LOCUS       sequence-356725-       10011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10011)
  TITLE     Daigle Lab TIGRE3.0 plasmids 2022
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP936-Chrna6-IRES2-FlpO-WPRE-neo
FEATURES             Location/Qualifiers
     source          1..10011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1816..2389
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1839..2391
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1983..2000)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2210..2229
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      2403..2412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2409..2414,2415..2435,2436..3707)
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPo
                     /note="FLPo is a mouse codon-optimized fusion between the 
                     SV40 nuclear localization signal and a thermostable version
                     of the Saccharomyces cerevisiae site-specific recombinase 
                     FLP.  Recombination occurs at FRT sequences."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     misc_feature    3724..4312
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3777..3797)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4195..4206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4330..4347)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4336..4543
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4597..4630
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        4657..5156
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4692..4715)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5089..5108
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        5173..5220
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5239..6042
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5302..5321)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5912..5931
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6052..6513
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     primer_bind     complement(7823..7843)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7823..7841)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7862..7878)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7862..7878)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7875..7897)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7886..7902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7910..7916,7917..7934,7935..7940))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7955..7976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8093..8110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8264..8852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8344..8363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9023..9814,9815..9883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASQQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9646..9665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9884..9988)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
      661 cgccggcgtt atccagaagc gatcgcgtcg acatttaaat ggccggccga agccccagtg
      721 gtgaaagatg ccccttagca gtcagcagct cccatcacac ttcctctact agagggggac
      781 ccagacatga gttaaagaca tcttgaagat gtgctcagta tgcacacatt ccgtgcctgt
      841 gtcccacact ctaatgccta gagcttggct cctcctaccc caagcaagtg ctcaaggata
      901 gggagatcac cacttgactg aggtttcccg ccaggtgtct ggagatgctt catactatag
      961 ctcctaacat ctgtaactca tagggaaaga atatcagaac caccaccacc ccccaaaaaa
     1021 cacactgaga gacactgaga atgcctgtca taacatgtca caggagagca atggccagtg
     1081 agctcacctc ctggctggca tcttttagaa tgtaggcaat acagttactg ttgatttatc
     1141 aggccaggca ggtctcctaa tacacaaccc acagagacca tagcagaagg ctagaagttt
     1201 gaaactggaa agattccaga tatgcaataa gctctctttg ttagcatccc ttagcagcct
     1261 agaggctgct tagtcctatg caggagatcc tccaccgtac cccacatcct ctggcctatc
     1321 ctctcacttg cttcacactc agattccccc aaacagccag gcacacgtga caagagcttg
     1381 actctcaagc agatattttg tggagagcga agaaaggacc cttatcacag gcaaagggtc
     1441 acaaaagtct ttgatacagt tcatagccca gtgatgtaca tctgctctcc ttagacaatg
     1501 agtcctacat aggacatgca ggcagtcagg caggcaaaca taatagcaat aattggtttt
     1561 tttgaaagga ttggagaaaa tgctttggag aaaaaaaaaa gtattcataa aacatagcat
     1621 gttttcttat agtcttggtt tttaaagcta gccttgatcc tttgtttgca tttcaggtag
     1681 aagacgactg gaaatacatg gctatggtgg tggacagagt cttcctttgg gtatttataa
     1741 ttgtctgtgt gtttggaact gtggggctat ttctgcagcc actgcttggg aacacaggaa
     1801 agtcttaact taaggcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     1861 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     1921 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     1981 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     2041 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     2101 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     2161 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     2221 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     2281 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     2341 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggcgaccg
     2401 cggccaccat ggctcctaag aagaagagga aggtgatgag ccagttcgac atcctgtgca
     2461 agaccccccc caaggtgctg gtgcggcagt tcgtggagag attcgagagg cccagcggcg
     2521 agaagatcgc cagctgtgcc gccgagctga cctacctgtg ctggatgatc acccacaacg
     2581 gcaccgccat caagagggcc accttcatga gctacaacac catcatcagc aacagcctga
     2641 gcttcgacat cgtgaacaag agcctgcagt tcaagtacaa gacccagaag gccaccatcc
     2701 tggaggccag cctgaagaag ctgatccccg cctgggagtt caccatcatc ccttacaacg
     2761 gccagaagca ccagagcgac atcaccgaca tcgtgtccag cctgcagctg cagttcgaga
     2821 gcagcgagga ggccgacaag ggcaacagcc acagcaagaa gatgctgaag gccctgctgt
     2881 ccgagggcga gagcatctgg gagatcaccg agaagatcct gaacagcttc gagtacacca
     2941 gcaggttcac caagaccaag accctgtacc agttcctgtt cctggccaca ttcatcaact
     3001 gcggcaggtt cagcgacatc aagaacgtgg accccaagag cttcaagctg gtgcagaaca
     3061 agtacctggg cgtgatcatt cagtgcctgg tgaccgagac caagacaagc gtgtccaggc
     3121 acatctactt tttcagcgcc agaggcagga tcgaccccct ggtgtacctg gacgagttcc
     3181 tgaggaacag cgagcccgtg ctgaagagag tgaacaggac cggcaacagc agcagcaaca
     3241 agcaggagta ccagctgctg aaggacaacc tggtgcgcag ctacaacaag gccctgaaga
     3301 agaacgcccc ctaccccatc ttcgctatca agaacggccc taagagccac atcggcaggc
     3361 acctgatgac cagctttctg agcatgaagg gcctgaccga gctgacaaac gtggtgggca
     3421 actggagcga caagagggcc tccgccgtgg ccaggaccac ctacacccac cagatcaccg
     3481 ccatccccga ccactacttc gccctggtgt ccaggtacta cgcctacgac cccatcagca
     3541 aggagatgat cgccctgaag gacgagacca accccatcga ggagtggcag cacatcgagc
     3601 agctgaaggg cagcgccgag ggcagcatca gataccccgc ctggaacggc atcatcagcc
     3661 aggaggtgct ggactacctg agcagctaca tcaacaggcg gatctgacgt acggcttgtc
