LOCUS       sequence-356654-       18245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18245)
  TITLE     Daigle Lab TIGRE3.0 plasmids 2022
REFERENCE   2  (bases 1 to 18245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP993-Ai195 (TIT2L-GC7s-ICF-IRES-tTA2)
FEATURES             Location/Qualifiers
     source          1..18245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        456..503
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             522..1325
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(585..604)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1195..1214
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1354..1376
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1368..1385
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1369..1385
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1529..1578
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    join(4751..4769,4770..4792,4793..4811,4812..4834,4835..4853,
                     4854..4876,4877..4895,4896..4918,4919..4937,4938..4960,
                     4961..4979,4980..5002,5003..5021)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5054..5092
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5089..5113
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(5226..5259)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5336..5384
                     /note="synthetic polyadenylation signal"
     polyA_signal    5393..5869
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    5881..6342
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(6369..6402)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6413..6422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6419..6427,6428..6445,6446..6448,6449..6481,6482..6484,
                     6485..6508,6509..6535,6536..6592,6593..6598,6599..6868,
                     6869..6886,6887..7321,7322..7327,7328..7771)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRVIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKELFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      6515..6524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6805..6826
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7796..8384
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8267..8278)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    8415..8622
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        11146..11449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11451..11728
                     /label=chicken beta-actin promoter
     intron          11729..12745
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     12669..12691
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     12753..12772
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    12833..12866
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    12912..12960
                     /note="synthetic polyadenylation signal"
     polyA_signal    12969..13445
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(13480..13499)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13524..13572
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    13732..13765
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    13788..14361
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    13811..14363
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(13955..13972)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     14182..14201
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(14371..14988,14989..14994,14995..15030,15031..15033,
                     15034..15069,15070..15072,15073..15108,15109..15117)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    15160..15748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(15631..15642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15779..15986
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    16000..16033
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        16060..16559
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(16095..16118)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     16492..16511
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    17251..17300
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(17613..18201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17693..17712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
       61 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      121 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      181 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      241 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      301 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
      361 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
      421 gcgcaacgtt ttggcggttt aaacattaag cacgtgttga caattaatca tcggcatagt
      481 atatcggcat agtataatac gacaaggtga ggaactaaac catgggatcg gccattgaac
      541 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
      601 gggcacaaca gacgatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
      661 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
      721 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
      781 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
      841 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
      901 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
      961 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     1021 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     1081 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     1141 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     1201 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     1261 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     1321 tctgaagtgt taactgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1381 ccagtgaatt cgagctcggt accgaaaaac cgctcagcgg cgcccgcacc caaacgaaaa
     1441 cacccccggc ccaagcggac cgggggtgtc gcgtgaaatg gatcagtgag cgtggccgtg
     1501 gtggtggccg tggccgtgct cgtcctcgtc ggccggcttg tcgacgacgg cggtctccgt
     1561 cgtcaggatc atccgggcca ccgaggcggc gttgagaaca gccgagcggg tcaccttggc
     1621 cggatcgacg acgccggcgg agaccagatc accgaactcc agcgtggcgg cgttgaagcc
     1681 ctgccccttg ggaagttcgg agatttcggc cggccaatcg attttaccgg ggtggtgccc
