LOCUS       sequence-356681-       17036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17036)
  TITLE     Daigle Lab TIGRE3.0 plasmids 2022
REFERENCE   2  (bases 1 to 17036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1181-Ai224 (TICL-nls-EGFP-ICF-nls-dTomato)
FEATURES             Location/Qualifiers
     source          1..17036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..79
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             98..901
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(161..180)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     771..790
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     930..952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     944..961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     945..961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1105..1154
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     enhancer        4377..4680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4682..4959
                     /label=chicken beta-actin promoter
     intron          4960..5976
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     protein_bind    complement(6070..6103)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6181..6229
                     /note="synthetic polyadenylation signal"
     polyA_signal    6238..6714
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    6726..7187
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(7214..7247)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7270..7290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7291..7293,7294..8007)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7291..7293,7294..8004)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     7941..7962
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8021..8228
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        10752..11055
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11057..11334
                     /label=chicken beta-actin promoter
     protein_bind    12440..12473
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    12519..12567
                     /note="synthetic polyadenylation signal"
     polyA_signal    12576..13052
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(13087..13106)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13131..13179
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    13339..13372
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             13409..13429
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13430..14128
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(13560..13578)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     13828..13847
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     14022..14045
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    14146..14353
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    14367..14400
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        14427..14926
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(14462..14485)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     14859..14878
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    15618..15667
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(15980..16568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16060..16079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgttttgg cggtttaaac attaagcacg tgttgacaat taatcatcgg catagtatat
       61 cggcatagta taatacgaca aggtgaggaa ctaaaccatg ggatcggcca ttgaacaaga
      121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
      181 acaacagacg atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
      301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
      361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
      421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
      481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
      541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
      601 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      721 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
      781 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
      841 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
      901 aagtgttaac tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      961 tgaattcgag ctcggtaccg aaaaaccgct cagcggcgcc cgcacccaaa cgaaaacacc
     1021 cccggcccaa gcggaccggg ggtgtcgcgt gaaatggatc agtgagcgtg gccgtggtgg
     1081 tggccgtggc cgtgctcgtc ctcgtcggcc ggcttgtcga cgacggcggt ctccgtcgtc
     1141 aggatcatcc gggccaccga ggcggcgttg agaacagccg agcgggtcac cttggccgga
     1201 tcgacgacgc cggcggagac cagatcaccg aactccagcg tggcggcgtt gaagccctgc
     1261 cccttgggaa gttcggagat ttcggccggc caatcgattt taccggggtg gtgcccatcc
     1321 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1381 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1441 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1501 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1561 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1621 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1681 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1741 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggtt
     1801 aattaagaat tcgagctcgc tagagcccca tcctcactga ctccgtcctg gagttggatg
     1861 agagataatg gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc
     1921 ttctccaaag agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc
     1981 cctggaggtg atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg
     2041 tgggtgggtt ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga
     2101 cgattgtatg aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca
     2161 gctgtatctc tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac
     2221 atgcaggctc agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc
     2281 tgggttttaa cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga
     2341 acgctgtgac ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct
     2401 gtcctgcact tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat
     2461 gtgtcacccc agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg
     2521 gctttacact gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga
     2581 gggtggtgac gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc
     2641 attcaaggcc aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca
     2701 catagcaggg ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat
     2761 gattctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt
     2821 gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc
     2881 cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc
     2941 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc
     3001 cctgggggct ttgggggggg gctgtccctc tagcgagctc ggtacccaga gccccatcct
     3061 cactgactcc gtcctggagt tggatgagag ataatggcct tacgttgtgc caggggaggg
     3121 tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt ggcacagctg
     3181 cccacggagg tgggggggtc accgtccctg gaggtgatga agaactgtgg ggatgtggca
     3241 ctgagggaca tggccagtgg gcacggtggg tgggttgggg ttggtcttgg ggatcttgga
     3301 gggcttttcc agccttcatg atttgacgat tgtatgaaca tctacatggc aattctccag
     3361 ctgcctgtcc cagtcctact gacccagctg tatctctcca ggcaagctct tccacccctt
     3421 ctgcttgcat ccagacacca tcaaacatgc aggctcagac acagggacca gcagtgtctg
     3481 tggccttttt gtgctcctct ccatgctggg ttttaacttg ctctttgtcc ttctatccta
     3541 tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc agagccctca
     3601 acacctgcat gtcccacgtc catgctgtcc tgcacttcct tatccccaag atctgcctct
