LOCUS       sequence-356687-       18985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18985)
  TITLE     Daigle Lab TIGRE3.0 plasmids 2022
REFERENCE   2  (bases 1 to 18985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP963-Ai193 (TICL-EGFP-ICF-tdT)
FEATURES             Location/Qualifiers
     source          1..18985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..79
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             98..901
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(161..180)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     771..790
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     930..952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     944..961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     945..961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1105..1154
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     enhancer        4377..4680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4682..4959
                     /label=chicken beta-actin promoter
     intron          4960..5976
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     protein_bind    complement(6070..6103)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6181..6229
                     /note="synthetic polyadenylation signal"
     polyA_signal    6238..6714
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    6726..7187
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(7214..7247)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7261..7270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7267..7269,7270..7272,7273..7986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7267..7269,7270..7272,7273..7983)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     7920..7941
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8011..8599
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8482..8493)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    8630..8837
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        11361..11664
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11666..11943
                     /label=chicken beta-actin promoter
     protein_bind    13050..13083
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    13129..13177
                     /note="synthetic polyadenylation signal"
     polyA_signal    13186..13662
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(13697..13716)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13741..13789
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    13949..13982
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      14007..14016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(14146..14164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     14414..14433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     14608..14631
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    15476..16064
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(15947..15958)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    16095..16302
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    16316..16349
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        16376..16875
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(16411..16434)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     16808..16827
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    17567..17616
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(17929..18517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18009..18028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgttttgg cggtttaaac attaagcacg tgttgacaat taatcatcgg catagtatat
       61 cggcatagta taatacgaca aggtgaggaa ctaaaccatg ggatcggcca ttgaacaaga
      121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
      181 acaacagacg atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
      301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
      361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
      421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
      481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
      541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
      601 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      721 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
      781 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
      841 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
      901 aagtgttaac tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      961 tgaattcgag ctcggtaccg aaaaaccgct cagcggcgcc cgcacccaaa cgaaaacacc
     1021 cccggcccaa gcggaccggg ggtgtcgcgt gaaatggatc agtgagcgtg gccgtggtgg
     1081 tggccgtggc cgtgctcgtc ctcgtcggcc ggcttgtcga cgacggcggt ctccgtcgtc
     1141 aggatcatcc gggccaccga ggcggcgttg agaacagccg agcgggtcac cttggccgga
     1201 tcgacgacgc cggcggagac cagatcaccg aactccagcg tggcggcgtt gaagccctgc
     1261 cccttgggaa gttcggagat ttcggccggc caatcgattt taccggggtg gtgcccatcc
     1321 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1381 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1441 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1501 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1561 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1621 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1681 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1741 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggtt
     1801 aattaagaat tcgagctcgc tagagcccca tcctcactga ctccgtcctg gagttggatg
     1861 agagataatg gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc
     1921 ttctccaaag agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc
     1981 cctggaggtg atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg
     2041 tgggtgggtt ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga
     2101 cgattgtatg aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca
     2161 gctgtatctc tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac
     2221 atgcaggctc agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc
     2281 tgggttttaa cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga
     2341 acgctgtgac ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct
     2401 gtcctgcact tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat
     2461 gtgtcacccc agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg
     2521 gctttacact gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga
     2581 gggtggtgac gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc
     2641 attcaaggcc aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca
     2701 catagcaggg ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat
     2761 gattctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt
     2821 gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc
     2881 cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc
     2941 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc
     3001 cctgggggct ttgggggggg gctgtccctc tagcgagctc ggtacccaga gccccatcct
     3061 cactgactcc gtcctggagt tggatgagag ataatggcct tacgttgtgc caggggaggg
     3121 tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt ggcacagctg
     3181 cccacggagg tgggggggtc accgtccctg gaggtgatga agaactgtgg ggatgtggca
     3241 ctgagggaca tggccagtgg gcacggtggg tgggttgggg ttggtcttgg ggatcttgga
     3301 gggcttttcc agccttcatg atttgacgat tgtatgaaca tctacatggc aattctccag
     3361 ctgcctgtcc cagtcctact gacccagctg tatctctcca ggcaagctct tccacccctt
     3421 ctgcttgcat ccagacacca tcaaacatgc aggctcagac acagggacca gcagtgtctg
     3481 tggccttttt gtgctcctct ccatgctggg ttttaacttg ctctttgtcc ttctatccta
     3541 tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc agagccctca
     3601 acacctgcat gtcccacgtc catgctgtcc tgcacttcct tatccccaag atctgcctct
     3661 ccgtgatgca ctgaattggc aaacatgtgt caccccagac caacaatgtc acagcaaact
     3721 cccccttgat aggacaaggg ggaatggctt tacactgaga caggggaggt ttgggttgga
     3781 tatgaggagg cagtttttcc cccagagggt ggtgacgcac tgaacaggtt gcccaaggag
     3841 gctgtggatg ccccatccct gcaggcattc aaggccaggc tggatgtggc tctgggcagc
     3901 ctgggctgct ggttgatgac cctgcacata gcagggggtt ggatctggat gagcactgtg
     3961 ctcctttgca acccaggccg ttctatgatt ctgtcattct aaatctctct ttcagcctaa
     4021 agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     4081 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