     1741 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1801 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1861 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1921 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1981 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2041 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2101 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2161 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2221 ggttaattaa gaattcgagc tcgctagagc cccatcctca ctgactccgt cctggagttg
     2281 gatgagagat aatggcctta cgttgtgcca ggggagggtc gggctggatt tagcaagatt
     2341 taccttctcc aaagagcggt gctgcagtgg cacagctgcc cacggaggtg ggggggtcac
     2401 cgtccctgga ggtgatgaag aactgtgggg atgtggcact gagggacatg gccagtgggc
     2461 acggtgggtg ggttggggtt ggtcttgggg atcttggagg gcttttccag ccttcatgat
     2521 ttgacgattg tatgaacatc tacatggcaa ttctccagct gcctgtccca gtcctactga
     2581 cccagctgta tctctccagg caagctcttc caccccttct gcttgcatcc agacaccatc
     2641 aaacatgcag gctcagacac agggaccagc agtgtctgtg gcctttttgt gctcctctcc
     2701 atgctgggtt ttaacttgct ctttgtcctt ctatcctatc ttcttatcct taaggctgtt
     2761 ctgaacgctg tgacttggag agtgtcccag agccctcaac acctgcatgt cccacgtcca
     2821 tgctgtcctg cacttcctta tccccaagat ctgcctctcc gtgatgcact gaattggcaa
     2881 acatgtgtca ccccagacca acaatgtcac agcaaactcc cccttgatag gacaaggggg
     2941 aatggcttta cactgagaca ggggaggttt gggttggata tgaggaggca gtttttcccc
     3001 cagagggtgg tgacgcactg aacaggttgc ccaaggaggc tgtggatgcc ccatccctgc
     3061 aggcattcaa ggccaggctg gatgtggctc tgggcagcct gggctgctgg ttgatgaccc
     3121 tgcacatagc agggggttgg atctggatga gcactgtgct cctttgcaac ccaggccgtt
     3181 ctatgattct gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc
     3241 aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac
     3301 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg
     3361 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta
     3421 catccctggg ggctttgggg gggggctgtc cctctagcga gctcggtacc cagagcccca
     3481 tcctcactga ctccgtcctg gagttggatg agagataatg gccttacgtt gtgccagggg
     3541 agggtcgggc tggatttagc aagatttacc ttctccaaag agcggtgctg cagtggcaca
     3601 gctgcccacg gaggtggggg ggtcaccgtc cctggaggtg atgaagaact gtggggatgt
     3661 ggcactgagg gacatggcca gtgggcacgg tgggtgggtt ggggttggtc ttggggatct
     3721 tggagggctt ttccagcctt catgatttga cgattgtatg aacatctaca tggcaattct
     3781 ccagctgcct gtcccagtcc tactgaccca gctgtatctc tccaggcaag ctcttccacc
     3841 ccttctgctt gcatccagac accatcaaac atgcaggctc agacacaggg accagcagtg
     3901 tctgtggcct ttttgtgctc ctctccatgc tgggttttaa cttgctcttt gtccttctat
     3961 cctatcttct tatccttaag gctgttctga acgctgtgac ttggagagtg tcccagagcc
     4021 ctcaacacct gcatgtccca cgtccatgct gtcctgcact tccttatccc caagatctgc
     4081 ctctccgtga tgcactgaat tggcaaacat gtgtcacccc agaccaacaa tgtcacagca
     4141 aactccccct tgataggaca agggggaatg gctttacact gagacagggg aggtttgggt
     4201 tggatatgag gaggcagttt ttcccccaga gggtggtgac gcactgaaca ggttgcccaa
     4261 ggaggctgtg gatgccccat ccctgcaggc attcaaggcc aggctggatg tggctctggg
     4321 cagcctgggc tgctggttga tgaccctgca catagcaggg ggttggatct ggatgagcac
     4381 tgtgctcctt tgcaacccag gccgttctat gattctgtca ttctaaatct ctctttcagc
     4441 ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
     4501 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
     4561 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
     4621 cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctc
     4681 taggggtacc cggggatcca ccggtcgtaa gcggttataa gctgcaataa acaagtttcg
     4741 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     4801 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     4861 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     4921 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     4981 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg
     5041 gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
     5101 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
     5161 ggaccgatcc agcctccgcg gccccgaatt cgagctcggt acccggggat caaattcaat
     5221 taaatataac ttcgtatagc atacattata cgaagttatt cgcgatgaat aaatgaaagc
     5281 ttgcagatcc cctcagcacc atggctagcg gcagcctcgg agtttgaata gatagaataa
     5341 aatatcttta ttttcattcc atctgtgtgt tggttttttg tgtgagatct acgggtggca
     5401 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     5461 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     5521 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     5581 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     5641 aatctccgcc tcctgggttc aagcgattct cctgcctcag gctcccgagt tgttgggatt
     5701 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     5761 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     5821 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgcctcagcg
     5881 ctgtaagtct gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag
     5941 tttttcctgt catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa
     6001 ggattggagc tacgggggtg ggggtggggt gggattagat aaatgcctgc tctttactga
     6061 aggctcttta ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag
     6121 caaaaccaaa ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct
     6181 ctcgtgggat cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc
     6241 caaatgtgtc agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact
     6301 tcattctcag tattgttttg ccaagttcta attccatcag aaagctttta cttaactgaa
     6361 tagctaacat aacttcgtat agcatacatt atacgaagtt atcacgcgtg ccgccaccat
     6421 gggttctcat catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg
     6481 tcgggatctg tacgacgatg acgataagga tctcgccacc atggtcgact catcacgtcg
     6541 taagtggaat aagacaggtc acgcagtcag agtgataggt cggctgagct cactcgagaa
     6601 cgtctatatc aaggccgaca agcagaagaa cggcatcaag gcgaacttcc acatccgcca
     6661 caacatcgag gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg
     6721 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca aactttcgaa
     6781 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     6841 cactctcggc atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg
     6901 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     6961 ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct
     7021 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     7081 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     7141 caagtccgcc atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg
