LOCUS       sequence-356689-       11204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N-terminal mCherry tagged human SHP1 with its nSH2 and
            cSH2 replaced by nSH2 and cSH2 of SHP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Xu X, Masubuchi T, Cai Q, Zhao Y, Hui E
  TITLE     Molecular features underlying differential SHP1/SHP2 binding of 
            immune checkpoint receptors.
  JOURNAL   Elife. 2021 Nov 4;10. pii: 74276. doi: 10.7554/eLife.74276.
  PUBMED    34734802
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-11-4"; volume: "10. pii: 74276. doi"; pages: " 
            10.7554/eLife.74276"
COMMENT     Sequence Label: pHR-SV40-mCherry-SHP1 (SHP2-nSH2, cSH2)
FEATURES             Location/Qualifiers
     source          1..11204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(31..42)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(151..167)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(152..168)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             258..491
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(516..534)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(517..534)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(824..843)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1057..1076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1176..1198
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1236..1254)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1322..1426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1427..1495,1496..2287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1645..1664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2458..3046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2947..2966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3200..3217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3287..3307)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3292..3621
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3472..3607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4755..4820
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4950..4970
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5395..5529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5432..5451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5486..5505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5697..6330
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6377..6502
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6999..7232
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7417..7461
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7746..7863
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7921..8131
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7990..8125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8146..8853
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8291..8309)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8559..8578
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(10764..11204,1..148)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10817..10837)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
       61 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
      121 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc
      181 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
      241 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
      301 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      481 aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg
      541 tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag
      601 cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc
      661 gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg
      721 gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc
      781 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
      841 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
      901 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
      961 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     1021 tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata
     1081 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     1141 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     1201 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     1261 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1321 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1381 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1441 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1501 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1561 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1621 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1681 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1741 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1801 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1861 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1921 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1981 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2041 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2101 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2161 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2221 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2281 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2341 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2401 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag
     3301 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3361 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3421 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3481 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3541 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3601 ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag
     3661 aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga
     3721 aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa
     3781 cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt
     3841 cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga
     3901 acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg
     3961 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     4021 cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt
     4081 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     4141 cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt
     4201 gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg
     4261 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     4321 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     4381 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     4441 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat
     4501 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg
     4561 cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga
     4621 tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga
     4681 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     4741 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     4801 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     4861 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     4921 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca
     4981 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     5041 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     5101 tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt
     5161 tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt
     5221 gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct
     5281 tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     5341 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     5401 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5461 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5521 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     5581 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     5641 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     5701 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     5761 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     5821 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     5881 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     5941 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     6001 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     6061 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     6121 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     6181 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6241 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6301 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6361 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     6421 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     6481 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     6541 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     6601 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     6661 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     6721 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     6781 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     6841 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     6901 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     6961 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     7021 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     7081 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     7141 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     7201 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     7261 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     7321 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     7381 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     7441 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     7501 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     7561 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     7621 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     7681 gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc
     7741 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     7801 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     7861 tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgag
     7921 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7981 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8041 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8101 tagtgaggag gcttttttgg aggcctaggc tgaattctca cgcgtatggt gagcaagggc
     8161 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     8221 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     8281 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     8341 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     8401 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     8461 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     8521 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     8581 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     8641 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     8701 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     8761 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     8821 cactccaccg gcggcatgga cgagctgtac aagacgcgtg ggagttccgg tggtggcggg
     8881 agcggaggtg gaggctcgag cggtggcggc cgcatggtga ggtggtttca cccaaatatc
     8941 actggtgtgg aggcagaaaa cctactgttg acaagaggag ttgatggcag ttttttggca
     9001 aggcctagta aaagtaaccc tggagacttc acactttccg ttagaagaaa tggagctgtc
     9061 acccacatca agattcagaa cactggtgat tactatgacc tgtatggagg ggagaaattt
     9121 gccactttgg ctgagttggt ccagtattac atggaacatc acgggcaatt aaaagagaag
     9181 aatggagatg tcattgagct taaatatcct ctgaactgct ccgatcccac tagtgagagg
     9241 tggtttcatg gacatctctc tgggaaagaa gcagagaaat tattaactga aaaaggaaaa
     9301 catggtagtt ttcttgtacg agagagccag agccaccctg gagattttgt tctttctgtg
     9361 cgcactggtg atgacaaagg ggagagcaat gacggcaagt ctaaagtgac ccatgttatg
     9421 attcgctgtc aggaactgaa atacgacgtt ggtggaggag aacggtttga ttctttgaca
     9481 gatcttgtgg aacattataa gaagaatcct atggtggaaa cattgggtac agtactacaa
     9541 ctcaagcagc ccctttatgc cacgagggtg aatgcggctg acattgagaa ccgagtgttg
     9601 gaactgaaca agaagcagga gtccgaggat acagccaagg ctggcttctg ggaggagttt
     9661 gagagtttgc agaagcagga ggtgaagaac ttgcaccagc gtctggaagg gcagcggcca
     9721 gagaacaagg gcaagaaccg ctacaagaac attctcccct ttgaccacag ccgagtgatc
     9781 ctgcagggac gggacagtaa catccccggg tccgactaca tcaatgccaa ctacatcaag
     9841 aaccagctgc taggccctga tgagaacgct aagacctaca tcgccagcca gggctgtctg
     9901 gaggccacgg tcaatgactt ctggcagatg gcgtggcagg agaacagccg tgtcatcgtc
     9961 atgaccaccc gagaggtgga gaaaggccgg aacaaatgcg tcccatactg gcccgaggtg
    10021 ggcatgcagc gtgcttatgg gccctactct gtgaccaact gcggggagca tgacacaacc
    10081 gaatacaaac tccgtacctt acaggtctcc ccgctggaca atggagacct gattcgggag
    10141 atctggcatt accagtacct gagctggccc gaccatgggg tccccagtga gcctgggggt
    10201 gtcctcagct tcctggacca gatcaaccag cggcaggaaa gtctgcctca cgcagggccc
    10261 atcatcgtgc actgcagcgc cggcatcggc cgcacaggca ccatcattgt catcgacatg
    10321 ctcatggaga acatctccac caagggcctg gactgtgaca ttgacatcca gaagaccatc
    10381 cagatggtgc gggcgcagcg ctcgggcatg gtgcagacgg aggcgcagta caagttcatc
    10441 tacgtggcca tcgcccagtt cattgaaacc actaagaaga agctggaggt cctgcagtcg
    10501 cagaagggcc aggagtcgga gtacgggaac atcacctatc ccccagccat gaagaatgcc
    10561 catgccaagg cctcccgcac ctcgtccaaa cacaaggagg atgtgtatga gaacctgcac
    10621 actaagaaca agagggagga gaaagtgaag aagcagcggt cagcagacaa ggagaagagc
    10681 aagggttccc tcaagaggaa gtgagcggcc gcgactctag agtcgacctg caggcatgca
    10741 agcttgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
    10801 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
    10861 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
    10921 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
    10981 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
    11041 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
    11101 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
    11161 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gatt
//