     4141 cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc
     4201 tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg tccctctagg
     4261 ggtacccggg gatccaccgg tccttaatta accgtttaaa caattctgca ggaatctagt
     4321 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4381 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4441 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4501 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4561 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4621 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4681 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     4741 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     4801 gggcgcgcgc crggsggggg ggggsggggs grggggsggg gsggggsgag gcggagaggt
     4861 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     4921 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     4981 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     5041 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     5101 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     5161 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     5221 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     5281 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     5341 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     5401 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     5461 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     5521 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     5581 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     5641 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     5701 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     5761 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     5821 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     5881 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     5941 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     6001 gtgctgtctc atcattttgg caaagaattg atttgatacc atttaaatgg ggatcaaatt
     6061 caattaaata taacttcgta tagcatacat tatacgaagt tattcgcgag tgaataaatg
     6121 aaagcttgca gatcccctca gcaccatggc tagcggcagc ctcggagttt gaatagatag
     6181 aataaaatat ctttattttc attccatctg tgtgttggtt ttttgtgtga gatctacggg
     6241 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     6301 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     6361 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     6421 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     6481 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcaggctcc cgagttgttg
     6541 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     6601 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     6661 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgcct
     6721 cagcgctgta agtctgcaga aattgatgat ctattaaaca ataaagatgt ccactaaaat
     6781 ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct acattttgaa
     6841 tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg cctgctcttt
     6901 actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat cataatttaa
     6961 acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta tagatctata
     7021 gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct aagtactgtg
     7081 gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc tgttccacat
     7141 acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaaagc ttttacttaa
     7201 ctgaatagct aacataactt cgtatagcat acattatacg aagttatcac gcgtgctagc
     7261 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     7321 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     7381 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     7441 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     7501 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     7561 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     7621 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     7681 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     7741 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     7801 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     7861 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     7921 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     7981 aagtaaacgc gttacgtaaa gcttgtcgat aatcaacctc tggattacaa aatttgtgaa
     8041 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8101 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8161 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8221 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8281 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8341 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8401 gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg
     8461 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8521 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8581 ctttgggccg cctccccgca tcgacaccga gcgctgctcg aggcctcgac tgtgccttct
     8641 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8701 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     8761 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8821 agcaggcatg ctggggacac gtgggatcct ctagagcccc atcctcactg actccgtcct
     8881 ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg ctggatttag
     8941 caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac ggaggtgggg
     9001 gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag ggacatggcc
     9061 agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct tttccagcct
     9121 tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc tgtcccagtc
     9181 ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct tgcatccaga
     9241 caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc tttttgtgct
     9301 cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc ttatccttaa
     9361 ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc tgcatgtccc
     9421 acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg atgcactgaa
     9481 ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc ttgataggac
     9541 aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga ggaggcagtt
     9601 tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt ggatgcccca
     9661 tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg ctgctggttg
     9721 atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct ttgcaaccca
     9781 ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
     9841 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     9901 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     9961 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
    10021 gtaattacat ccctgggggc tttggggggg ggctgtccct ctagagcccc atcctcactg
    10081 actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg
    10141 ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac
    10201 ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag
    10261 ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct
    10321 tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc
    10381 tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct
    10441 tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc
    10501 tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc
    10561 ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc
    10621 tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg
    10681 atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc
    10741 ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga
    10801 ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt
    10861 ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg
    10921 ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct
    10981 ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt
    11041 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
    11101 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
    11161 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
    11221 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct ctagagtcga
    11281 cggcgcgccc tgcaggaatc tagttattaa tagtaatcaa ttacggggtc attagttcat
    11341 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    11401 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    11461 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    11521 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    11581 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    11641 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