     7201 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     7261 gcttaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     7321 ccttcctgac caactgactg aagagcagat cgcagaattt aaagagcttt tctccctatt
     7381 tgacaaggac ggggatggga caataacaac caaggagctg gggacggtga tgcggtctct
     7441 ggggcagaac cccacagaag cagagctgca ggacatgatc aatgaagtag atgccgacgg
     7501 tgacggcaca atcgacttcc ctgagttcct gacaatgatg gcaagaaaaa tgaaatacag
     7561 ggacacggaa gaagaaatta gagaagcgtt cggtgtgttt gataaggatg gcaatggcta
     7621 catcagtgca gcagagcttc gccacgtgat gacaaacctt ggagagaagt taacagatga
     7681 agaggttgat gaaatgatca gggaagcaga catcgatggg gatggtcagg taaactacga
     7741 agagtttgta caaatgatga cagcgaagtg aacgcgttac gtaaagcttg tcgataatca
     7801 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7861 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7921 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7981 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     8041 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8101 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8161 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta
     8221 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8281 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8341 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgac accgagcgct
     8401 gctcgaggcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     8461 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     8521 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     8581 caagggggag gattgggaag acaatagcag gcatgctggg gacacgtggg atcctctaga
     8641 gccccatcct cactgactcc gtcctggagt tggatgagag ataatggcct tacgttgtgc
     8701 caggggaggg tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt
     8761 ggcacagctg cccacggagg tgggggggtc accgtccctg gaggtgatga agaactgtgg
     8821 ggatgtggca ctgagggaca tggccagtgg gcacggtggg tgggttgggg ttggtcttgg
     8881 ggatcttgga gggcttttcc agccttcatg atttgacgat tgtatgaaca tctacatggc
     8941 aattctccag ctgcctgtcc cagtcctact gacccagctg tatctctcca ggcaagctct
     9001 tccacccctt ctgcttgcat ccagacacca tcaaacatgc aggctcagac acagggacca
     9061 gcagtgtctg tggccttttt gtgctcctct ccatgctggg ttttaacttg ctctttgtcc
     9121 ttctatccta tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc
     9181 agagccctca acacctgcat gtcccacgtc catgctgtcc tgcacttcct tatccccaag
     9241 atctgcctct ccgtgatgca ctgaattggc aaacatgtgt caccccagac caacaatgtc
     9301 acagcaaact cccccttgat aggacaaggg ggaatggctt tacactgaga caggggaggt
     9361 ttgggttgga tatgaggagg cagtttttcc cccagagggt ggtgacgcac tgaacaggtt
     9421 gcccaaggag gctgtggatg ccccatccct gcaggcattc aaggccaggc tggatgtggc
     9481 tctgggcagc ctgggctgct ggttgatgac cctgcacata gcagggggtt ggatctggat
     9541 gagcactgtg ctcctttgca acccaggccg ttctatgatt ctgtcattct aaatctctct
     9601 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     9661 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     9721 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     9781 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     9841 tccctctaga gccccatcct cactgactcc gtcctggagt tggatgagag ataatggcct
     9901 tacgttgtgc caggggaggg tcgggctgga tttagcaaga tttaccttct ccaaagagcg
     9961 gtgctgcagt ggcacagctg cccacggagg tgggggggtc accgtccctg gaggtgatga
    10021 agaactgtgg ggatgtggca ctgagggaca tggccagtgg gcacggtggg tgggttgggg
    10081 ttggtcttgg ggatcttgga gggcttttcc agccttcatg atttgacgat tgtatgaaca
    10141 tctacatggc aattctccag ctgcctgtcc cagtcctact gacccagctg tatctctcca
    10201 ggcaagctct tccacccctt ctgcttgcat ccagacacca tcaaacatgc aggctcagac
    10261 acagggacca gcagtgtctg tggccttttt gtgctcctct ccatgctggg ttttaacttg
    10321 ctctttgtcc ttctatccta tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg
    10381 agagtgtccc agagccctca acacctgcat gtcccacgtc catgctgtcc tgcacttcct
    10441 tatccccaag atctgcctct ccgtgatgca ctgaattggc aaacatgtgt caccccagac
    10501 caacaatgtc acagcaaact cccccttgat aggacaaggg ggaatggctt tacactgaga
    10561 caggggaggt ttgggttgga tatgaggagg cagtttttcc cccagagggt ggtgacgcac
    10621 tgaacaggtt gcccaaggag gctgtggatg ccccatccct gcaggcattc aaggccaggc
    10681 tggatgtggc tctgggcagc ctgggctgct ggttgatgac cctgcacata gcagggggtt
    10741 ggatctggat gagcactgtg ctcctttgca acccaggccg ttctatgatt ctgtcattct
    10801 aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
    10861 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
    10921 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
    10981 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
    11041 gggggggctg tccctctaga gtcgacggcg cgccctgcag gaatctagtt attaatagta
    11101 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
    11161 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    11221 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    11281 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
    11341 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
    11401 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
    11461 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
    11521 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc
    11581 aggsggggcg gggsggggsg rggggsgggg cggggcgagg cggagaggtg cggcggcagc
    11641 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
    11701 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
    11761 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
    11821 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
    11881 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
    11941 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
    12001 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
    12061 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
    12121 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
    12181 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
    12241 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
    12301 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
    12361 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
    12421 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
    12481 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
    12541 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
