LOCUS       sequence-356677-       19436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19436)
  TITLE     Daigle Lab TIGRE3.0 plasmids 2022
REFERENCE   2  (bases 1 to 19436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1070-Ai211
            (TITL-ChrimsonR-tdT-Kv2.1-ICF-IR...
FEATURES             Location/Qualifiers
     source          1..19436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..79
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             98..901
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(161..180)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     771..790
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     930..952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     944..961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     945..961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1105..1154
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     primer_bind     complement(1320..1341)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1581..1600)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        4304..4618
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4312..4330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4348..4366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4383..4401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4419..4437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4455..4473
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4490..4508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4526..4544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4591..4615
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(4628..4661)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4738..4786
                     /note="synthetic polyadenylation signal"
     polyA_signal    4795..5271
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    5283..5744
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(5771..5804)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5820..5829
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5826..6875
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=ChrimsonR
                     /note="K176R mutant with accelerated closing kinetics"
                     /translation="MAELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTE
                     WWCDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSI
                     AIALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRY
                     FEWLLSCPVILIRLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIV
                     SCIYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLK
                     LSPYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEE
                     FVEEEDEDTV"
     misc_feature    8565..9153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9036..9047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9184..9391
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        11915..12218
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12220..12497
                     /label=chicken beta-actin promoter
     intron          12498..13514
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     13438..13460
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     13522..13541
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    13602..13635
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    13681..13729
                     /note="synthetic polyadenylation signal"
     polyA_signal    13738..14214
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(14249..14268)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14293..14341
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    14501..14534
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    14557..15130
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    14580..15132
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(14724..14741)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     14951..14970
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(15140..15757,15758..15763,15764..15799,15800..15802,
                     15803..15838,15839..15841,15842..15877,15878..15886)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    15927..16515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(16398..16409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    16546..16753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    16767..16800
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        16827..17326
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(16862..16885)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     17259..17278
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    18018..18067
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(18380..18968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18460..18479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgttttgg cggtttaaac attaagcacg tgttgacaat taatcatcgg catagtatat
       61 cggcatagta taatacgaca aggtgaggaa ctaaaccatg ggatcggcca ttgaacaaga
      121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
      181 acaacagacg atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
      301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
      361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
      421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
      481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
      541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
      601 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      721 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
      781 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
      841 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
      901 aagtgttaac tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      961 tgaattcgag ctcggtaccg aaaaaccgct cagcggcgcc cgcacccaaa cgaaaacacc
     1021 cccggcccaa gcggaccggg ggtgtcgcgt gaaatggatc agtgagcgtg gccgtggtgg
     1081 tggccgtggc cgtgctcgtc ctcgtcggcc ggcttgtcga cgacggcggt ctccgtcgtc
     1141 aggatcatcc gggccaccga ggcggcgttg agaacagccg agcgggtcac cttggccgga
     1201 tcgacgacgc cggcggagac cagatcaccg aactccagcg tggcggcgtt gaagccctgc
     1261 cccttgggaa gttcggagat ttcggccggc caatcgattt taccggggtg gtgcccatcc
     1321 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1381 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1441 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1501 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1561 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1621 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1681 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1741 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggtt
     1801 aattaagaat tcgagctcgc tagagcccca tcctcactga ctccgtcctg gagttggatg
