LOCUS       sequence-356730-       14143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MUM2 protein fused to Citrine, under control of native promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14143)
  AUTHORS   Lee YC, Dean GH, Gilchrist E, Tsai AY, Haughn GW
  TITLE     Asymmetric distribution of extracellular matrix proteins in seed 
            coat epidermal cells of Arabidopsis is determined by polar 
            secretion.
  JOURNAL   Plant Direct. 2021 Nov 28;5(11):e360. doi: 10.1002/pld3.360. 
            eCollection 2021 Nov.
  PUBMED    34877448
REFERENCE   2  (bases 1 to 14143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2021-11-28"; volume: "5(11):e360. doi"; pages: " 
            10.1002/pld3.360. eCollection 2021 Nov"
COMMENT     Sequence Label: gMUM2-Citrine
FEATURES             Location/Qualifiers
     source          1..14143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    625..647
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(671..923)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(943..1494)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1536..1715)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1947..1969
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1961..1978
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1962..1978
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1988..2007
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1988..2006
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(9467..9469,9470..10180)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9509..9530)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9770..9789)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10117..10138
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(10496..10516)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10496..10514)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10535..10551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10535..10551)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10548..10570)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10559..10575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10583..10589,10590..10607,10608..10613))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10628..10649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10766..10783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10888..10912
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(11003..11591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11083..11102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13100..13915)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(13100..13915)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     13823..13842
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     13907..13926
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcct ttgcccacaa cccggcggcc ggccgcaaca gatcgtttta
      541 taaatttttt tttttgaaaa agaaaaagcc cgaaaggcgg caacctctcg ggcttctgga
      601 tttccgatcc ccggaattag atcttggcag gatatattgt ggtgtaacgt tatcagcttg
      661 catgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
      721 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
      781 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
      841 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
      901 tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt cggtgacggg
      961 caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg
     1021 ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc
     1081 ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa
     1141 gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca gtcccgtccg
     1201 ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc
     1261 cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca
     1321 gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc
