LOCUS       sequence-356679-       10598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human BTLA fused to mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma
            Y, Wang H, Bennett EJ, Fu G, Hui E
  TITLE     PD-1 and BTLA regulate T cell signaling differentially and only 
            partially through SHP1 and SHP2.
  JOURNAL   J Cell Biol. 2020 Jun 1;219(6). pii: 151801. doi: 
            10.1083/jcb.201905085.
  PUBMED    32437509
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-06-1"; volume: "219(6). pii: 151801. doi"; 
            pages: " 10.1083/jcb.201905085"
COMMENT     Sequence Label: pHR-BTLA-mGFP
FEATURES             Location/Qualifiers
     source          1..10598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..185
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    291..879
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(344..364)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(762..773)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(882..898)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(883..899)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             989..1222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1247..1265)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1248..1265)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1555..1574)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1788..1807)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1907..1929
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1967..1985)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2053..2157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2158..2226,2227..3018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2376..2395)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3189..3777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3678..3697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3931..3948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4018..4038)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4023..4352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4203..4338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5486..5551
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5681..5701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6126..6260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6163..6182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6217..6236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6428..7061
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7108..7233
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7730..7963
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8148..8192
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8477..8594
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8747..9154
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      10103..10112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10109..10111,10112..10114,10115..10598,1..230)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10154..10175)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10415..10434)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
       61 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
      121 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      181 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
      241 actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc
      301 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
      361 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
      421 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
      481 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
      541 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
      601 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
      661 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
      721 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
      781 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
      841 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
      901 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
      961 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     1021 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     1081 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     1141 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     1201 tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta tagtgtcacc
     1261 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     1321 atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta tcatctctct
     1381 cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct ggtaacgccg
     1441 tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc cagatcctct
     1501 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     1561 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     1621 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     1681 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     1741 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
     1801 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     1861 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     1921 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     1981 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2161 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2221 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2281 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2341 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2401 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2461 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2521 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2581 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2641 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2701 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2761 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2821 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2881 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2941 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3001 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3061 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3301 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3781 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3841 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3901 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3961 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga
     4021 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4081 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4141 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4201 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4261 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     4321 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag
     4381 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
     4441 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
     4501 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
     4561 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
     4621 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg
     4681 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
     4741 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
     4801 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
     4861 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
     4921 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
     4981 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
     5041 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
     5101 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     5161 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
     5221 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
     5281 attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt
     5341 accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag
     5401 tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     5461 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     5521 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     5581 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     5641 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     5701 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     5761 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     5821 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     5881 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     5941 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     6001 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     6061 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     6121 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6181 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6241 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     6301 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     6361 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     6421 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     6481 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     6541 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     6601 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     6661 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     6721 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     6781 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     6841 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     6901 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6961 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7021 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     7081 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     7141 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     7201 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     7261 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     7321 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     7381 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     7441 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     7501 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     7561 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     7621 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     7681 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     7741 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     7801 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     7861 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     7921 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     7981 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     8041 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     8101 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     8161 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     8221 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     8281 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     8341 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     8401 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccaaatgg
     8461 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     8521 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     8581 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg atcgataagc
     8641 ttgatatcga attcctgcag ccccgataaa ataaaagatt ttatttagtc tccagaaaaa
     8701 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gctgcagtaa cgccattttg
     8761 caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg cgggtacatg
     8821 aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg ccccggcccg
     8881 gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag aacagatggt
     8941 ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt tccaggctcc
     9001 cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc tgcttctcgc
     9061 ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc cctcactcgg
     9121 cgcgccagtc ctccgacaga ctgagtcgcc cgggggggat ctggagctct cgagaattct
     9181 catgaagaca ttgcctgcca tgcttggaac tgggaaatta ttttgggtct tcttcttaat
     9241 cccatatctg gacatctgga acatccatgg gaaagaatca tgtgatgtac agctttatat
     9301 aaagagacaa tctgaacact ccatcttagc aggagatccc tttgaactag aatgccctgt
     9361 gaaatactgt gctaacaggc ctcatgtgac ttggtgcaag ctcaatggaa caacatgtgt
     9421 aaaacttgaa gatagacaaa caagttggaa ggaagagaag aacatttcat ttttcattct
     9481 acattttgaa ccagtgcttc ctaatgacaa tgggtcatac cgctgttctg caaattttca
     9541 gtctaatctc attgaaagcc actcaacaac tctttatgtg acagatgtaa aaagtgcctc
     9601 agaacgaccc tccaaggacg aaatggcaag cagaccctgg ctcctgtatc gtttacttcc
     9661 tttgggggga ttgcctctac tcatcactac ctgtttctgc ctgttctgct gcctgagaag
     9721 gcaccaagga aagcaaaatg aactctctga cacagcagga agggaaatta acctggttga
     9781 tgctcacctt aagagtgagc aaacagaagc aagcaccagg caaaattccc aagtactgct
     9841 atcagaaact ggaatttatg ataatgaccc tgacctttgt ttcaggatgc aggaagggtc
     9901 tgaagtttat tctaatccat gcctggaaga aaacaaacca ggcattgttt atgcttccct
     9961 gaaccattct gtcattggac tgaactcaag actggcaaga aatgtaaaag aagcaccaac
    10021 agaatatgca tccatatgtg tgaggagtac gcgtctgcag gatatcaagc ttgcggtacc
    10081 gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
    10141 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
    10201 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
    10261 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
    10321 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
    10381 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
    10441 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
    10501 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
    10561 ctatatcatg gccgacaagc agaagaacgg catcaagg
//