     1861 agagataatg gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc
     1921 ttctccaaag agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc
     1981 cctggaggtg atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg
     2041 tgggtgggtt ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga
     2101 cgattgtatg aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca
     2161 gctgtatctc tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac
     2221 atgcaggctc agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc
     2281 tgggttttaa cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga
     2341 acgctgtgac ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct
     2401 gtcctgcact tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat
     2461 gtgtcacccc agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg
     2521 gctttacact gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga
     2581 gggtggtgac gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc
     2641 attcaaggcc aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca
     2701 catagcaggg ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat
     2761 gattctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt
     2821 gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc
     2881 cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc
     2941 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc
     3001 cctgggggct ttgggggggg gctgtccctc tagcgagctc ggtacccaga gccccatcct
     3061 cactgactcc gtcctggagt tggatgagag ataatggcct tacgttgtgc caggggaggg
     3121 tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt ggcacagctg
     3181 cccacggagg tgggggggtc accgtccctg gaggtgatga agaactgtgg ggatgtggca
     3241 ctgagggaca tggccagtgg gcacggtggg tgggttgggg ttggtcttgg ggatcttgga
     3301 gggcttttcc agccttcatg atttgacgat tgtatgaaca tctacatggc aattctccag
     3361 ctgcctgtcc cagtcctact gacccagctg tatctctcca ggcaagctct tccacccctt
     3421 ctgcttgcat ccagacacca tcaaacatgc aggctcagac acagggacca gcagtgtctg
     3481 tggccttttt gtgctcctct ccatgctggg ttttaacttg ctctttgtcc ttctatccta
     3541 tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc agagccctca
     3601 acacctgcat gtcccacgtc catgctgtcc tgcacttcct tatccccaag atctgcctct
     3661 ccgtgatgca ctgaattggc aaacatgtgt caccccagac caacaatgtc acagcaaact
     3721 cccccttgat aggacaaggg ggaatggctt tacactgaga caggggaggt ttgggttgga
     3781 tatgaggagg cagtttttcc cccagagggt ggtgacgcac tgaacaggtt gcccaaggag
     3841 gctgtggatg ccccatccct gcaggcattc aaggccaggc tggatgtggc tctgggcagc
     3901 ctgggctgct ggttgatgac cctgcacata gcagggggtt ggatctggat gagcactgtg
     3961 ctcctttgca acccaggccg ttctatgatt ctgtcattct aaatctctct ttcagcctaa
     4021 agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     4081 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
     4141 cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc
     4201 tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg tccctctagg
     4261 ggtacccggg gatccaccgg tatcgattac ctatcacaaa ttcgagttta ctccctatca
     4321 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt
     4381 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     4441 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt
     4501 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag
     4561 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgccta
     4621 tttaaatata acttcgtata gcatacatta tacgaagtta ttcgcgatga ataaatgaaa
     4681 gcttgcagat cccctcagca ccatggctag cggcagcctc ggagtttgaa tagatagaat
     4741 aaaatatctt tattttcatt ccatctgtgt gttggttttt tgtgtgagat ctacgggtgg
     4801 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     4861 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     4921 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     4981 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     5041 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc aggctcccga gttgttggga
     5101 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     5161 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     5221 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgcctcag
     5281 cgctgtaagt ctgcagaaat tgatgatcta ttaaacaata aagatgtcca ctaaaatgga
     5341 agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca ttttgaatgg
     5401 aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct gctctttact
     5461 gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat aatttaaaca
     5521 agcaaaacca aattaagggc cagctcattc ctcccactca tgatctatag atctatagat
     5581 ctctcgtggg atcattgttt ttctcttgat tcccactttg tggttctaag tactgtggtt
     5641 tccaaatgtg tcagtttcat agcctgaaga acgagatcag cagcctctgt tccacataca
     5701 cttcattctc agtattgttt tgccaagttc taattccatc agaaagcttt tacttaactg
     5761 aatagctaac ataacttcgt atagcataca ttatacgaag ttatcacgcg tgtttaaacg
     5821 ccaccatggc tgagctgatc agcagcgcca ccagatctct gtttgccgcc ggaggcatca
     5881 acccttggcc taacccctac caccacgagg acatgggctg tggaggaatg acacctacag
     5941 gcgagtgctt cagcaccgag tggtggtgtg acccttctta cggactgagc gacgccggat
     6001 acggatattg cttcgtggag gccacaggcg gctacctggt cgtgggagtg gagaagaagc
     6061 aggcttggct gcacagcaga ggcacaccag gagaaaagat cggcgcccag gtctgccagt
     6121 ggattgcttt cagcatcgcc atcgccctgc tgacattcta cggcttcagc gcctggaagg
     6181 ccacttgcgg ttgggaggag gtctacgtct gttgcgtcga ggtgctgttc gtgaccctgg
     6241 agatcttcaa ggagttcagc agccccgcca cagtgtacct gtctaccggc aaccacgcct
     6301 attgcctgcg ctacttcgag tggctgctgt cttgccccgt gatcctgatc agactgagca
     6361 acctgagcgg cctgaagaac gactacagca agcggaccat gggcctgatc gtgtcttgcg
     6421 tgggaatgat cgtgttcggc atggccgcag gactggctac cgattggctc aagtggctgc
     6481 tgtatatcgt gtcttgcatc tacggcggct acatgtactt ccaggccgcc aagtgctacg
     6541 tggaagccaa ccacagcgtg cctaaaggcc attgccgcat ggtcgtgaag ctgatggcct
     6601 acgcttactt cgcctcttgg ggcagctacc caatcctctg ggcagtggga ccagaaggac
     6661 tgctgaagct gagcccttac gccaacagca tcggccacag catctgcgac atcatcgcca
     6721 aggagttttg gaccttcctg gcccaccacc tgaggatcaa gatccacgag cacatcctga
     6781 tccacggcga catccggaag accaccaaga tggagatcgg aggcgaggag gtggaagtgg
     6841 aagagttcgt ggaggaggag gacgaggaca cagtggcggc accggtagta gcagtgagta
     6901 agggcgagga agtgatcaaa gagttcatgc ggtttaaggt gagaatggaa ggaagcatga
     6961 acggccacga gttcgaaatt gagggagaag gagagggacg gccctacgag ggcacccaga
     7021 cagccaagct gaaagtgaca aagggcgggc ctctgccatt cgcttgggac atcctgagcc
     7081 cacagtttat gtacggctcc aaggcctatg tgaaacatcc agctgacatt cccgattata
     7141 agaaactgag cttccccgag gggtttaagt gggaaagagt gatgaacttc gaggacggag
     7201 gcctggtgac tgtgacccag gacagctccc tgcaggatgg gaccctgatc tacaaggtga
     7261 aaatgagagg gacaaatttt ccccctgatg gacctgtgat gcagaagaaa actatgggat
     7321 gggaggcctc caccgaaagg ctgtatccac gcgacggggt gctgaaagga gaaatccacc