     1381 ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg
     1441 catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcatggtaga
     1501 tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1561 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1621 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1681 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1741 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1801 taacattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     1861 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     1921 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     1981 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgagatatt
     2041 tatattgtga cttgtaagtg gactggtcag agtattactg tgagcttctc cgcagcggat
     2101 ttcttctctg ttcatgtaat tgcatacgtc attgagaata gatgacttca tcttagtcac
     2161 acgccggctt atatgtatac gtaaagtctg aaagatgcga taatcgacca gtttcgtgga
     2221 ccaaattttc cacataaacg acagttacta tttaaaatga aatgtattat agtttgctta
     2281 ttcttttccc taatatttta taaaatgttg tttagtaaca agtactaaga ccatcttcat
     2341 tggtaatctt taagagtttc taagcacaaa tccttatgaa aaaagtttaa aaataattca
     2401 aaaatgtgaa attaagcaag gtatgcacct tagcttaggt attctttggt ttctaagaaa
     2461 cttatatgtg gcagtttttc attgactgga gaagaataca aaacaaaaca taaaatcttt
     2521 cacatttttt ttatatcttc tgaagataaa tttgaaaata aatctaattg cttttggttt
     2581 cttttaattg aatagatagc aaaaatatta tctgagtcaa atttaaattt aatcgttatt
     2641 ccaatttttt atttgtcttt ttatatgttt ttatgtcata tatgtttatg atttaagaat
     2701 ttttaataat tattatgctt tatcaaatcg aaatacttat gatttttcat tttaaaacta
     2761 actttttttc tttaaagaaa cccacttaag gattccacca ttggacaata aaaatagtaa
     2821 agactcaaca aaatctctaa acttcaaaat aatcaaaatt tattcttaaa aaaattaaag
     2881 gattccacat taggactcca ccaatggggg tgctctaatg aatgtaagta aactgatctt
     2941 atatgttata atttatattg gggtatgttt gacaactgtg acaattagaa aaatgggctt
     3001 tttgagaaga aaaagaatgt aaaactcaat ggaagatata aaaatggctc ccaaaatcat
     3061 aaaacatacg aaaaatattt atgtgtgttt agaaacatat atcatgttta ataaccaaaa
     3121 aagaagaaga agaagatatt tataaactag aaaagaaaag gtaatgctta gctagagcat
     3181 caagattgta aaagcccata tggggcttaa ctataacttc ccttttaaag gcccatttta
     3241 tctttacgtg aaggctgtgt tgggtccaag tcttgtcttc caagttccag ttgcaatgtc
     3301 gtatacgcaa aacctcaaca cctaggtttc aactttttcc caaattttgc ttagacaagt
     3361 gtatatgcaa aatgaagaac tttaatcgaa taacttttct tgtcaataat tttttttttt
     3421 ttttttgctt taaggtttta attaacctat ttaaaataca atttgatatg ttgaaattta
     3481 aaacatatat tgtaaaaata aatttacaag aaatctttac aaaacaagat tggaccattt
     3541 tgttgttttg aaaaatattc aacttgctca tttttagcat tgacttgaac ttattcaaca
     3601 taagtatcac ttaatcactt tactaattca actgatcaaa acttcttttt tggtgggata
     3661 ttcaactgat caaaacagta aaggttgatt attagtttgc cttagaagtt gaagatcata
     3721 tagatgctag tcaaattaga aggggcattt tggtaattca gttgtagaat cgtaagacaa
     3781 aggtgaaaag cataatgcta ttttcaggat tatataacac atcaacagag gatccaaact
     3841 tttcccaatt attgcaagtc aatcgatttg tttatctata taaagctccc aaatctcaaa
     3901 tctcaaatct caaatttttt attaaacgcc tacatatatt actcacactt gttttcagat
     3961 cataaagttt tttcttggat ttatatataa tgcactaata catacacacc cacatgctta
     4021 cacacatgtt cgaactgtaa tcagtaaaag aaacatcaga gaggaagaga aggagaagaa
     4081 aagatggaga tgggtcgtct ggtcttcggg ttgtgtttga ttcttattgt gggtacgttt
     4141 cttgaattct ccggcggtgc tacggcggcg aagggagtaa cgtacgacgg acggtctttg
     4201 ataatcgatg gacagaggaa gcttctcttc tccggttcca tccactatcc tcgcagcaca
     4261 cccgaggtaa atgtttccat catacataaa ctagatgtga tttaatgtga taataacatt
     4321 tcggcaaacc tctttaaccc aaacattttt ggcaaatgaa ccttttatca aattaaaatg
     4381 gtaaacattt ttggcaaacg taaataatta tggttggacc ataaacttgt gtcatatcaa
     4441 ctattgatgt aatatgacaa aactaagcta tcaaaaaaca aaactatacg tatatgacaa
