LOCUS       sequence-356690-        8496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 guide vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8496)
  TITLE     Daigle Lab TIGRE3.0 plasmids 2022
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP926-pX330-Batf3
FEATURES             Location/Qualifiers
     source          1..8496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        71..175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(176..244,245..1036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(394..413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1207..1795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1696..1715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1857..2105
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1857..1877
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2028..2047
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2127..2202
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        2299..2584
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          2852..3079
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      3091..3100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3100..3165
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3172..3192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3217..7317
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3217..7317
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7318..7365
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(7393..7410)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7399..7606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7615..7755
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7615..7744
                     /label=AAV2 ITR
     rep_origin      7830..8285
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7917..7936)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8127..8148
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8302..8321)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8421..8443
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(join(8481..8496,1..3))
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
       61 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      121 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      181 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      241 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      301 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      361 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      421 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      481 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      541 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
      601 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
      661 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
      721 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
      781 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
      841 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg
      901 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
      961 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1021 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1081 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1141 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1201 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1261 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1321 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1381 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1441 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1501 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1561 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1621 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1681 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1741 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1801 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtgagg
     1861 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     1921 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     1981 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     2041 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     2101 acaccgcttt gtccggctcg gaacctgttt tagagctaga aatagcaagt taaaataagg
     2161 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt tttagagcta
     2221 gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat tctgcagaca
     2281 aatggctcta gaggtacccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2341 caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc attgacgtca
     2401 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2461 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt gtgcccagta
     2521 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     2581 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     2641 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     2701 gggggggggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
     2761 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     2821 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     2881 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     2941 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga gcaagaggta
     3001 agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg agcacctgcc
     3061 tgaaatcact ttttttcagg ttggaccggt gccaccatgg actataagga ccacgacgga
     3121 gactacaagg atcatgatat tgattacaaa gacgatgacg ataagatggc cccaaagaag
     3181 aagcggaagg tcggtatcca cggagtccca gcagccgaca agaagtacag catcggcctg
     3241 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     3301 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     3361 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     3421 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     3481 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     3541 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     3601 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     3661 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     3721 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     3781 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     3841 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     3901 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     3961 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     4021 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     4081 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     4141 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     4201 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     4261 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     4321 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     4381 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     4441 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     4501 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     4561 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     4621 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     4681 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     4741 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     4801 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     4861 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     4921 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     4981 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     5041 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     5101 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     5161 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     5221 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     5281 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     5341 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     5401 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     5461 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     5521 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     5581 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     5641 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     5701 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     5761 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     5821 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     5881 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     5941 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     6001 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     6061 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     6121 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     6181 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     6241 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     6301 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     6361 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     6421 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     6481 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     6541 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     6601 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     6661 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     6721 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     6781 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     6841 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     6901 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     6961 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     7021 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     7081 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     7141 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     7201 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     7261 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaaa
     7321 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagtaaga attcctagag
     7381 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     7441 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     7501 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     7561 acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggagcgg ccgcaggaac
     7621 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     7681 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     7741 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7801 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     7861 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     7921 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7981 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8041 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8101 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8161 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     8221 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     8281 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     8341 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     8401 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     8461 gaaacgcgcg agacgaaagg gcctcgtgat acgcct
//
