LOCUS       sequence-356691-       11204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of N-terminal mCherry tagged human SHP1 with its cSH2 
            replaced by SHP2-cSH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Xu X, Masubuchi T, Cai Q, Zhao Y, Hui E
  TITLE     Molecular features underlying differential SHP1/SHP2 binding of 
            immune checkpoint receptors.
  JOURNAL   Elife. 2021 Nov 4;10. pii: 74276. doi: 10.7554/eLife.74276.
  PUBMED    34734802
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-11-4"; volume: "10. pii: 74276. doi"; pages: " 
            10.7554/eLife.74276"
COMMENT     Sequence Label: pHR-SV40-mCherry-SHP1 (SHP2-cSH2)
FEATURES             Location/Qualifiers
     source          1..11204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..55)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(40..56)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             146..379
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(404..422)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(405..422)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(712..731)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(945..964)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1064..1086
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1124..1142)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1210..1314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1315..1383,1384..2175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1533..1552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2346..2934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2835..2854
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3088..3105
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3175..3195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3180..3509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3360..3495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4643..4708
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4838..4858
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5283..5417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5320..5339)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5374..5393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5585..6218
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6265..6390
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6887..7120
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7305..7349
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7634..7751
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7809..8019
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7878..8013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8034..8741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8179..8197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8447..8466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(10652..11204,1..36)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10705..10725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11123..11134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgaggga
       61 attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
      121 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
      181 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      241 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      301 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      361 gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa
      421 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata
      481 tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt
      541 aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc
      601 cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg
      661 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
      721 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
      781 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
      841 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
      901 tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat
      961 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     1021 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     1081 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     1141 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1201 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1261 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1321 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1381 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1441 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1501 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1561 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1621 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1681 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1741 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1801 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1861 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1921 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1981 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2041 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2101 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2161 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2221 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2461 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2521 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2581 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2641 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2701 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2761 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2821 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2881 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2941 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3001 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3061 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3121 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg
     3181 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3241 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3301 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3361 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3421 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3481 gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata
     3541 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     3601 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     3661 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     3721 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     3781 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt
     3841 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
     3901 acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
     3961 tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc
     4021 caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact
     4081 atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat
     4141 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc
     4201 tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
     4261 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
     4321 acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt
     4381 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt
     4441 gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc
     4501 gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg
     4561 gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     4621 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     4681 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     4741 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     4801 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     4861 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     4921 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     4981 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     5041 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     5101 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     5161 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     5221 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     5281 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5401 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     5461 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     5521 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     5581 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     5641 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     5701 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     5761 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     5821 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     5881 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     5941 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     6001 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     6061 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6121 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6181 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6241 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6301 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6361 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6421 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6481 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6541 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6601 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6661 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6721 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     6781 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     6841 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     6901 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     6961 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     7021 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     7081 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     7141 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     7201 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     7261 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     7321 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     7381 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     7441 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     7501 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     7561 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc caaatggcag
     7621 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     7681 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     7741 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc gataagcttg
     7801 atatcgaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     7861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     7921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     7981 tccagaagta gtgaggaggc ttttttggag gcctaggctg aattctcacg cgtatggtga
     8041 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     8101 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     8161 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     8221 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     8281 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     8341 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     8401 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     8461 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     8521 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     8581 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     8641 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     8701 agggccgcca ctccaccggc ggcatggacg agctgtacaa gacgcgtggg agttccggtg
     8761 gtggcgggag cggaggtgga ggctcgagcg gtggcggccg catggtgagg tggtttcacc
     8821 gagacctcag tgggctggat gcagagaccc tgctcaaggg ccgaggtgtc cacggtagct
     8881 tcctggctcg gcccagtcgc aagaaccagg gtgacttctc gctctccgtc agggtggggg
     8941 atcaggtgac ccatattcgc atccagaact caggggattt ctatgacctg tatggagggg
     9001 agaagtttgc gactctgaca gagctggtgg agtactacac tcagcagcag ggtgtcctgc
     9061 aggaccgcga cggcaccatc atccacctca agtacccgct gaactgctcc gatcccacta
     9121 gtgagaggtg gtttcatgga catctctctg ggaaagaagc agagaaatta ttaactgaaa
     9181 aaggaaaaca tggtagtttt cttgtacgag agagccagag ccaccctgga gattttgttc
     9241 tttctgtgcg cactggtgat gacaaagggg agagcaatga cggcaagtct aaagtgaccc
     9301 atgttatgat tcgctgtcag gaactgaaat acgacgttgg tggaggagaa cggtttgatt
     9361 ctttgacaga tcttgtggaa cattataaga agaatcctat ggtggaaaca ttgggtacag
     9421 tactacaact caagcagccc ctttatgcca cgagggtgaa tgcggctgac attgagaacc
     9481 gagtgttgga actgaacaag aagcaggagt ccgaggatac agccaaggct ggcttctggg
     9541 aggagtttga gagtttgcag aagcaggagg tgaagaactt gcaccagcgt ctggaagggc
     9601 agcggccaga gaacaagggc aagaaccgct acaagaacat tctccccttt gaccacagcc
     9661 gagtgatcct gcagggacgg gacagtaaca tccccgggtc cgactacatc aatgccaact
     9721 acatcaagaa ccagctgcta ggccctgatg agaacgctaa gacctacatc gccagccagg
     9781 gctgtctgga ggccacggtc aatgacttct ggcagatggc gtggcaggag aacagccgtg
     9841 tcatcgtcat gaccacccga gaggtggaga aaggccggaa caaatgcgtc ccatactggc
     9901 ccgaggtggg catgcagcgt gcttatgggc cctactctgt gaccaactgc ggggagcatg
     9961 acacaaccga atacaaactc cgtaccttac aggtctcccc gctggacaat ggagacctga
    10021 ttcgggagat ctggcattac cagtacctga gctggcccga ccatggggtc cccagtgagc
    10081 ctgggggtgt cctcagcttc ctggaccaga tcaaccagcg gcaggaaagt ctgcctcacg
    10141 cagggcccat catcgtgcac tgcagcgccg gcatcggccg cacaggcacc atcattgtca
    10201 tcgacatgct catggagaac atctccacca agggcctgga ctgtgacatt gacatccaga
    10261 agaccatcca gatggtgcgg gcgcagcgct cgggcatggt gcagacggag gcgcagtaca
    10321 agttcatcta cgtggccatc gcccagttca ttgaaaccac taagaagaag ctggaggtcc
    10381 tgcagtcgca gaagggccag gagtcggagt acgggaacat cacctatccc ccagccatga
    10441 agaatgccca tgccaaggcc tcccgcacct cgtccaaaca caaggaggat gtgtatgaga
    10501 acctgcacac taagaacaag agggaggaga aagtgaagaa gcagcggtca gcagacaagg
    10561 agaagagcaa gggttccctc aagaggaagt gagcggccgc gactctagag tcgacctgca
    10621 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
    10681 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
    10741 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
    10801 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
    10861 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
    10921 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
    10981 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
    11041 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    11101 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    11161 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctc
//
