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat
     9421 gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg
     9481 ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctct agagccccat
     9541 cctcactgac tccgtcctgg agttggatga gagataatgg ccttacgttg tgccagggga
     9601 gggtcgggct ggatttagca agatttacct tctccaaaga gcggtgctgc agtggcacag
     9661 ctgcccacgg aggtgggggg gtcaccgtcc ctggaggtga tgaagaactg tggggatgtg
     9721 gcactgaggg acatggccag tgggcacggt gggtgggttg gggttggtct tggggatctt
     9781 ggagggcttt tccagccttc atgatttgac gattgtatga acatctacat ggcaattctc
     9841 cagctgcctg tcccagtcct actgacccag ctgtatctct ccaggcaagc tcttccaccc
     9901 cttctgcttg catccagaca ccatcaaaca tgcaggctca gacacaggga ccagcagtgt
     9961 ctgtggcctt tttgtgctcc tctccatgct gggttttaac ttgctctttg tccttctatc
    10021 ctatcttctt atccttaagg ctgttctgaa cgctgtgact tggagagtgt cccagagccc
    10081 tcaacacctg catgtcccac gtccatgctg tcctgcactt ccttatcccc aagatctgcc
    10141 tctccgtgat gcactgaatt ggcaaacatg tgtcacccca gaccaacaat gtcacagcaa
    10201 actccccctt gataggacaa gggggaatgg ctttacactg agacagggga ggtttgggtt
    10261 ggatatgagg aggcagtttt tcccccagag ggtggtgacg cactgaacag gttgcccaag
    10321 gaggctgtgg atgccccatc cctgcaggca ttcaaggcca ggctggatgt ggctctgggc
    10381 agcctgggct gctggttgat gaccctgcac atagcagggg gttggatctg gatgagcact
    10441 gtgctccttt gcaacccagg ccgttctatg attctgtcat tctaaatctc tctttcagcc
    10501 taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
    10561 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
    10621 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
    10681 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctct
    10741 agagtcgacg gcgcgccctg caggaatcta gttattaata gtaatcaatt acggggtcat
    10801 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
    10861 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
    10921 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
    10981 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
    11041 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
    11101 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
    11161 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
    11221 tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg
    11281 gcgaggggcg gggcggggcg aggcggagag gkgcggcggc agccaatcag agcggcgcgc
    11341 yccgaaagtt tccttttwtg gcgaggsggc ggcggcggcg gccctataaa aarcgaagcg
    11401 cgcggsgggs ggggrgkcgc tgcgcgcygc cttcgccccg tgccccgctc cgccgccgcc
    11461 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
    11521 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
    11581 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
    11641 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
    11701 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
    11761 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
    11821 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
    11881 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
    11941 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
    12001 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
    12061 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
    12121 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
    12181 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
    12241 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
    12301 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
    12361 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
    12421 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
    12481 ttgatttgat accgcgggcc ccgcgtggac ggtaccgaag ttcctattct ctagaaagta
    12541 taggaacttc cctcagcacc atggctagcg gcagcctcgg agtttgaata gatagaataa
    12601 aatatcttta ttttcattcc atctgtgtgt tggttttttg tgtgagatct acgggtggca
    12661 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
    12721 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
    12781 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
    12841 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
    12901 aatctccgcc tcctgggttc aagcgattct cctgcctcag gctcccgagt tgttgggatt
    12961 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
    13021 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
    13081 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgcctcagct
    13141 aattgagtag gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
    13201 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
    13261 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
    13321 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
    13381 agggctcgca gccaacgtcg gggcggcagg ccgtggaagt tcctattctc tagaaagtat
    13441 aggaacttca cgcgcgcgtc catttaattc gccgccacca tggtgagcaa gggcgaggag
    13501 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
    13561 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
    13621 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
    13681 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
    13741 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
    13801 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
    13861 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
    13921 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
    13981 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
    14041 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
    14101 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg
    14161 catggtaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac
    14221 atggccgtga ttaaggagtt tatgaggttt aaagtcagga tggagggcag catgaacggg
    14281 cacgagtttg agattgaagg agagggggaa gggaggccct atgagggcac ccagacagca
    14341 aagctgaaag tgacaaaggg cggccctctg ccattcgcat gggacatcct gtctcctcag
    14401 tttatgtatg gaagtaaagc ctacgtgaaa caccctgctg acattccaga ttacaagaaa
    14461 ctgagcttcc cagaggggtt taagtgggaa agagtgatga acttcgaaga cgggggcctg
    14521 gtgactgtca cccaggacag ctctctgcag gacggcactc tgatctataa ggtgaaaatg
    14581 cgcggcacca attttccccc tgatggccca gtcatgcaga agaagacaat gggctgggag
    14641 gcttcaacag aaaggctgta ccccagagac ggcgtgctga agggagagat ccaccaggca
    14701 ctgaagctga aagatggagg gcattatctg gtcgaattca aaaccatcta catggccaag
    14761 aaacccgtgc agctgcctgg ctactattac gtcgacacaa agctggatat tactagccat
    14821 aacgaagact acaccattgt ggagcagtat gagcggagcg aggggaggca ccatctgttt
    14881 ctgtacggca tggacgagct gtacaagtaa ctaaggttaa ccgttacgta aagcttgtcg
    14941 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    15001 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    15061 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    15121 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    15181 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    15241 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    15301 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    15361 tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    15421 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    15481 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgacacc
    15541 gcaattgtgc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    15601 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    15661 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    15721 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggacacgtgg gatccggtgc
    15781 cagggcgtgc ccttgggctc cccgggcgcg tttgggcggc cgcatcgaat tctaccgggt
    15841 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
    15901 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatcctccg gtaggcgcca
    15961 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
    16021 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
    16081 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
    16141 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
    16201 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
    16261 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
    16321 atctccgggc ctttcgacct gcaatcgccg ctagccgcca ccatgaaaaa gcctgaactc
    16381 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
    16441 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
    16501 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
    16561 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
    16621 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
    16681 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
    16741 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
    16801 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
    16861 gacgacaccg tcagtgtaag tgctagcccc taggcctgaa tcgaaaaacc gctcagcggc
    16921 gcccgcaccc aaacgaaaac acccccggcc caagcggacc gggggtgtcg cgtgaaatgg
    16981 atcagtgagc gtggccgtgg tggtggccgt ggccgtgctc gtcctcgtcg gccggcttgt
    17041 cgacgacggc ggtctccgtc gtcaggatca tccgggccac cgaggcggcg ttgagaacag
    17101 ccgagcgggt caccttggcc ggatcgacga cgccggcgga gaccagatca ccgaactcca
    17161 gcgtggcggc gttgaagccc tgccccttgg gaagttcgga gagagctcgt cgactgcaga
    17221 ggcctgcatg caagcttggc gtaatcatgg tcatagctgt ttccttatca gctcactcaa
    17281 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    17341 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    17401 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    17461 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    17521 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    17581 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    17641 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    17701 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    17761 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    17821 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    17881 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    17941 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    18001 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    18061 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    18121 gtat
//