     3661 ccgtgatgca ctgaattggc aaacatgtgt caccccagac caacaatgtc acagcaaact
     3721 cccccttgat aggacaaggg ggaatggctt tacactgaga caggggaggt ttgggttgga
     3781 tatgaggagg cagtttttcc cccagagggt ggtgacgcac tgaacaggtt gcccaaggag
     3841 gctgtggatg ccccatccct gcaggcattc aaggccaggc tggatgtggc tctgggcagc
     3901 ctgggctgct ggttgatgac cctgcacata gcagggggtt ggatctggat gagcactgtg
     3961 ctcctttgca acccaggccg ttctatgatt ctgtcattct aaatctctct ttcagcctaa
     4021 agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     4081 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
     4141 cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc
     4201 tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg tccctctagg
     4261 ggtacccggg gatccaccgg tccttaatta accgtttaaa caattctgca ggaatctagt
     4321 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4381 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4441 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4501 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4561 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4621 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4681 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     4741 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     4801 gggcgcgcgc crgggggggs gggggggggs ggggggsggg gsggggsgrg gcggagaggt
     4861 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     4921 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     4981 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     5041 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     5101 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     5161 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     5221 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     5281 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     5341 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     5401 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     5461 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     5521 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     5581 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     5641 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     5701 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     5761 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     5821 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     5881 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     5941 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     6001 gtgctgtctc atcattttgg caaagaattg atttgatacc atttaaatgg ggatcaaatt
     6061 caattaaata taacttcgta tagcatacat tatacgaagt tattcgcgag tgaataaatg
     6121 aaagcttgca gatcccctca gcaccatggc tagcggcagc ctcggagttt gaatagatag
     6181 aataaaatat ctttattttc attccatctg tgtgttggtt ttttgtgtga gatctacggg
     6241 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     6301 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     6361 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     6421 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     6481 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcaggctcc cgagttgttg
     6541 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     6601 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     6661 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgcct
     6721 cagcgctgta agtctgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat
     6781 ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa
     6841 tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt
     6901 actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa
     6961 acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata
     7021 gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg
     7081 gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat
     7141 acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaaagc ttttacttaa
     7201 ctgaatagct aacataactt cgtatagcat acattatacg aagttatcac gcgtgccgcc
     7261 accatgccac ccaaaaagaa aagaaaggtg gtgagcaagg gcgaggagct gttcaccggg
     7321 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     7381 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     7441 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     7501 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     7561 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     7621 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     7681 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     7741 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     7801 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     7861 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     7921 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     7981 ctcggcatgg atgagctgta caagtaactc gaggcctcga ctgtgccttc tagttgccag
     8041 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     8101 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8161 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     8221 gctggggaca cgtgggatcc tctagagccc catcctcact gactccgtcc tggagttgga
     8281 tgagagataa tggccttacg ttgtgccagg ggagggtcgg gctggattta gcaagattta
     8341 ccttctccaa agagcggtgc tgcagtggca cagctgccca cggaggtggg ggggtcaccg
     8401 tccctggagg tgatgaagaa ctgtggggat gtggcactga gggacatggc cagtgggcac
     8461 ggtgggtggg ttggggttgg tcttggggat cttggagggc ttttccagcc ttcatgattt
     8521 gacgattgta tgaacatcta catggcaatt ctccagctgc ctgtcccagt cctactgacc
     8581 cagctgtatc tctccaggca agctcttcca ccccttctgc ttgcatccag acaccatcaa
     8641 acatgcaggc tcagacacag ggaccagcag tgtctgtggc ctttttgtgc tcctctccat
     8701 gctgggtttt aacttgctct ttgtccttct atcctatctt cttatcctta aggctgttct
     8761 gaacgctgtg acttggagag tgtcccagag ccctcaacac ctgcatgtcc cacgtccatg
     8821 ctgtcctgca cttccttatc cccaagatct gcctctccgt gatgcactga attggcaaac
     8881 atgtgtcacc ccagaccaac aatgtcacag caaactcccc cttgatagga caagggggaa
     8941 tggctttaca ctgagacagg ggaggtttgg gttggatatg aggaggcagt ttttccccca
     9001 gagggtggtg acgcactgaa caggttgccc aaggaggctg tggatgcccc atccctgcag
     9061 gcattcaagg ccaggctgga tgtggctctg ggcagcctgg gctgctggtt gatgaccctg
     9121 cacatagcag ggggttggat ctggatgagc actgtgctcc tttgcaaccc aggccgttct
     9181 atgattctgt cattctaaat ctctctttca gcctaaagct ttttccccgt atccccccag
     9241 gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct
     9301 tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga
     9361 ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca
     9421 tccctggggg ctttgggggg gggctgtccc tctagagccc catcctcact gactccgtcc
     9481 tggagttgga tgagagataa tggccttacg ttgtgccagg ggagggtcgg gctggattta
     9541 gcaagattta ccttctccaa agagcggtgc tgcagtggca cagctgccca cggaggtggg
     9601 ggggtcaccg tccctggagg tgatgaagaa ctgtggggat gtggcactga gggacatggc
     9661 cagtgggcac ggtgggtggg ttggggttgg tcttggggat cttggagggc ttttccagcc
     9721 ttcatgattt gacgattgta tgaacatcta catggcaatt ctccagctgc ctgtcccagt
     9781 cctactgacc cagctgtatc tctccaggca agctcttcca ccccttctgc ttgcatccag
     9841 acaccatcaa acatgcaggc tcagacacag ggaccagcag tgtctgtggc ctttttgtgc
     9901 tcctctccat gctgggtttt aacttgctct ttgtccttct atcctatctt cttatcctta
     9961 aggctgttct gaacgctgtg acttggagag tgtcccagag ccctcaacac ctgcatgtcc
    10021 cacgtccatg ctgtcctgca cttccttatc cccaagatct gcctctccgt gatgcactga
    10081 attggcaaac atgtgtcacc ccagaccaac aatgtcacag caaactcccc cttgatagga
    10141 caagggggaa tggctttaca ctgagacagg ggaggtttgg gttggatatg aggaggcagt
    10201 ttttccccca gagggtggtg acgcactgaa caggttgccc aaggaggctg tggatgcccc
