LOCUS       sequence-356680-       10598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to 
            mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma
            Y, Wang H, Bennett EJ, Fu G, Hui E
  TITLE     PD-1 and BTLA regulate T cell signaling differentially and only 
            partially through SHP1 and SHP2.
  JOURNAL   J Cell Biol. 2020 Jun 1;219(6). pii: 151801. doi: 
            10.1083/jcb.201905085.
  PUBMED    32437509
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-06-1"; volume: "219(6). pii: 151801. doi"; 
            pages: " 10.1083/jcb.201905085"
COMMENT     Sequence Label: pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFP
FEATURES             Location/Qualifiers
     source          1..10598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..22)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7..23)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             113..346
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(371..389)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(372..389)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(679..698)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(912..931)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1031..1053
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1091..1109)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1177..1281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1282..1350,1351..2142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1500..1519)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2313..2901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2802..2821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3055..3072
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3142..3162)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3147..3476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3327..3462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4610..4675
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4805..4825
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5250..5384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5287..5306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5341..5360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5552..6185
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6232..6357
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6854..7087
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7272..7316
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7601..7718
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7871..8278
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      9227..9236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9233..9235,9236..9238,9239..9952)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9278..9299)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9539..9558)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9886..9907
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(10013..10598,1..3)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10066..10086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10484..10495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg
       61 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
      121 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
      181 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      241 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      301 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
      361 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
      421 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
      481 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
      541 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
      601 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
      661 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
      721 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
      781 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
      841 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
      901 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
      961 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1021 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1081 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1141 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1201 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1261 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1321 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1381 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1441 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1501 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1561 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1621 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1681 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1741 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1801 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     1861 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1921 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1981 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2041 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2101 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2701 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3001 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3061 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3121 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3181 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3241 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3301 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3361 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3421 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     3481 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     3541 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     3601 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     3661 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     3721 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     3781 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3841 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3901 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     3961 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     4021 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
     4081 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     4141 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
     4201 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     4261 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     4321 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
     4381 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
     4441 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
     4501 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
     4561 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     4621 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     4681 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     4741 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     4801 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     4861 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     4921 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     4981 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     5041 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     5101 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     5161 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5221 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5281 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5341 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     5401 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     5461 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     5521 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
     5581 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     5641 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     5701 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     5761 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     5821 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     5881 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     5941 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     6001 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6061 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6121 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6181 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     6241 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     6301 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     6361 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     6421 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     6481 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     6541 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     6601 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     6661 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     6721 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     6781 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     6841 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     6901 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     6961 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     7021 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     7081 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     7141 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     7201 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     7261 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     7321 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     7381 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     7441 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     7501 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     7561 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     7621 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     7681 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     7741 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     7801 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     7861 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     7921 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     7981 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     8041 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     8101 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     8161 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     8221 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     8281 ggatctggag ctctcgagaa ttctcatgaa gacattgcct gccatgcttg gaactgggaa
     8341 attattttgg gtcttcttct taatcccata tctggacatc tggaacatcc atgggaaaga
     8401 atcatgtgat gtacagcttt atataaagag acaatctgaa cactccatct tagcaggaga
     8461 tccctttgaa ctagaatgcc ctgtgaaata ctgtgctaac aggcctcatg tgacttggtg
     8521 caagctcaat ggaacaacat gtgtaaaact tgaagataga caaacaagtt ggaaggaaga
     8581 gaagaacatt tcatttttca ttctacattt tgaaccagtg cttcctaatg acaatgggtc
     8641 ataccgctgt tctgcaaatt ttcagtctaa tctcattgaa agccactcaa caactcttta
     8701 tgtgacagat gtaaaaagtg cctcagaacg accctccaag gacgaaatgg caagcagacc
     8761 ctggctcctg tatcgtttac ttcctttggg gggattgcct ctactcatca ctacctgttt
     8821 ctgcctgttc tgctgcctga gaaggcacca aggaaagcaa aatgaactct ctgacacagc
     8881 aggaagggaa attaacctgg ttgatgctca ccttaagagt gagcaaacag aagcaagcac
     8941 caggcaaaat tcccaagtac tgctatcaga aactggaatt tttgataatg accctgacct
     9001 ttgtttcagg atgcaggaag ggtctgaagt tttttctaat ccatgcctgg aagaaaacaa
     9061 accaggcatt gtttttgctt ccctgaacca ttctgtcatt ggactgaact caagactggc
     9121 aagaaatgta aaagaagcac caacagaatt tgcatccata tgtgtgagga gtacgcgtct
     9181 gcaggatatc aagcttgcgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
     9241 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     9301 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     9361 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     9421 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     9481 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     9541 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     9601 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     9661 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     9721 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     9781 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     9841 cacccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     9901 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     9961 cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
    10021 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
    10081 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    10141 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    10201 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    10261 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    10321 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    10381 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    10441 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    10501 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    10561 ccctcagacg agtcggatct ccctttgggc cgcctccc
//
