LOCUS       sequence-356675-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into 
            pLX_317 backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Pan J, Kwon JJ, Talamas JA, Borah AA, Vazquez F, Boehm JS, Tsherniak
            A, Zitnik M, McFarland JM, Hahn WC
  TITLE     Sparse dictionary learning recovers pleiotropy from human cell 
            fitness screens.
  JOURNAL   Cell Syst. 2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 
            10.1016/j.cels.2021.12.005.
  PUBMED    35085500
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 
            10.1016/j.cels.2021.12.005."
COMMENT     Sequence Label: pLX_317-C7orf26v1
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..1361
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          414..1352
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1309..1329
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1381..1405
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2759..2783)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2791..2832
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2883..3471
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2936..2956)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3354..3365)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3543..3776
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3854..3975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3891..3910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3945..3964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4015..4150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4170..4189)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4171..4189)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4199..4216)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4199..4215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4208..4230)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4357..4812
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4444..4463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4654..4675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4838..4942
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4943..5011,5012..5803)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5161..5180)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5974..6562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6463..6482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6716..6733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6850..6871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6886..6891,6892..6909,6910..6916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6924..6940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6929..6951
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6948..6964
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6948..6964
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6983..7003
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6985..7003
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7031..7257
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7258..7438
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7485..7610
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8103..8336
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8521..8565
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8796..9006
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8818..9014
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8865..9000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9046..9648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9046..9065)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9542..9562
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(9691..9763,1..45)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
       61 gacagcagag atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt
      121 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      181 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      241 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      301 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      361 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      421 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      481 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      541 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      601 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      661 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      721 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
      781 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
      841 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
      901 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
      961 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     1021 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1081 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1141 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1201 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1261 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1321 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggctagcat cgattgatca
     1381 acaagtttgt acaaaaaagc aggcttaacc atgagcgaca tccgccactc gctgctgcgc
     1441 cgcgatgcgc tgagcgccgc caaggaggtg ttgtaccacc tggacatcta cttcagcagc
     1501 cagctgcaga gcgcgccgct gcccatcgtg gacaagggcc ccgtggagct gctggaggag
     1561 ttcgtgttcc aggtgcccaa ggagcgcagc gcgcagccca agagactgaa ttcccttcag
     1621 gagcttcaac ttcttgaaat catgtgcaat tatttccagg agcaaaccaa ggactctgtt
     1681 cggcagatta ttttttcatc ccttttcagc cctcaaggga acaaagccga tgacagccgg
     1741 atgagcttgt tgggaaaact ggtctccatg gcggtggctg tgtgtcgaat cccggtgttg
     1801 gagtgtgctg cctcctggct tcagcggacg cccgtggttt actgtgtgag gttagccaag
     1861 gcccttgtag atgactactg ctgtttggtg ccgggatcca ttcagacgct gaagcagata
     1921 ttcagtgcca gcccgagatt ctgctgccag ttcatcacct ccgttaccgc gctctatgac
     1981 ctgtcatcag atgacctcat tccacctatg gacttgcttg aaatgattgt cacctggatt
     2041 tttgaggacc caaggttgat tctcatcact tttttaaata ctccgattgc ggccaatctg
     2101 ccaataggat tcttagagct caccccgctc gttggattga tccgctggtg cgtgaaggca
     2161 cccctggctt ataaaaggaa aaagaagccc cccttatcca atggccatgt cagcaacaag
     2221 gtcacaaagg acccgggcgt ggggatggac agagactccc acctcttgta ctcaaaactc
     2281 cacctcagcg tcctgcaagt gctcatgacg ctgcagctgc acctgaccga gaagaatctg
     2341 tatgggcgcc tggggctgat cctcttcgac cacatggtcc cgctggtaga ggagatcaac
     2401 aggttggcgg atgaactgaa ccccctcaac gcctcccagg agattgagct ctcgctggac
     2461 cggctggcgc aggctctgca ggtggccatg gcctcaggag ctctgctgtg cacgagagat
     2521 gacctgagaa ccttgtgctc caggctgccc cataataacc tcctccagct ggtgatctcg
     2581 ggtcccgtgc agcagtcgcc tcacgccgcg ctccccccgg ggttctaccc ccacatccac
     2641 acgcccccgc tgggctacgg ggctgtcccg gcccaccccg ccgcccaccc cgccctgccc
     2701 acgcaccccg gccacacctt catctccggc gtgacctttc ccttcaggcc catccgctac
     2761 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2821 ctcgattcta cgtagtaatg aactagtacc ggttaagtcg acaatcaacg cgttaagtcg
     2881 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2941 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3001 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3061 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3121 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3181 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3241 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3301 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3361 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3421 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     3481 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     3541 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     3601 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3661 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     3721 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     3781 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     3841 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3901 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3961 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     4021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4141 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     4201 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4261 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4321 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     4381 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4441 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4501 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4561 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4621 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4681 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4741 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4801 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4861 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4921 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4981 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5041 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5101 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5161 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5221 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5281 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5341 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5401 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5461 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5521 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5581 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5641 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5701 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5761 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5821 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5881 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5941 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6001 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6061 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6121 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6181 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6241 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6301 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6361 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6421 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6481 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6541 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6601 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6661 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6721 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6781 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6841 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6901 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     6961 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     7021 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     7081 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     7141 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     7201 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     7261 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7321 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7381 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7441 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     7501 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     7561 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7621 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     7681 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     7741 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     7801 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     7861 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     7921 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     7981 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8041 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8101 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8161 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8221 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8281 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8341 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8401 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     8461 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     8521 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8581 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     8641 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     8701 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt
     8761 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8821 gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8881 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8941 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9001 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag
     9061 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     9121 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     9181 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     9241 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     9301 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     9361 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     9421 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     9481 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     9541 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     9601 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat
     9661 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     9721 cagtgcaggg gaaagaatag tagacataat agcaacagac ata
//
