LOCUS       sequence-356655-        9748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into 
            pLX_HA backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Pan J, Kwon JJ, Talamas JA, Borah AA, Vazquez F, Boehm JS, Tsherniak
            A, Zitnik M, McFarland JM, Hahn WC
  TITLE     Sparse dictionary learning recovers pleiotropy from human cell 
            fitness screens.
  JOURNAL   Cell Syst. 2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 
            10.1016/j.cels.2021.12.005.
  PUBMED    35085500
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 
            10.1016/j.cels.2021.12.005."
COMMENT     Sequence Label: pLX_HA-C7orf26v1
FEATURES             Location/Qualifiers
     source          1..9748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      5..140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(160..179)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(161..179)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(189..206)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(189..205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(198..220)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      347..802
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(434..453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     644..665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        828..932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(933..1001,1002..1793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1151..1170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1964..2552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2453..2472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2706..2723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2840..2861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2876..2881,2882..2899,2900..2906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2914..2930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2919..2941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2938..2954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2938..2954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2973..2993
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2975..2993
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3021..3247
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3248..3428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3475..3600
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4093..4326
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4511..4555
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4786..4996
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4808..5004
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4855..4990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5036..5638
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(5036..5055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5532..5552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5681..5798
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5936..7114
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6167..7105
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7062..7082
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7134..7158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8512..8536)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8544..8570
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8621..9209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8674..8694)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9092..9103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9281..9514
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9592..9713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9629..9648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9683..9702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
       61 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      121 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
      181 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      241 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      301 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
      361 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      421 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      481 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      541 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      601 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      661 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      721 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
      781 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      841 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      901 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      961 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1021 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1081 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1141 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1201 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1261 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1321 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1381 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1441 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1501 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1561 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1621 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1681 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1741 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1801 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1861 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1921 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1981 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2041 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2101 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2161 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2221 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2281 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2341 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2401 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2461 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2521 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2581 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2641 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2701 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2761 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2821 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2881 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2941 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     3001 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     3061 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     3121 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     3181 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     3241 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3301 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3361 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3421 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     3481 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     3541 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     3601 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3661 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3721 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3781 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3841 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3901 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3961 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     4021 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     4081 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     4141 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     4201 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     4261 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     4321 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     4381 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     4441 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     4501 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     4561 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     4621 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     4681 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     4741 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4801 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4861 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4921 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4981 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     5041 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     5101 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     5161 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     5221 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     5281 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     5341 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     5401 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     5461 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     5521 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     5581 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
     5641 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     5701 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5761 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     5821 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
     5881 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
     5941 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     6001 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     6061 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     6121 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     6181 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     6241 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     6301 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     6361 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     6421 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     6481 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     6541 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     6601 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     6661 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     6721 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     6781 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     6841 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     6901 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     6961 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     7021 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     7081 tcaaagtttt tttcttccat ttcaggtgtc gtgaggctag catcgattga tcaacaagtt
     7141 tgtacaaaaa agcaggctta accatgagcg acatccgcca ctcgctgctg cgccgcgatg
     7201 cgctgagcgc cgccaaggag gtgttgtacc acctggacat ctacttcagc agccagctgc
     7261 agagcgcgcc gctgcccatc gtggacaagg gccccgtgga gctgctggag gagttcgtgt
     7321 tccaggtgcc caaggagcgc agcgcgcagc ccaagagact gaattccctt caggagcttc
     7381 aacttcttga aatcatgtgc aattatttcc aggagcaaac caaggactct gttcggcaga
     7441 ttattttttc atcccttttc agccctcaag ggaacaaagc cgatgacagc cggatgagct
     7501 tgttgggaaa actggtctcc atggcggtgg ctgtgtgtcg aatcccggtg ttggagtgtg
     7561 ctgcctcctg gcttcagcgg acgcccgtgg tttactgtgt gaggttagcc aaggcccttg
     7621 tagatgacta ctgctgtttg gtgccgggat ccattcagac gctgaagcag atattcagtg
     7681 ccagcccgag attctgctgc cagttcatca cctccgttac cgcgctctat gacctgtcat
     7741 cagatgacct cattccacct atggacttgc ttgaaatgat tgtcacctgg atttttgagg
     7801 acccaaggtt gattctcatc acttttttaa atactccgat tgcggccaat ctgccaatag
     7861 gattcttaga gctcaccccg ctcgttggat tgatccgctg gtgcgtgaag gcacccctgg
     7921 cttataaaag gaaaaagaag ccccccttat ccaatggcca tgtcagcaac aaggtcacaa
     7981 aggacccggg cgtggggatg gacagagact cccacctctt gtactcaaaa ctccacctca
     8041 gcgtcctgca agtgctcatg acgctgcagc tgcacctgac cgagaagaat ctgtatgggc
     8101 gcctggggct gatcctcttc gaccacatgg tcccgctggt agaggagatc aacaggttgg
     8161 cggatgaact gaaccccctc aacgcctccc aggagattga gctctcgctg gaccggctgg
     8221 cgcaggctct gcaggtggcc atggcctcag gagctctgct gtgcacgaga gatgacctga
     8281 gaaccttgtg ctccaggctg ccccataata acctcctcca gctggtgatc tcgggtcccg
     8341 tgcagcagtc gcctcacgcc gcgctccccc cggggttcta cccccacatc cacacgcccc
     8401 cgctgggcta cggggctgtc ccggcccacc ccgccgccca ccccgccctg cccacgcacc
     8461 ccggccacac cttcatctcc ggcgtgacct ttcccttcag gcccatccgc tacccagctt
     8521 tcttgtacaa agtggttgat atctacccat acgatgttcc agattacgct tagtaatgaa
     8581 ctagtaccgg ttaagtcgac aatcaacgcg ttaagtcgac aatcaacctc tggattacaa
     8641 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     8701 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     8761 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     8821 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     8881 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     8941 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     9001 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     9061 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     9121 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     9181 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     9241 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9301 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9361 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9421 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9481 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     9541 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     9601 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9661 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9721 tggctctagc tatcccgccc ctaactcc
//