     3721 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3781 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3841 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3901 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3961 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4021 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4081 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4141 ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4201 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4261 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgacac
     4321 cgacgcgtgc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4381 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4441 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4501 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggaggcgcgc cgtaaaagct
     4561 tggcatagat cttattgcag aagccatatg ttttaggtgc cagggcgtgc ccttgggctc
     4621 cccgggcgcg tttggacgca tttatcgaat tctaccgggt aggggaggcg cttttcccaa
     4681 ggcagtctgg agcatgcgct ttagcagccc cgctgggccc ttggcgctac acaagtggcc
     4741 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     4801 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     4861 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     4921 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     4981 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     5041 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     5101 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     5161 gcagcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     5221 aaggtgagga actaaaccat gggatcggcc attgaacaag atggattgca cgcaggttct
     5281 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac gatcggctgc
     5341 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5401 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     5461 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5521 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5581 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5641 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5701 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5761 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5821 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5881 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5941 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6001 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatcc gctgtaagtc
     6061 tgcagaaatt gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg
     6121 tcatactttg ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag
     6181 ctacgggggt gggggtgggg tgggattaga taaatgcctg ctctttactg aaggctcttt
     6241 actattgctt tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa
     6301 attaagggcc agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga
     6361 tcattgtttt tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt
     6421 cagtttcata gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca
     6481 gtattgtttt gccaagttct aattccatca gaaagcttac cccaactggg gtaacctttg
     6541 agttctctca gttgggggag cggccgcgct agcttggtat tgtcccttta ccttctgaat
     6601 cttttagatg ttgtgttttt taccaaactt gtacccacaa gaaaaagcca agtacacaga
     6661 ctcctaaacc aagacaagaa gggagtgggc attgctgcag ccttaggagc ctgaatacct
     6721 gtcttcctta cactgggtgt tttatcacct tggagcccag agctggcagt tgtcctaggc
     6781 taggaggact gagtcagctt ctctataatt tgcattcttt gtttcaagaa acacaaatgg
     6841 tccctcgtag agctaggccc catagtcaag agtcagtcca ctctgaaagg tgattggctt
     6901 gtatccgaag ctcctgctgg ttattgcaag tgatgtgtat ggttatcaag aaactcggga
     6961 gcgctgctga cacttgcaac acacctgtct gctactttct tgtgcatgtc cctgtgatgt
     7021 agctcacact catatctgac atgtgcaatg gtcaaagaag aaagcagata gcatctctct
     7081 tagcagacta ttcaattcgt ggtgcaatgg gttttctgtc cgtgtgaaaa tcactgaact
     7141 gataggcgta aaaggatgac gtttatatga gctcatggat ttggaggact ctgcccatga
     7201 tcactaggtc ccattgcctc tgggcctttg atgaggcaaa gcatcatgcc tagaagagga
     7261 tggctgccca cttaaagata gctacaaaga aagcaaacag agaggaaggc tctggagtgc
     7321 caatattcct ttcacagtca cagcccaatg acttcatgtc ttcctacaag accttccatc
     7381 ctagaagctc cactacctcc tgatagcgtg gaggaatcag tctctaacac ttgtccttgg
     7441 ggctgatcca aaccacagca gtattgtcct ggcaggacat ttggctggct ccagtactgt
     7501 gctaggtgct acaaggggat acataagagt ctttccctta taaagttaca gccttttaga
     7561 aaagccgtat atgcaaagag ccacaggatc aggtagtaga ggatatagct agagcaaccc
     7621 aaatgggaga aactaatcgg acaaacctaa actcactctt gtgtttcaaa cagatatctg
     7681 tgtagctttt tcccacgagc ccttctctag ctcactgcat ggagatgaaa taaaatcgat
     7741 atgaaatttt aatcttgtgc catttggagc catctcgagt ttaaacttaa ttaacaccgc
     7801 ggtggagctc cagcttttgt cccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     7861 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7921 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7981 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8041 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8101 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagttcac tcaaaggcgg
     8161 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8221 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8281 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8341 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8401 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8461 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8521 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8581 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8641 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8701 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8761 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8821 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8881 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8941 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9001 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9061 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9121 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9181 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9241 caactttatc cgcctccatc cagtctatta attgttgctg ggaagctaga gtaagtagtt
     9301 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9361 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9421 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9481 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9541 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9601 agtgtatgcg gcgaccgagc tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9661 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9721 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9781 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9841 caaaaaaggg aataagggcg acacggaaat gttgaatact catacccttc ctttttcaat
     9901 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9961 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
