LOCUS       sequence-356695-       10595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human PD-1 G224A fused to mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Xu X, Masubuchi T, Cai Q, Zhao Y, Hui E
  TITLE     Molecular features underlying differential SHP1/SHP2 binding of 
            immune checkpoint receptors.
  JOURNAL   Elife. 2021 Nov 4;10. pii: 74276. doi: 10.7554/eLife.74276.
  PUBMED    34734802
REFERENCE   2  (bases 1 to 10595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-11-4"; volume: "10. pii: 74276. doi"; pages: " 
            10.7554/eLife.74276"
COMMENT     Sequence Label: pHR-PD-1 G224A-mGFP
FEATURES             Location/Qualifiers
     source          1..10595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(71..82)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(191..207)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             298..531
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(556..574)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(557..574)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(864..883)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1097..1116)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1216..1238
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1276..1294)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1362..1466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1467..1535,1536..2327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1685..1704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2498..3086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2987..3006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3240..3257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3327..3347)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3332..3661
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3512..3647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4795..4860
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4990..5010
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5435..5569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5472..5491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5526..5545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5737..6370
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6417..6542
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7039..7272
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7457..7501
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7786..7903
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8056..8463
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      9409..9418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9415..9417,9418..9420,9421..10134)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9460..9481)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9721..9740)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10068..10089
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(10195..10595,1..188)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10248..10268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
       61 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
      121 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
      181 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
      241 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
      301 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
      361 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      421 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      481 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
      541 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
      601 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
      661 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
      721 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
      781 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
      841 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      901 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      961 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     1021 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     1081 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     1141 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     1201 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     1261 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1321 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1381 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1441 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1501 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1561 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1621 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1681 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1741 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1801 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1861 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1921 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1981 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2041 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2101 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2161 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2221 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2281 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2341 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2641 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3121 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3241 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3301 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3361 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3421 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3481 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3541 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3661 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     3721 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     3781 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     3841 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     3901 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     3961 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     4021 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     4081 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     4141 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     4201 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     4261 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     4321 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     4381 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     4441 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     4501 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     4561 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     4621 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     4681 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     4741 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     4801 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     4861 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     4921 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     4981 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     5041 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     5101 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     5161 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     5221 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     5281 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     5341 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     5401 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     5461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     5581 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     5641 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     5701 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     5761 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     5821 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     5881 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     5941 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     6001 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     6061 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     6121 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     6181 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     6361 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     6421 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     6481 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     6541 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     6601 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     6661 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     6721 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     6781 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     6841 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     6901 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6961 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7021 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7081 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7141 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7201 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7261 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7321 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7381 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7441 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7501 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7561 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7621 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7681 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7741 gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg
     7801 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     7861 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     7921 cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa
     7981 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     8041 ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata
     8101 gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat
     8161 ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg
     8221 gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc
     8281 ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat
     8341 ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc
     8401 tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc
     8461 gggggggatc tggagctctc gagaattctc atgcagatcc cacaggcgcc ctggccagtc
     8521 gtctgggcgg tgctacaact gggctggcgg ccaggatggt tcttagactc cccagacagg
     8581 ccctggaacc cccccacctt ctccccagcc ctgctcgtgg tgaccgaagg ggacaacgcc
     8641 accttcacct gcagcttctc caacacatcg gagagcttcg tgctaaactg gtaccgcatg
     8701 agccccagca accagacgga caagctggcc gctttccccg aggaccgcag ccagcccggc
     8761 caggactgcc gcttccgtgt cacacaactg cccaacgggc gtgacttcca catgagcgtg
     8821 gtcagggccc ggcgcaatga cagcggcacc tacctctgtg gggccatctc cctggccccc
     8881 aaggcgcaga tcaaagagag cctgcgggca gagctcaggg tgacagagag aagggcagaa
     8941 gtgcccacag cccaccccag cccctcaccc aggccagccg gccagttcca aaccctggtg
     9001 gttggtgtcg tgggcggcct gctgggcagc ctggtgctgc tagtctgggt cctggccgtc
     9061 atctgctccc gggccgcacg agggacaata ggagccaggc gcaccggcca gcccctgaag
     9121 gaggacccct cagccgtgcc tgtgttctct gtggactatg cggagctgga tttccagtgg
     9181 cgagagaaga ccccggagcc ccccgtgccc tgtgtccctg agcagacgga gtatgccacc
     9241 attgtctttc ctagcggaat gggcacctca tcccccgccc gcaggggctc agccgacggc
     9301 cctcggagtg cccagccact gaggcctgag gatggacact gctcttggcc cctgacgcgt
     9361 ctgcaggata tcaagcttgc ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     9421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     9481 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     9541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     9601 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     9661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     9721 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     9781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     9841 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     9901 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     9961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
    10021 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
    10081 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
    10141 cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa
    10201 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
    10261 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
    10321 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
    10381 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
    10441 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
    10501 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
    10561 actgacaatt ccgtggtgtt gtcggggaaa tcatc
//