    12601 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
    12661 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
    12721 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
    12781 tcattttggc aaagaattga tttgataccg cgggccccgc gtggacggta ccgaagttcc
    12841 tattctctag aaagtatagg aacttccctc agcaccatgg ctagcggcag cctcggagtt
    12901 tgaatagata gaataaaata tctttatttt cattccatct gtgtgttggt tttttgtgtg
    12961 agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
    13021 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
    13081 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
    13141 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
    13201 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcaggctc
    13261 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
    13321 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
    13381 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
    13441 tccttctgcc tcagctaatt gagtaggggg gaggctaact gaaacacgga aggagacaat
    13501 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
    13561 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
    13621 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
    13681 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccgt ggaagttcct
    13741 attctctaga aagtatagga acttcacgcg cgcgtccatt aattccgccc ctctccctcc
    13801 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
    13861 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
    13921 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
    13981 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
    14041 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
    14101 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
    14161 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
    14221 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
    14281 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
    14341 ctttgaaaaa cacgatgata ataattcacc atgtctagac tggacaagag caaagtcata
    14401 aactctgctc tggaattact caatgaagtc ggtatcgaag gcctgacgac aaggaaactc
    14461 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
    14521 ctgctcgatg ccctggcaat cgagatgctg gacaggcatc atacccactt ctgccccctg
    14581 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcattccg ctgtgctctc
    14641 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
    14701 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
    14761 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggatcaggag
    14821 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
    14881 agacaagcaa ttgagctgtt cgaccatcag ggagccgaac ctgccttcct tttcggcctg
    14941 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac
    15001 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
    15061 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta
    15121 agtaaggatc aaatggcgcg ttacgtaaag cttgtcgata atcaacctct ggattacaaa
    15181 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    15241 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    15301 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    15361 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    15421 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    15481 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    15541 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt
    15601 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    15661 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    15721 cggatctccc tttgggccgc ctccccgcat cgacaccgag cgctgctcga ggcctcgact
    15781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    15841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    15901 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    15961 gaagacaata gcaggcatgc tggggacacg tgggatccgg tgccagggcg tgcccttggg
    16021 ctccccgggc gcgtttgggc ggccgcatcg aattctaccg ggtaggggag gcgcttttcc
    16081 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
    16141 gcctctggcc tcgcacacat tccacatcct ccggtaggcg ccaaccggct ccgttctttg
    16201 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
    16261 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
    16321 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
    16381 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
    16441 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
    16501 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
    16561 cctgcaatcg ccgctagccg ccaccatgaa aaagcctgaa ctcaccgcga cgtctgtcga
    16621 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
    16681 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
    16741 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
    16801 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
    16861 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
    16921 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
    16981 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
    17041 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgt
    17101 aagtgctagc ccctaggcct gaatcgaaaa accgctcagc ggcgcccgca cccaaacgaa
    17161 aacacccccg gcccaagcgg accgggggtg tcgcgtgaaa tggatcagtg agcgtggccg
    17221 tggtggtggc cgtggccgtg ctcgtcctcg tcggccggct tgtcgacgac ggcggtctcc
    17281 gtcgtcagga tcatccgggc caccgaggcg gcgttgagaa cagccgagcg ggtcaccttg
    17341 gccggatcga cgacgccggc ggagaccaga tcaccgaact ccagcgtggc ggcgttgaag
    17401 ccctgcccct tgggaagttc ggagagagct cgtcgactgc agaggcctgc atgcaagctt
    17461 ggcgtaatca tggtcatagc tgtttcctta tcagctcact caaaggcggt aatacggtta
    17521 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    17581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    17641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    17701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    17761 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    17821 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    17881 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    17941 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    18001 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    18061 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    18121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    18181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    18241 tggaa
//
