LOCUS       sequence-356678-       10595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human PD-1 (Y223F, Y248F) fused to mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma
            Y, Wang H, Bennett EJ, Fu G, Hui E
  TITLE     PD-1 and BTLA regulate T cell signaling differentially and only 
            partially through SHP1 and SHP2.
  JOURNAL   J Cell Biol. 2020 Jun 1;219(6). pii: 151801. doi: 
            10.1083/jcb.201905085.
  PUBMED    32437509
REFERENCE   2  (bases 1 to 10595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-06-1"; volume: "219(6). pii: 151801. doi"; 
            pages: " 10.1083/jcb.201905085"
COMMENT     Sequence Label: pHR-PD-1 (Y223F, Y248F)-mGFP
FEATURES             Location/Qualifiers
     source          1..10595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     156..177
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    283..871
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(336..356)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(754..765)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(874..890)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(875..891)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             981..1214
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1239..1257)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1240..1257)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1547..1566)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1780..1799)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1899..1921
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1959..1977)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2045..2149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2150..2218,2219..3010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2368..2387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3181..3769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3670..3689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3923..3940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4010..4030)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4015..4344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4195..4330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5478..5543
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5673..5693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6118..6252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6155..6174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6209..6228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6420..7053
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7100..7225
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7722..7955
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8140..8184
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8469..8586
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8739..9146
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      10092..10101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10098..10100,10101..10103,10104..10595,1..222)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10143..10164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10404..10423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
       61 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
      121 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      181 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
      241 gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
      301 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
      361 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
      421 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
      481 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
      541 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
      601 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
      661 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
      721 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
      781 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
      841 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa
      901 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
      961 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     1021 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     1081 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     1141 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1201 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     1261 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     1321 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     1381 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     1441 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     1501 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     1561 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     1621 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     1681 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     1741 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     1801 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     1861 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     1921 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     1981 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     2041 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2101 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2161 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2221 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2281 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2341 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2401 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2461 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2521 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2581 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2641 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2701 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2761 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2821 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2881 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2941 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3001 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3061 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3121 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3181 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3301 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3421 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3541 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3841 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3901 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3961 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     4021 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4081 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4141 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4201 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4261 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4321 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     4381 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     4441 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     4501 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     4561 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     4621 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     4681 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     4741 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     4801 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     4861 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     4921 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     4981 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     5041 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     5101 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     5161 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     5221 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     5281 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     5341 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     5401 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     5461 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     5521 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     5581 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     5641 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     5701 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     5761 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     5821 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     5881 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     5941 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     6001 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     6061 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     6121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6241 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     6301 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     6361 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     6421 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     6481 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     6541 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     6601 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     6661 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     6721 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     6781 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     6841 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     6901 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6961 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7021 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     7081 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     7141 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     7201 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     7261 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     7321 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     7381 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     7441 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     7501 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     7561 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7621 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7681 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7741 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     7801 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7861 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7921 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7981 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     8041 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     8101 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     8161 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     8221 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     8281 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     8341 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8401 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     8461 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     8521 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8581 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     8641 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     8701 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     8761 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     8821 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     8881 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     8941 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     9001 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     9061 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     9121 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcatgcaga
     9181 tcccacaggc gccctggcca gtcgtctggg cggtgctaca actgggctgg cggccaggat
     9241 ggttcttaga ctccccagac aggccctgga acccccccac cttctcccca gccctgctcg
     9301 tggtgaccga aggggacaac gccaccttca cctgcagctt ctccaacaca tcggagagct
     9361 tcgtgctaaa ctggtaccgc atgagcccca gcaaccagac ggacaagctg gccgctttcc
     9421 ccgaggaccg cagccagccc ggccaggact gccgcttccg tgtcacacaa ctgcccaacg
     9481 ggcgtgactt ccacatgagc gtggtcaggg cccggcgcaa tgacagcggc acctacctct
     9541 gtggggccat ctccctggcc cccaaggcgc agatcaaaga gagcctgcgg gcagagctca
     9601 gggtgacaga gagaagggca gaagtgccca cagcccaccc cagcccctca cccaggccag
     9661 ccggccagtt ccaaaccctg gtggttggtg tcgtgggcgg cctgctgggc agcctggtgc
     9721 tgctagtctg ggtcctggcc gtcatctgct cccgggccgc acgagggaca ataggagcca
     9781 ggcgcaccgg ccagcccctg aaggaggacc cctcagccgt gcctgtgttc tctgtggact
     9841 ttggggagct ggatttccag tggcgagaga agaccccgga gccccccgtg ccctgtgtcc
     9901 ctgagcagac ggagtttgcc accattgtct ttcctagcgg aatgggcacc tcatcccccg
     9961 cccgcagggg ctcagccgac ggccctcgga gtgcccagcc actgaggcct gaggatggac
    10021 actgctcttg gcccctgacg cgtctgcagg atatcaagct tgcggtaccg cgggcccggg
    10081 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
    10141 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
    10201 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    10261 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
    10321 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
    10381 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
    10441 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
    10501 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
    10561 ccgacaagca gaagaacggc atcaaggtga acttc
//
