LOCUS       sequence-356684-       10239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human BTLA extracellular domain and transmembrane 
            domain fused to PD-1 intracellular domain followed by mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma
            Y, Wang H, Bennett EJ, Fu G, Hui E
  TITLE     PD-1 and BTLA regulate T cell signaling differentially and only 
            partially through SHP1 and SHP2.
  JOURNAL   J Cell Biol. 2020 Jun 1;219(6). pii: 151801. doi: 
            10.1083/jcb.201905085.
  PUBMED    32437509
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-06-1"; volume: "219(6). pii: 151801. doi"; 
            pages: " 10.1083/jcb.201905085"
COMMENT     Sequence Label: pHR-SV40-BTLAecto&tmd-PD-1icd-mGFP
FEATURES             Location/Qualifiers
     source          1..10239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(17..28)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(137..153)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(138..154)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             244..477
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(502..520)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(503..520)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(810..829)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1043..1062)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1162..1184
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1222..1240)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1308..1412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1413..1481,1482..2273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1631..1650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2444..3032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2933..2952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3186..3203
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3273..3293)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3278..3607
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3458..3593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4741..4806
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4936..4956
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5381..5515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5418..5437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5472..5491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5683..6316
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6363..6488
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6985..7218
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7403..7447
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7732..7849
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7907..8117
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7976..8111
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      8999..9008
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9005..9007,9008..9010,9011..9724)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9050..9071)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9311..9330)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9658..9679
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(9785..10239,1..134)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9838..9858)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
       61 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
      121 ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc tcggtacctt
      181 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
      241 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
      301 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      361 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      421 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
      481 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
      541 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
      601 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
      661 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
      721 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
      781 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
      841 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      901 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
      961 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     1021 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     1081 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     1141 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     1201 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1261 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2281 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2341 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2581 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3061 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3121 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3181 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3241 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     3301 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3361 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3421 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3481 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3541 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3601 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     3661 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     3721 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     3781 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     3841 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     3901 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     3961 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     4021 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     4081 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     4141 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     4201 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     4261 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     4321 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     4381 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     4441 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     4501 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     4561 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     4621 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     4681 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     4741 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     4801 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     4861 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     4921 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     4981 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     5041 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     5101 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     5161 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     5221 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     5281 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     5341 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     5401 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5461 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     5521 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     5581 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     5641 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     5701 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5761 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     5821 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     5881 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     5941 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     6001 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     6061 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     6121 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6181 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6241 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6301 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     6361 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     6421 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     6481 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     6541 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     6601 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     6661 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     6721 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     6781 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     6841 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6901 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6961 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     7021 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     7081 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     7141 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     7201 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     7261 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     7321 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     7381 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     7441 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     7501 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     7561 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7621 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     7681 attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag
     7741 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     7801 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     7861 cagggacagc agagatccag tttggatcga taagcttgat atcgaggcag gcagaagtat
     7921 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7981 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8041 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8101 ttttggaggc ctaggctgaa ttctcatgaa gacattgcct gccatgcttg gaactgggaa
     8161 attattttgg gtcttcttct taatcccata tctggacatc tggaacatcc atgggaaaga
     8221 atcatgtgat gtacagcttt atataaagag acaatctgaa cactccatct tagcaggaga
     8281 tccctttgaa ctagaatgcc ctgtgaaata ctgtgctaac aggcctcatg tgacttggtg
     8341 caagctcaat ggaacaacat gtgtaaaact tgaagataga caaacaagtt ggaaggaaga
     8401 gaagaacatt tcatttttca ttctacattt tgaaccagtg cttcctaatg acaatgggtc
     8461 ataccgctgt tctgcaaatt ttcagtctaa tctcattgaa agccactcaa caactcttta
     8521 tgtgacagat gtaaaaagtg cctcagaacg accctccaag gacgaaatgg caagcagacc
     8581 ctggctcctg tatcgtttac ttcctttggg gggattgcct ctactcatca ctacctgttt
     8641 ctgcctgttc tgctgcctgc gggccgcacg agggacaata ggagccaggc gcaccggcca
     8701 gcccctgaag gaggacccct cagccgtgcc tgtgttctct gtggactatg gggagctgga
     8761 tttccagtgg cgagagaaga ccccggagcc ccccgtgccc tgtgtccctg agcagacgga
     8821 gtatgccacc attgtctttc ctagcggaat gggcacctca tcccccgccc gcaggggctc
     8881 agccgacggc cctcggagtg cccagccact gaggcctgag gatggacact gctcttggcc
     8941 cctgacgcgt ctgcaggata tcaagcttgc ggtaccgcgg gcccgggatc caccggtcgc
     9001 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     9061 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     9121 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     9181 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     9241 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     9301 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     9361 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     9421 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     9481 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     9541 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     9601 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     9661 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     9721 gtaaagcggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     9781 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     9841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     9901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     9961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
    10021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
    10081 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
    10141 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
    10201 gctcgcctgt gttgccacct ggattctgcg cgggacgtc
//