     4501 aatcaggagt ataattttgg atatatatga aagcaatgtt ttgttgataa gatctaaaat
     4561 catatgctag atattgctgc aaaactatgt atcgtagtcg acctaattga tatatatgat
     4621 gtaatgtaca tctatatact actactttct aacgaattct gtttcacgag tttctttgca
     4681 tttaaaattt ggtttcactt tttatcatgt actttttgtt gcgtgataaa cttctatttt
     4741 cgccaaaaat gaaaaaacaa acttctttct ttcttgaaaa ttaagaaatg tcatttttgg
     4801 gttttttttt tgttttgtta aagtgaaaca aaattctagc ggggatcaat tattggataa
     4861 attaatcata tagttatcag acacaacata ttcatgattt catattacac tagatatctt
     4921 cttcaatttt aatttatttg cgcgatttta aaacaccaaa ttaatctaca ccttatgcac
     4981 ttttatatat ataactgtta attcattttc tatgattaaa tttcgagaga tgtattcaat
     5041 caaacatttt aagtgatttg tgttaaaata acaaatccaa tgttattcaa ttatggattt
     5101 ctaaaaatct catgaaatct aatgttattg aatttatgat tttaaaattt actttaaaat
     5161 cccatgttat taaaaacatt ttaaggatta gattattact gattttaaga aacttaagta
     5221 aattttagtg gatttcctta gttaaaaata cagaatttta aatctcatgg tcttaggtgg
     5281 aaatcgaaaa gatttaacaa taaatcataa caacttcttt aaatttatcc aaattcttca
     5341 aaattacata aaaactcaaa ataacttaaa tcactcaaaa tagaagatta aatacactca
     5401 tatttttaga aaaattctaa tcactaaatg aagcgaaaaa gacatgcatt acgtattgaa
     5461 acgaaattga tctgtcatta tttggttcac aagatttgtt ataccttcac tttcaagatt
     5521 aagacataga tatcatccac aacacaacat ctttgactcc ttgactctgc tgataatgaa
     5581 acagtcacgg gtctcaacac gacatcgttt ttgattcttt ttatgttcta attttgtcat
     5641 tgtccacttc tttgacactc ttaaaaaaat tacagattta tcatttaatt agttaaacat
     5701 tactaaatca aatacatcga ctgatagaat ggcaatgatc catttataat ctaaaactaa
     5761 attagacaca gatccgttgt ctgtgcttcc atttaatgtt cttctcatag tttcgatata
     5821 caaaataaaa taatatcgaa taaataagtt ttgtgttaaa aaaaaaaatc gaataaataa
     5881 gtgaataact aataatgata tatcaaataa aaagtcaaaa tgtgaaaagt attaaaacta
     5941 atcatgcaca taattgattc tgtttttctg tgtcttacta tctattggtc tttgacaact
     6001 ctaacacaat cacgtgaaca aatctcattt cattaactaa tcaatgggat ccaaagacag
     6061 ctaattattc attaccaaat gcatcatcat ttcaatcatt tgaataagcg aacgcgtttt
     6121 tatgttaaat aattttgtat tgaagatgtg gccgtctctg ataaaaaaga caaaggaagg
     6181 aggcatcgat gtgatccaaa cgtacgtttt ctggaacctc catgagccta agcttggaca
     6241 ggtccgttcc atttatgatt tctcaatatg acttttaaaa tttttcattt tcttcatatg
     6301 gtaggaaaat tatagaatat ataattactt cgaataaaat gaatattgca gtatgatttt
     6361 agtggaagaa acgatttagt gaagtttatt aaagaaatta gatcacaagg tctctatgtc
     6421 tgtctacgta ttggaccctt catcgaagcc gagtggaatt acgggtacta atcaactgac
     6481 caaaattatt gtttgtcaac gtatatttaa cattgaaatg attttgacga ccggtttgat
     6541 taaccagggg attgccattt tggttacggg atgtacccgg tatggtatac cgaaccgaca
     6601 atgagccctt caagttccac atgcaaaaat tcaccgctaa gatcgtagat ttgatgaaat
     6661 ctgagggctt atatgcttct caaggaggtc caattattct ttctcaggta tttaatcttt
     6721 tcaatcttat tcgtcaaatt caatgtcacc aaaccgaacc aaatcctttg cattaaatac
     6781 attaaccgga tcattgctta gacaaataag tttcttattt gggttctttt gtaagatcga
     6841 gaacgagtat gcaaacgtgg agggggcttt ccatgagaaa ggagcatcgt acatcaagtg
     6901 ggcaggtcaa atggcggtcg ggcttaagac cggagtgcca tggatcatgt gcaagtcgcc
     6961 cgacgctcct gatcccgtgg ttcgtaccgg ttacttatct aagatcaatc atcaatgact
     7021 caatgtttct tatacttctt agagatcgaa tattttcatc tgtagatcaa tacctgcaac
     7081 gggatgaaat gcggcgagac ttttcctgga ccaaactcac ccaataagcc gaaaatgtgg
     7141 actgaggatt ggacttcatt gtaagggttt tacttcattg tctatttcga taggtttctt
     7201 gttttaaaag ttaactatgt ttattttatt tttttgtgat gaaaaagctt tcaggtttat
     7261 ggaaaagagc cttatattag atctgctgag gatattgctt tccatgccgc tcttttcgtt
     7321 gcaaaaaatg gaagttacat caactattac atggtaaata acaacatctt tatcaaatat
     7381 tagtcgcatt atatgatcat caataactca ccaccttgtt tcaatacagt accatggtgg
     7441 aacaaacttt ggaaggactt catcttcgta tttcattaca ggatactatg atcaagctcc
     7501 tctcgacgaa tacggtttgg tctaaatgca tgttctctgt ttgaaagaac ctgataattt