     7381 aggctctgaa gctgaaagat gggggacatt acctggtgga gttcaagaca atctacatgg
     7441 ccaagaaacc tgtgcagctg ccaggctact attacgtgga cacaaaactg gatatcactt
     7501 cacacaacga ggactacact attgtggagc agtatgaacg gagcgagggg agacaccatc
     7561 tgttcctggg ccatgggact ggaagtaccg gctcagggtc tagtggaacc gcctcaagcg
     7621 aggataacaa tatggctgtg atcaaagagt tcatgaggtt taaggtgcgc atggagggca
     7681 gcatgaatgg gcacgaattt gagattgaag gagagggcga agggaggcct tacgagggca
     7741 cacagactgc caagctgaaa gtgaccaagg gaggaccact gcctttcgct tgggatatcc
     7801 tgtctcctca gtttatgtac ggaagtaagg cctatgtcaa gcatcccgct gacattcctg
     7861 attacaagaa actgtctttc ccagagggct ttaagtggga gagagtgatg aattttgaag
     7921 atggaggcct ggtgaccgtg acacaggact cctctctgca ggatggcact ctgatctaca
     7981 aagtcaaaat gcgcggcacc aattttccac ccgatgggcc cgtgatgcag aagaaaacaa
     8041 tggggtggga ggccagcact gaacggctgt atcctagaga cggagtgctg aagggcgaaa
     8101 tccaccaggc cctgaagctg aaagacggcg gccactacct ggtggagttc aaaaccatct
     8161 acatggccaa gaaaccagtg cagctgcccg gctattacta tgtggacacc aagctggata
     8221 tcacatccca caatgaagac tacaccattg tggaacagta tgagaggtct gaaggacgcc
     8281 accatctgtt tctgtacggc atggatgagc tgtataagca gtcccagccc atcctcaaca
     8341 ccaaggagat ggccccgcag agcaagcctc cagaggagct ggagatgagc agcatgccca
     8401 gccccgtggc ccctctgccc gcacgcacgg agggcgtcat cgacatgcgg agcatgtcca
     8461 gcattgacag cttcatcagc tgtgccacgg acttccctga agccaccaga ttctaaggcc
     8521 tttttggcct gtacaagtga acgcgttacg taaagcttgt cgataatcaa cctctggatt
     8581 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8641 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8701 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8761 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8821 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8881 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8941 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct
     9001 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     9061 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     9121 cgagtcggat ctccctttgg gccgcctccc cgcatcgaca ccgagcgctg ctcgaggcct
     9181 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9241 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9301 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9361 attgggaaga caatagcagg catgctgggg acacgtggga tcctctagag ccccatcctc
     9421 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
     9481 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
     9541 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
     9601 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
     9661 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
     9721 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
     9781 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
     9841 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     9901 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     9961 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
    10021 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
    10081 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
    10141 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
    10201 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
    10261 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
    10321 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
    10381 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
    10441 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
    10501 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
    10561 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag
    10621 ccccatcctc actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc
    10681 aggggagggt cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg
    10741 gcacagctgc ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg
    10801 gatgtggcac tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg
    10861 gatcttggag ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca
    10921 attctccagc tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt
    10981 ccaccccttc tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag
    11041 cagtgtctgt ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct
    11101 tctatcctat cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca
    11161 gagccctcaa cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga
    11221 tctgcctctc cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca
    11281 cagcaaactc ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt
    11341 tgggttggat atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg
    11401 cccaaggagg ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct
    11461 ctgggcagcc tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg
    11521 agcactgtgc tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt
    11581 tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
    11641 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
    11701 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
    11761 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
    11821 ccctctagag tcgacggcgc gccctgcagg aatctagtta ttaatagtaa tcaattacgg
    11881 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
    11941 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
    12001 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
    12061 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
    12121 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
    12181 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
    12241 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
    12301 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgccr ggsggggsgg
    12361 ggsggggsgr ggggsggggs ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
    12421 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
    12481 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
    12541 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
    12601 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
    12661 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
    12721 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
    12781 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
    12841 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
    12901 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
    12961 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
    13021 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
    13081 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
    13141 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
    13201 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
    13261 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
    13321 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
