LOCUS       sequence-356696-       10598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human BTLA with immunoreceptor tyrosine-based 
            inhibitory motif (ITIM) from PD-1, fused to mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Xu X, Masubuchi T, Cai Q, Zhao Y, Hui E
  TITLE     Molecular features underlying differential SHP1/SHP2 binding of 
            immune checkpoint receptors.
  JOURNAL   Elife. 2021 Nov 4;10. pii: 74276. doi: 10.7554/eLife.74276.
  PUBMED    34734802
REFERENCE   2  (bases 1 to 10598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-11-4"; volume: "10. pii: 74276. doi"; pages: " 
            10.7554/eLife.74276"
COMMENT     Sequence Label: pHR-BTLA (PD-1 ITIM)-mGFP
FEATURES             Location/Qualifiers
     source          1..10598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(62..78)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(63..79)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             169..402
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(427..445)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(428..445)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(735..754)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(968..987)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1087..1109
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1147..1165)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1233..1337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1338..1406,1407..2198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1556..1575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2369..2957
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2858..2877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3111..3128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3198..3218)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3203..3532
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3383..3518
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4666..4731
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4861..4881
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5306..5440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5343..5362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5397..5416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5608..6241
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6288..6413
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6910..7143
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7328..7372
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7657..7774
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7927..8334
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      9283..9292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9289..9291,9292..9294,9295..10008)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9334..9355)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9595..9614)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9942..9963
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(10069..10598,1..59)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10122..10142)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10540..10551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
       61 tcgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga ccaatgactt
      121 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
      181 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
      241 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      301 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      361 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc
      421 gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
      481 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
      541 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
      601 ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt
      661 catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      721 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      781 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      841 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      901 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
      961 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     1021 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     1081 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     1141 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1201 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1261 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1321 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1381 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1441 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1501 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1561 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1621 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1681 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1741 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1801 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1861 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1921 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1981 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2041 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2101 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2161 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2221 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2281 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2341 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2401 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2461 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2521 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2581 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2641 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2701 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2761 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2821 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2881 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2941 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3001 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3061 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3121 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3181 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3241 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3361 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3421 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3481 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
     3541 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
     3601 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
     3661 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     3721 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     3781 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     3841 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3901 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3961 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     4021 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     4081 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
     4141 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     4201 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
     4261 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     4321 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     4381 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     4441 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
     4501 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
     4561 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
     4621 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     4681 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     4741 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     4801 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     4861 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     4921 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     4981 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     5041 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     5101 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     5161 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     5221 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     5281 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     5341 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5401 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     5461 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     5521 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     5581 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     5641 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     5701 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     5761 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     5821 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     5881 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     5941 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     6001 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     6061 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6121 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6181 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6241 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     6301 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     6361 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     6421 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6481 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     6541 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     6601 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     6661 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     6721 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     6781 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     6841 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     6901 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     6961 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7021 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7081 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7141 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7201 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     7261 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     7321 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     7381 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     7441 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     7501 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     7561 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     7621 tcgacggtat cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     7681 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     7741 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     7801 tccagtttgg atcgataagc ttgatatcga attcctgcag ccccgataaa ataaaagatt
     7861 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     7921 gctgcagtaa cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc
     7981 agatcaaggg cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga
     8041 gcagtttcgg ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc
     8101 cgaggccaag aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc
     8161 atcagatgtt tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa
     8221 ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga
     8281 gctcacaacc cctcactcgg cgcgccagtc ctccgacaga ctgagtcgcc cgggggggat
     8341 ctggagctct cgagaattct catgaagaca ttgcctgcca tgcttggaac tgggaaatta
     8401 ttttgggtct tcttcttaat cccatatctg gacatctgga acatccatgg gaaagaatca
     8461 tgtgatgtac agctttatat aaagagacaa tctgaacact ccatcttagc aggagatccc
     8521 tttgaactag aatgccctgt gaaatactgt gctaacaggc ctcatgtgac ttggtgcaag
     8581 ctcaatggaa caacatgtgt aaaacttgaa gatagacaaa caagttggaa ggaagagaag
     8641 aacatttcat ttttcattct acattttgaa ccagtgcttc ctaatgacaa tgggtcatac
     8701 cgctgttctg caaattttca gtctaatctc attgaaagcc actcaacaac tctttatgtg
     8761 acagatgtaa aaagtgcctc agaacgaccc tccaaggacg aaatggcaag cagaccctgg
     8821 ctcctgtatc gtttacttcc tttgggggga ttgcctctac tcatcactac ctgtttctgc
     8881 ctgttctgct gcctgagaag gcaccaagga aagcaaaatg aactctctga cacagcagga
     8941 agggaaatta acctggttga tgctcacctt aagagtgagc aaacagaagc aagcaccagg
     9001 caaaattccc aagtactgct atcagaaact ggaatttatg ataatgaccc tgacctttgt
     9061 ttcaggatgc aggaagggtc tgaagtttat tctaatccat gcctggaaga aaacaaacca
     9121 ggcgtggact atggggagct gaaccattct gtcattggac tgaactcaag actggcaaga
     9181 aatgtaaaag aagcaccaac agaatatgca tccatatgtg tgaggagtac gcgtctgcag
     9241 gatatcaagc ttgcggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
     9301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     9361 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     9421 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     9481 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     9541 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     9601 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     9661 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     9721 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     9781 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     9841 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     9901 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     9961 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
    10021 tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
    10081 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    10141 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    10201 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    10261 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    10321 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    10381 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    10441 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
    10501 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    10561 cttccttccc gcggcctgct gccggctctg cggcctct
//