     7561 gattctgatt ttgttttgtg tgtttcaggt cttttaagac agcctaagta cggtcatttg
     7621 aaggagcttc acgctgcgat aaaatcctct gcaaatcctc tgcttcaagg aaagcaaacg
     7681 attctctcct tgggaccaat gcaacaagct tatgtctttg aagacgcgaa caatggatgc
     7741 gttgcgtttc tggtaaacaa tgacgctaaa gccagtcaaa tacaattcag aaacaatgcg
     7801 tatagcttga gtcccaagtc aattggtatt cttcagaact gcaaaaacct aatttatgaa
     7861 actgcaaaag taagtctttt tgcgacctta cagatcatac taatagtaat caacaactat
     7921 tcaagcattt taaccgctta attaataaag gtgaatgtga agatgaatac gagagttaca
     7981 acgccggttc aagtgttcaa cgttcccgat aactggaatt tgtttagaga aacaatccct
     8041 gcattcccag gcacatcttt gaagacaaat gctttgttgg aacacactaa tttgacaaag
     8101 gacaaaactg attatctttg gtacacttca aggtaaaata ttatagacat tacacattac
     8161 atttctaatg ttcatttatc ttttctaacg caagtttggc tcttccaatg taagctttaa
     8221 gctggattca ccctgcacga acccgtctat ctacacagaa tcatcaggac atgttgtcca
     8281 cgtgttcgtc aacaatgcac tagcaggtac ataagtattg agagtaacaa cggggaagaa
     8341 aaatgtgttc acaaactctg cttcggtgtt gattttgacg gtccattgca cttagtttgc
     8401 aagatacaca tactttactc tctagtatag cttaactacc aaataattca atattcatat
     8461 ttatgaacat attaaaagcg tgttgttgta ggatctggac atggaagcag agacataagg
     8521 gtagttaaac tacaagctcc ggttagctta atcaatggac aaaacaatat atcgatcctc
     8581 agcggcatgg ttggtctacc ggtaagacgt ttactgatac tagaaccctg tttattaagt
     8641 gagtagatag aatcataaca tttttttcaa ccttaataat aaaaggactc tggagcttac
     8701 atggagagga gatcttatgg actaaccaaa gtacaaatta gctgtggcgg gacaaaaccc
     8761 atcgatttga gtagatctca atggggttac tcggtaatat gatgaataag cctttcattt
     8821 tatgagtgat ggaagattgt gttgatacat aacctagtat gtatatgtgt tgtgaaggtg
     8881 ggtttgcttg gagagaaagt taggttatat caatggaaga atttaaatag agtgaagtgg
     8941 agtatgaata aagctggact aattaagaac cgccccctcg cttggtacaa ggtatgccta
     9001 tttacatatg taaataagta ctatatgtgt aatttatgaa tagtatcggt gacgaatgtg
     9061 aatatgcaga caacgtttga tgggccgaat ggagatgggc ccgtgggcct acacatgtcg
     9121 agtatgggta aaggagaaat ttgggtgaat ggtgaaagca ttggtcgtta ttgggtctcg
     9181 tttctcaccc ctgctggaca accttctcaa tctatgtaag tgtcacacac acacctggaa
     9241 acacaaaatt atgtttgttg ttactttttg atatggaatt ggatttggtt aatgcagata
     9301 tcatattcct cgggcgttct taaaaccatc tggaaattta ttggtggtat ttgaagagga
     9361 aggtggtgat cctcttggga tatctttgaa tacaatatcg gttgttggtt cgagtcaagc
     9421 tcaatctcaa ttctcctgcc tgcagcctgg aggtggaggt ggagctgtga gcaagggcga
     9481 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     9541 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     9601 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     9661 ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     9721 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     9781 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     9841 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     9901 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     9961 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
    10021 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
    10081 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
    10141 cgccgccggg atcactctcg gcatggacga gctgtacaag gatcctgctg gtgctgctgc
    10201 gcctctagca taatctagag aatttccccg atcgttcaaa catttggcaa taaagtttct
    10261 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
    10321 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
    10381 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
    10441 aggataaatt atcgcgcgcg gtgtcatcta tgttactgag ctccagcttt tgttcccttt
    10501 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    10561 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10621 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10681 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10741 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10801 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10861 ataacgcagg aaagaacatg aaggccttga caggatatat tggcgggtaa actaagtcgc