    11701 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
    11761 gsgatggggg sggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
    11821 cggggcgggg cgaggcggag aggkgcggcg gcagccaatc agagcggcgc gctccgaaag
    11881 tttcctttta tggcgaggsg gcggsggsgg sggccctata aaaagcgaag cgcgcggcgg
    11941 gsggggrgtc gctgcgncgc tgccttcgcc ccgtgccccg cyccgccgcc gcctcgcgcc
    12001 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
    12061 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
    12121 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
    12181 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
    12241 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
    12301 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
    12361 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
    12421 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
    12481 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
    12541 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
    12601 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
    12661 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
    12721 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
    12781 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
    12841 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
    12901 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
    12961 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgattt
    13021 gataccgcgg gccccgcgtg gacggtaccg aagttcctat tctctagaaa gtataggaac
    13081 ttccctcagc accatggcta gcggcagcct cggagtttga atagatagaa taaaatatct
    13141 ttattttcat tccatctgtg tgttggtttt ttgtgtgaga tctacgggtg gcatccctgt
    13201 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
    13261 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
    13321 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
    13381 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
    13441 gcctcctggg ttcaagcgat tctcctgcct caggctcccg agttgttggg attccaggca
    13501 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
    13561 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
    13621 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgcctca gctaattgag
    13681 taggggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
    13741 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
    13801 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
    13861 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
    13921 gcagccaacg tcggggcggc aggccgtgga agttcctatt ctctagaaag tataggaact
    13981 tcacgcgcgc gtccatttaa ttcgccgcca ccatggtgag caagggcgag gaggtcatca
    14041 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
    14101 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
    14161 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
    14221 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
    14281 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
    14341 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
    14401 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
    14461 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
    14521 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
    14581 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
    14641 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggta
    14701 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
    14761 tgattaagga gtttatgagg tttaaagtca ggatggaggg cagcatgaac gggcacgagt
    14821 ttgagattga aggagagggg gaagggaggc cctatgaggg cacccagaca gcaaagctga
    14881 aagtgacaaa gggcggccct ctgccattcg catgggacat cctgtctcct cagtttatgt
    14941 atggaagtaa agcctacgtg aaacaccctg ctgacattcc agattacaag aaactgagct
    15001 tcccagaggg gtttaagtgg gaaagagtga tgaacttcga agacgggggc ctggtgactg
    15061 tcacccagga cagctctctg caggacggca ctctgatcta taaggtgaaa atgcgcggca
    15121 ccaattttcc ccctgatggc ccagtcatgc agaagaagac aatgggctgg gaggcttcaa
    15181 cagaaaggct gtaccccaga gacggcgtgc tgaagggaga gatccaccag gcactgaagc
    15241 tgaaagatgg agggcattat ctggtcgaat tcaaaaccat ctacatggcc aagaaacccg
    15301 tgcagctgcc tggctactat tacgtcgaca caaagctgga tattactagc cataacgaag
    15361 actacaccat tgtggagcag tatgagcgga gcgaggggag gcaccatctg tttctgtacg
    15421 gcatggacga gctgtacaag taactaaggt taaccgttac gtaaagcttg tcgataatca
    15481 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
    15541 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
    15601 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    15661 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    15721 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    15781 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    15841 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta
    15901 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    15961 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    16021 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgac accgagcgct
    16081 gctcgaggcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    16141 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    16201 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    16261 caagggggag gattgggaag acaatagcag gcatgctggg gacacgtggg atccggtgcc
    16321 agggcgtgcc cttgggctcc ccgggcgcgt ttgggcggcc gcatcgaatt ctaccgggta
    16381 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
    16441 tggcgctaca caagtggcct ctggcctcgc acacattcca catcctccgg taggcgccaa
    16501 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
    16561 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
    16621 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
    16681 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
    16741 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
    16801 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
    16861 tctccgggcc tttcgacctg caatcgccgc tagccgccac catgaaaaag cctgaactca
    16921 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
    16981 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
    17041 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
    17101 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
    17161 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    17221 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
    17281 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    17341 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    17401 acgacaccgt cagtgtaagt gctagcccct aggcctgaat cgaaaaaccg ctcagcggcg
    17461 cccgcaccca aacgaaaaca cccccggccc aagcggaccg ggggtgtcgc gtgaaatgga
    17521 tcagtgagcg tggccgtggt ggtggccgtg gccgtgctcg tcctcgtcgg ccggcttgtc
    17581 gacgacggcg gtctccgtcg tcaggatcat ccgggccacc gaggcggcgt tgagaacagc
    17641 cgagcgggtc accttggccg gatcgacgac gccggcggag accagatcac cgaactccag
    17701 cgtggcggcg ttgaagccct gccccttggg aagttcggag agagctcgtc gactgcagag
    17761 gcctgcatgc aagcttggcg taatcatggt catagctgtt tccttatcag ctcactcaaa
    17821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    17881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    17941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    18001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    18061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    18121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    18181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    18241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    18301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    18361 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    18421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    18481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    18541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    18601 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    18661 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    18721 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    18781 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    18841 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    18901 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    18961 tagttcgcca gttaatagtt tgcgc
//
