LOCUS       sequence-356718-        8994 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8994)
  TITLE     Daigle Lab TIGRE3.0 plasmids 2022
REFERENCE   2  (bases 1 to 8994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP940-Sncg-IRES2-FlpO-neo
FEATURES             Location/Qualifiers
     source          1..8994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1716..2289
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1739..2291
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1883..1900)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2110..2129
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      2303..2312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2309..2314,2315..2335,2336..3607)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     complement(3615..3632)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3621..3828
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3882..3915
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        3942..4441
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3977..4000)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4374..4393
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4458..4505
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4524..5327
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4587..4606)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5197..5216
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5337..5798
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     primer_bind     complement(6806..6826)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6806..6824)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6845..6861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6845..6861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6858..6880)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6869..6885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6893..6899,6900..6917,6918..6923))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6938..6959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7076..7093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7247..7835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7327..7346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8006..8797,8798..8866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASQQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8629..8648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8867..8971)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
      661 cgccggcgtt atccagaagc gatcgcgtcg acatttaaat ggccggccgg ctttgccatt
      721 ttctgttgac ccatgaacta gttgtgacat cataccctaa ccccaagtta acacagatac
      781 gttctgagaa cagactctag actgaccatc agtctagttc caaacccact gccgtcatga
      841 ccgtgaagga aatgctgtgg agatggaaga caggcctgag ccaagagtcc atccggaagg
      901 ccggaagagt gacccacact cctgagtacc tgagagggac ctgaagccct gatactttga
      961 actctgaagc catgcaatag tatctaggcc ctgaccccta tcctcaggat ctctgtgaac
     1021 agtgatggca ttcgatgctc atacagcctg agggaggggt ttgtgtgact caagggtctc
     1081 tccttactgc acacactcca ggcttcctcg ctttaggagc ctaagtctgt aggggccagg
     1141 tgcccatgac ttgggatgtg cttgcaggag atgatgcctg gcttggaggg tatctggcac
     1201 ctccagaccc tctaagcaca gaaacatgat ggaaggcctc tgttcatctg tgagacctga
     1261 caggggagac cctgctatgg caactgaggg cccgtgtctc ttcccatagc cttgtgtctc
     1321 ctttgctccc ctgtaggagg acttggaacc ccctgcacag gaccaagaag ccaaagagca
     1381 agaggagaat gaagaggtaa gaggggttcc cagggtgcac cagaaagttc tgccatagag
     1441 acctgagcca gagaggccct gctttcattt cttggtcctg ggagaccttc cacagcttcc
     1501 cacagtgccg tgggtagggc agtcagggac ccgagtgcct gaccgctcca tgagtgacca
     1561 tttccaggtg gtcaaggaga attgtgggcc tggaaatggg tgcaagtctc tttccctccc
     1621 tccttccttc ccttcctccc tccctcctgc cctccccctc ttcttcctgc cctctcttct
     1681 ctgccaggcc aagagtggag aagactagct taaggcccct ctccctcccc cccccctaac
     1741 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     1801 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     1861 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     1921 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     1981 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     2041 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     2101 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     2161 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     2221 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     2281 cgatgataat atggcgaccg cggccaccat ggctcctaag aagaagagga aggtgatgag
     2341 ccagttcgac atcctgtgca agaccccccc caaggtgctg gtgcggcagt tcgtggagag
     2401 attcgagagg cccagcggcg agaagatcgc cagctgtgcc gccgagctga cctacctgtg
     2461 ctggatgatc acccacaacg gcaccgccat caagagggcc accttcatga gctacaacac
     2521 catcatcagc aacagcctga gcttcgacat cgtgaacaag agcctgcagt tcaagtacaa
     2581 gacccagaag gccaccatcc tggaggccag cctgaagaag ctgatccccg cctgggagtt
     2641 caccatcatc ccttacaacg gccagaagca ccagagcgac atcaccgaca tcgtgtccag
     2701 cctgcagctg cagttcgaga gcagcgagga ggccgacaag ggcaacagcc acagcaagaa
     2761 gatgctgaag gccctgctgt ccgagggcga gagcatctgg gagatcaccg agaagatcct
     2821 gaacagcttc gagtacacca gcaggttcac caagaccaag accctgtacc agttcctgtt
     2881 cctggccaca ttcatcaact gcggcaggtt cagcgacatc aagaacgtgg accccaagag
     2941 cttcaagctg gtgcagaaca agtacctggg cgtgatcatt cagtgcctgg tgaccgagac
     3001 caagacaagc gtgtccaggc acatctactt tttcagcgcc agaggcagga tcgaccccct
     3061 ggtgtacctg gacgagttcc tgaggaacag cgagcccgtg ctgaagagag tgaacaggac
     3121 cggcaacagc agcagcaaca agcaggagta ccagctgctg aaggacaacc tggtgcgcag