    10921 tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    10981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    11041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    11101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    11161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    11221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    11281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    11341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    11401 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    11461 gcgctctgct gaagccagtt accttcggaa gaagagttgg tagctcttga tccggcaaac
    11521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    11581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    11641 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    11701 taaattaaaa atgaagtttt aaatcaatct aaagtactga tgaatcccct aatgattttg
    11761 gtaaaaatca ttaagttaag gtggatacac atcttgtcat atgatcaaat ggtttcgcga
    11821 aaaatcaata atcagacaac aagatgtgcg aactcgatat tttacacgac tctctttacc
    11881 aattctgccc cgaattacac ttaaaacgac tcaacagctt aacgttggct tgccacgcat
    11941 tacttgactg taaaactctc actcttaccg aacttggccg taacctgcca accaaagcga
    12001 gaacaaaaca taacatcaaa cgaatcgacc gattgttagg taatcgtcac ctccacaaag
    12061 agcgactcgc tgtataccgt tggcatgcta gctttatctg ttcgggcaat acgatgccca
    12121 ttgtacttgt tgactggtct gatattcgtg agcaaaaacg acttatggta ttgcgagctt
    12181 cagtcgcact acacggtcgt tctgttactc tttatgagaa agcgttcccg ctttcagagc
    12241 aatgttcaaa gaaagctcat gaccaatttc tagccgacct tgcgagcatt ctaccgagta
    12301 acaccacacc gctcattgtc agtgatgctg gctttaaagt gccatggtat aaatccgttg
    12361 agaagctggg ttggtactgg ttaagtcgag taagaggaaa agtacaatat gcagacctag
    12421 gagcggaaaa ctggaaacct atcagcaact tacatgatat gtcatctagt cactcaaaga
    12481 ctttaggcta taagaggctg actaaaagca atccaatctc atgccaaatt ctattgtata
    12541 aatctcgctc taaaggccga aaaaatcagc gctcgacacg gactcattgt caccacccgt
    12601 cacctaaaat ctactcagcg tcggcaaagg agccatgggt tctagcaact aacttacctg
    12661 ttgaaattcg aacacccaaa caacttgtta atatctattc gaagcgaatg cagattgaag
    12721 aaaccttccg agacttgaaa agtcctgcct acggactagg cctacgccat agccgaacga
    12781 gcagctcaga gcgttttgat atcatgctgc taatcgccct gatgcttcaa ctaacatgtt
    12841 ggcttgcggg cgttcatgct cagaaacaag gttgggacaa gcacttccag gctaacacag
    12901 tcagaaatcg aaacgtactc tcaacagttc gcttaggcat ggaagttttg cggcattctg
    12961 gctacacaat aacaagggaa gacttactcg tggctgcaac cctactagct caaaatttat
    13021 tcacacatgg ttacgctttg gggaaattat gaggggatct ctcagtctaa agtatatatg
    13081 tgtaacattg gtctagtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
    13141 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
    13201 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
    13261 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
    13321 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
    13381 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
    13441 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
    13501 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
    13561 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
    13621 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
    13681 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
    13741 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg
    13801 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
    13861 tgttggaatt taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacac
    13921 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
    13981 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
    14041 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
    14101 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caa
//