    10261 atccctgcag gcattcaagg ccaggctgga tgtggctctg ggcagcctgg gctgctggtt
    10321 gatgaccctg cacatagcag ggggttggat ctggatgagc actgtgctcc tttgcaaccc
    10381 aggccgttct atgattctgt cattctaaat ctctctttca gcctaaagct ttttccccgt
    10441 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
    10501 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
    10561 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
    10621 cgtaattaca tccctggggg ctttgggggg gggctgtccc tctagagtcg acggcgcgcc
    10681 ctgcaggaat ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    10741 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
    10801 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    10861 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
    10921 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    10981 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    11041 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
    11101 cccyccccmc ccccaatttt gtwtttattt attttttaat tattttgtgc agsgakgggg
    11161 gsgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
    11221 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
    11281 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggnagt
    11341 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
    11401 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
    11461 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
    11521 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
    11581 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
    11641 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
    11701 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
    11761 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
    11821 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
    11881 ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc
    11941 gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
    12001 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
    12061 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
    12121 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
    12181 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
    12241 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
    12301 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
    12361 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgattt gataccgcgg
    12421 gccccgcgtg gacggtaccg aagttcctat tctctagaaa gtataggaac ttccctcagc
    12481 accatggcta gcggcagcct cggagtttga atagatagaa taaaatatct ttattttcat
    12541 tccatctgtg tgttggtttt ttgtgtgaga tctacgggtg gcatccctgt gacccctccc
    12601 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
    12661 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
    12721 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
    12781 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
    12841 ttcaagcgat tctcctgcct caggctcccg agttgttggg attccaggca tgcatgacca
    12901 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
    12961 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
    13021 aggcgtgaac cactgctccc ttccctgtcc ttctgcctca gctaattgag taggggggag
    13081 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
    13141 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
    13201 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
    13261 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
    13321 tcggggcggc aggccgtgga agttcctatt ctctagaaag tataggaact tcacgcgcgc
    13381 gtccatttaa ttcgccgcca ccatgccacc caaaaagaaa agaaaggtgg tgagcaaggg
    13441 cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
    13501 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
    13561 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
    13621 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
    13681 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
    13741 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
    13801 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
    13861 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
    13921 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
    13981 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
    14041 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
    14101 cctgtacggc atggacgagc tatacaagta acaattgtgc ctcgactgtg ccttctagtt
    14161 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
    14221 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
    14281 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
    14341 ggcatgctgg ggacacgtgg gatccggtgc cagggcgtgc ccttgggctc cccgggcgcg
    14401 tttgggcggc cgcatcgaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
    14461 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
    14521 cacacattcc acatcctccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
    14581 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
    14641 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
    14701 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
    14761 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
    14821 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
    14881 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcaatcgccg
    14941 ctagccgcca ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
    15001 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
    15061 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
    15121 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
    15181 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
    15241 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
    15301 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
    15361 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
    15421 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgtaag tgctagcccc
    15481 taggcctgaa tcgaaaaacc gctcagcggc gcccgcaccc aaacgaaaac acccccggcc
    15541 caagcggacc gggggtgtcg cgtgaaatgg atcagtgagc gtggccgtgg tggtggccgt
    15601 ggccgtgctc gtcctcgtcg gccggcttgt cgacgacggc ggtctccgtc gtcaggatca
    15661 tccgggccac cgaggcggcg ttgagaacag ccgagcgggt caccttggcc ggatcgacga
    15721 cgccggcgga gaccagatca ccgaactcca gcgtggcggc gttgaagccc tgccccttgg
    15781 gaagttcgga gagagctcgt cgactgcaga ggcctgcatg caagcttggc gtaatcatgg
    15841 tcatagctgt ttccttatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    15901 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    15961 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    16021 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    16081 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    16141 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    16201 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    16261 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    16321 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    16381 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    16441 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    16501 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    16561 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    16621 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    16681 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    16741 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    16801 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    16861 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    16921 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    16981 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgc
//