    13381 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
    13441 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
    13501 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
    13561 aagaattgat ttgataccgc gggccccgcg tggacggtac cgaagttcct attctctaga
    13621 aagtatagga acttccctca gcaccatggc tagcggcagc ctcggagttt gaatagatag
    13681 aataaaatat ctttattttc attccatctg tgtgttggtt ttttgtgtga gatctacggg
    13741 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
    13801 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
    13861 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
    13921 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
    13981 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcaggctcc cgagttgttg
    14041 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
    14101 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
    14161 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgcct
    14221 cagctaattg agtagggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
    14281 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
    14341 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
    14401 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
    14461 ggcccagggc tcgcagccaa cgtcggggcg gcaggccgtg gaagttccta ttctctagaa
    14521 agtataggaa cttcacgcgc gcgtccatta attccgcccc tctccctccc ccccccctaa
    14581 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
    14641 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
    14701 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
    14761 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
    14821 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
    14881 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
    14941 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
    15001 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc
    15061 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
    15121 acgatgataa taattcacca tgtctagact ggacaagagc aaagtcataa actctgctct
    15181 ggaattactc aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
    15241 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
    15301 cctggcaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
    15361 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
    15421 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
    15481 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
    15541 gtccgccgtg ggccacttta cactgggctg cgtattggag gatcaggagc atcaagtagc
    15601 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
    15661 tgagctgttc gaccatcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
    15721 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg cccttgacga
    15781 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
    15841 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactaa accaggtaaa
    15901 tggcgcgtta cgtaaagctt gtcgataatc aacctctgga ttacaaaatt tgtgaaagat
    15961 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
    16021 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
    16081 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
    16141 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt caactccttt
    16201 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
    16261 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
    16321 aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt
    16381 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
    16441 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
    16501 gggccgcctc cccgcatcga caccgagcgc tgctcgaggc ctcgactgtg ccttctagtt
    16561 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
    16621 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
    16681 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
    16741 ggcatgctgg ggacacgtgg gatccggtgc cagggcgtgc ccttgggctc cccgggcgcg
    16801 tttgggcggc cgcatcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
    16861 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
    16921 cacacattcc acatcctccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
    16981 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
    17041 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
    17101 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
    17161 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
    17221 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
    17281 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcaatcgccg
    17341 ctagccgcca ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
    17401 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
    17461 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
    17521 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
    17581 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
    17641 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
    17701 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
    17761 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
    17821 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgtaag tgctagcccc
    17881 taggcctgaa tcgaaaaacc gctcagcggc gcccgcaccc aaacgaaaac acccccggcc
    17941 caagcggacc gggggtgtcg cgtgaaatgg atcagtgagc gtggccgtgg tggtggccgt
    18001 ggccgtgctc gtcctcgtcg gccggcttgt cgacgacggc ggtctccgtc gtcaggatca
    18061 tccgggccac cgaggcggcg ttgagaacag ccgagcgggt caccttggcc ggatcgacga
    18121 cgccggcgga gaccagatca ccgaactcca gcgtggcggc gttgaagccc tgccccttgg
    18181 gaagttcgga gagagctcgt cgactgcaga ggcctgcatg caagcttggc gtaatcatgg
    18241 tcatagctgt ttccttatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    18301 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    18361 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    18421 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    18481 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    18541 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    18601 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    18661 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    18721 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    18781 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    18841 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    18901 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    18961 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    19021 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    19081 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    19141 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    19201 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    19261 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    19321 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    19381 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgc
//