     3181 ctacaacaag gccctgaaga agaacgcccc ctaccccatc ttcgctatca agaacggccc
     3241 taagagccac atcggcaggc acctgatgac cagctttctg agcatgaagg gcctgaccga
     3301 gctgacaaac gtggtgggca actggagcga caagagggcc tccgccgtgg ccaggaccac
     3361 ctacacccac cagatcaccg ccatccccga ccactacttc gccctggtgt ccaggtacta
     3421 cgcctacgac cccatcagca aggagatgat cgccctgaag gacgagacca accccatcga
     3481 ggagtggcag cacatcgagc agctgaaggg cagcgccgag ggcagcatca gataccccgc
     3541 ctggaacggc atcatcagcc aggaggtgct ggactacctg agcagctaca tcaacaggcg
     3601 gatctgacgt acggcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3661 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3721 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     3781 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggagg cgcgccgtaa
     3841 aagcttggca tagatcttat tgcagaagcc atatgtttta ggtgccaggg cgtgcccttg
     3901 ggctccccgg gcgcgtttgg acgcatttat cgaattctac cgggtagggg aggcgctttt
     3961 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcccttggc gctacacaag
     4021 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     4081 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     4141 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     4201 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     4261 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     4321 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     4381 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     4441 gacctgcagc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     4501 acgacaaggt gaggaactaa accatgggat cggccattga acaagatgga ttgcacgcag
     4561 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacgatcg
     4621 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4681 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     4741 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4801 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4861 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4921 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4981 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5041 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5101 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5161 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5221 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5281 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaggg gatccgctgt
     5341 aagtctgcag aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt
     5401 tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat
     5461 tggagctacg ggggtggggg tggggtggga ttagataaat gcctgctctt tactgaaggc
     5521 tctttactat tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa
     5581 accaaattaa gggccagctc attcctccca ctcatgatct atagatctat agatctctcg
     5641 tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa
     5701 tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat
     5761 tctcagtatt gttttgccaa gttctaattc catcagaaag cttaccccaa ctggggtaac
     5821 ctttgagttc tctcagttgg gggagcggcc gcgctagctg tacaaatatt aaggctgcca
     5881 gccaaccaca gaagttaatg gaacacccct ctgccttgga ccctgtctca acctggcaca
     5941 ctgaatgccc tgcctagagt gccctgcagg ttgcccaagt tctctgtccc tagcccagtg
     6001 ccacaagtcc acacacgcta ctgccagcgc cagcaccccc tgctggctcc ttttacccta
     6061 tggtcctctg atcaccaatc agatgctgct gtgatttctt tttttttaat gattccaaat
     6121 aaaacctgag ccccactccc accagtgctt cttctttgtg ctatgccgac cctcatgaag
     6181 ccctctggac attcccaggg gaaagacatc aaggctcagc atctgatggg accaggtaca
     6241 cgtgaagagt tctggaacct gtgccttcag gagccctgct gtctttagca gcagtgtatc
     6301 atcctttctc ttggctcccc ctaaataaca ggctgctgat actgtccatg gaagtgcagt
     6361 ggctagacag ggtctcagac tcagtgtccc tttagagcgg atcccactgc ctgcctggtt
     6421 cactgtgtct gaggaacctc aggtgggcag agggtgagaa tgtctgtgta actgcagctt
     6481 gtgtgttacg gggaccaggg cttcaaccac agggtgaaca gggacagcag agcaacccaa
     6541 ggcaccttcc atggcccctg acatcctcag acaggtgggc tttcctttcc cctctagccc
     6601 aggagactac aggacatcat aaagaaaatg gtgttgacat atttgggact gggagtatag
     6661 tttggtggca gaatgcttgc ctaccaagca tgaggccgta ggttcaaacc tcagtgctga
     6721 agatgaataa atagctggta tgcaccatgg taagcctcga ggtttaaact taattaacac
     6781 cgcggtggag ctccagcttt tgtccccttt agtgagggtt aattgcgcgc ttggcgtaat
     6841 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6901 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6961 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7021 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagtt cactcaaagg
     7141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7681 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7921 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7981 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8041 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8101 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8161 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8221 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ctgggaagct agagtaagta
     8281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8581 aatagtgtat gcggcgaccg agctgctctt gcccggcgtc aatacgggat aataccgcgc
     8641 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8701 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8761 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8821 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcataccc ttcctttttc
     8881 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8941 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
